&amp;lt;i&amp;gt;Toxoplasma gondii&amp;lt;/i&amp;gt; B1 Gene Detection in Feces of Stray Cats around Seoul, Korea and Genotype Analysis of Two Laboratory-Passaged Isolates

Jung, Byung Mun; Lee, Sang Eun; Lim, Hyemi; Cho, Jaeeun; Kim, Deok Gyu; Song, Hyemi; Kim, Min Jae; Shin, Eun Hee; Chai, Jong Yil

doi:10.3347/kjp.2015.53.3.259

Cited by 21 publications

(30 citation statements)

References 16 publications

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“…This is low in comparison with other studies, like the one performed in Portugal, where 20.5% prevalence was found in domestic cats feces samples using the B1 sequence in PCR [14]. However, our study found a higher prevalence compared to that observed in a Korean study, where prevalence was 4.5% [20], and a study in Switzerland, where prevalence was 0.4% [4]. This is the first study in Colombia detecting T. gondii DNA from cats feces samples, but the frequency of positive samples may be lower compared to previous studies with oocyst prevalence reported at 66.6% [30] and seroprevalence at 84.8%-89.3% [10,30].…”

Section: Discussioncontrasting

confidence: 99%

“…Wild and domestic felids are the only known definitive hosts with the ability to shed oocysts in their feces. Common pathways of infection include oocyst-contaminated water, soil, and food; tissue cysts in undercooked or raw meat; and congenital transmission [13,20,37,43]. Oocysts are the environmentally resistant form *Corresponding author: oazamorav@uqvirtual.edu.co Parasite 27, 25 (2020) Ó A. Zamora-Vélez et al, published by EDP Sciences, 2020 https://doi.org/10.1051/parasite/2020023 Available online at: www.parasite-journal.org of the parasite and play a key role in transmission to new hosts and ecosystems, generating the need to study humans alongside domestic and wild animal populations [43].…”

Section: Introductionmentioning

confidence: 99%

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Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia

et al. 2020

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The high prevalence of Toxoplasma gondii in the human population in Colombia has been linked to the existence of a high density of urban stray cats, exposing the whole population to a high density of oocysts. The goal of this study was to determine the DNA prevalence of T. gondii by conventional PCR and to phylogenetically analyze ROP18 sequences from positive samples in domestic cat (Felis catus) fecal samples in the city of Armenia, Quindío. Fecal samples from 140 cats were collected from 10 districts around the city. Samples were concentrated using Ritchie’s method and analyzed through optical microscopy. Concentrates were used for DNA extraction followed by nested PCR amplification for T. gondii gene B1. PCR for ROP18 was performed on all B1 positive samples; the ROP18 sequences obtained were related to the Archetype I Brazilian and Chinese strains. No oocysts were detected by optical microscopy; however, 17.8% (25/140) B1 and 24% (6/25) ROP18 PCR-positive samples were detected. Phylogenetic analyses showed that isolates clustered into a single group. We assessed whether associations existed between T. gondii positive fecal samples and survey variables such as cat healthcare and socioeconomic characteristics of owners, but no statistically significant associations were found. The presence of T. gondii in cat feces is an important factor contributing to the high prevalence in the human population of this city.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia

et al. 2020

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“…This result may be due to consumption of meat contaminated with Toxoplasma cysts or due to the small number of Toxoplasma oocysts in fecal specimens which are difficult to determine by fecal examination. Similar to our results, a low prevalence of oocysts shedding in cats was determined at 1.2% in Iran [16], at 2.3% in Italy [17], at 0.3% in Japan [18], at 0.14 % in Germany [19], at 0.4% in Switzerland [20], at 4.7% in South Korea [21], at 0.9 in the USA [22], at 0.8% in Thailand [23], at 0.76 % in Finland [24] by microscopy and PCR methods. In contrast to serological studies, examining feces did not show any information about age and gender as risk factors, due to the low prevalence of oocyst shedding by the cats [13,25].…”

Section: Discussionsupporting

confidence: 91%

Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran.

Khodaverdi

Razmi

2019

Preprint

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Background: Cats as a definitive host have an important role in the epidemiology of toxoplasmosis in humans and animals. The aim of the study was to determine the frequency of Toxoplasma gondii infection and isolate and identify the genotypes of T. gondii in stray cats in the Mashhad suburb. Methods: From April 2016 to August 2017, 175 fecal samples from stray cats and 31 brain samples from cats killed in driving accidents were collected. The fecal samples were examined by fecal flotation technique and T. gondii-specific PCR. The brain samples were investigated by T. gondii-specific PCR and consequently examined by mice bioassay. The DNA of T. gondii isolated was genotyped using SAG1, SAG2, SAG3, BTUB and GRA6 as PCR-restriction fragment length polymorphism (PCR-RFLP) markers. Results: In the present study, Toxoplasma-like oocysts were microscopically observed in 2.2% (4/175) fecal samples. The presence of Toxoplasma oocysts was confirmed in one microscopy-positive sample by PCR. In addition, T. gondii DNA was detected in 4% (7/175) microscopy-negative samples using PCR. T. gondii was isolated from one brain PCR-positive sample by mice bioassay. The isolate was avirulent and many T. gondii cysts were observed in mice brain. The isolate was successfully genotyped by PCR-RLFP analysis .The isolated genotyped was type II. Besides, eight Toxoplasma-positive fecal samples contained insufficient DNA and only amplified at SAG-3 locus in PCR. These samples were also showed type II pattern at this locus. Conclusions: Parasitological and molecular results showed low frequency of Toxoplasma infection in the stray cats, and identified the genotype of T. gondii isolate as type II, for the first time in Mashhad area, Khorasan Razavi Province.

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“…Even in the same country, the rate of positivity varies considerably depending on where the sample was taken, which test method was used, etc [10,[19][20][21]. In Korea, many researchers have investigated the status of feline toxoplasmosis infection [10,[19][20][21][22][23][24]. However, 1 study regarding the status of infection with toxoplasmosis in dogs has been conducted during 2006-2007 [22].…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea

et al. 2020

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The outbreak of human toxoplasmosis can be attributed to ingestion of food contaminated with Toxoplasma gondii. Toxoplasmosis recently increased in domestic and stray dogs and cats. It prompted studies on the zoonotic infectious diseases transmitted via these animals. Sero- and antigen prevalences of T. gondii in dogs and cats were surveyed using ELISA and PCR, and B1 gene phylogeny was analyzed in this study. Toxoplasmosis antibodies were measured on sera of 403 stray cats, 947 stray dogs, 909 domestic cats, and 2,412 domestic dogs collected at nationwide regions, Korea from 2017 to 2019. In addition, whole blood, feces, and tissue samples were also collected from stray cats (1,392), stray dogs (686), domestic cats (3,040), and domestic dogs (1,974), and T. gondii-specific B1 gene PCR was performed. Antibody prevalence of stray cats, stray dogs, domestic cats, and domestic dogs were 14.1%, 5.6%, 2.3%, and 0.04%, respectively. Antigen prevalence of these animals was 0.5%, 0.2%, 0.1%, and 0.4%, respectively. Stray cats revealed the highest infection rate of toxoplasmosis, followed by stray dogs, domestic cats, and domestic dogs. B1 gene positives were 5 of stray cats, and identified to high/moderate pathogenic Type I/III group. These findings enforce that preventive hygienic measure should be strengthened at One Health level in dogs and cats, domestic and stray, to minimize human toxoplasmosis infections.

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<i>Toxoplasma gondii</i> B1 Gene Detection in Feces of Stray Cats around Seoul, Korea and Genotype Analysis of Two Laboratory-Passaged Isolates

Cited by 21 publications

References 16 publications

Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia

Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia

Prevalence and genotyping of Toxoplasma gondii in stray cats in Mashhad area, Iran.

Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea

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