&amp;lt;italic&amp;gt;GhHyPRP4&amp;lt;/italic&amp;gt;, a cotton gene encoding putative hybrid proline-rich protein, is preferentially expressed in leaves and involved in plant response to cold stress

Huang, Geng-Qing; Gong, Si-Ying; Xu, Wen‐Liang; Zhang, Dejing; Qin, Lixia; Li, Wen; Li, Xuebao

doi:10.1093/abbs/gmr040

Cited by 31 publications

(26 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, MfHyPRP transcript was induced by cold, dehydration and ABA, but not responsive to salt stress. This response is different from that of MsACIC and other reported HyPRPs : MsACIC transcript is induced by cold, but not responsive to drought, heat and ABA treatment (Castonguay et al ); SbPRP is induced by drought and salt, but inhibited by ABA (He et al ); CcHyPRP transcript is induced by cold, drought and salt stresses as well as ABA treatment (Priyanka et al ); GhHyPRP4 expression is induced by cold stress, but reduced by drought, salt and ABA (Huang et al ). Although MfHyPRP and MsACIC share high identity in sequence, they showed different response to drought and ABA treatment.…”

Section: Discussionmentioning

confidence: 82%

“…falcata in this study. MfHyPRP was most homologous to MsACIC (Castonguay et al ), and showed a high similarity with SbPRP (He et al ) and GhHyPRP4 (Huang et al ). It exhibited a typical structure of HyPRP consisting of a signal peptide, PRD at N‐terminus and a conserved 8CM at C‐terminus.…”

Section: Discussionmentioning

confidence: 99%

“…The role of HyPRP in tolerance to PEG and mannitol induced osmotic stress has been observed in Arabidopsis and yeast by overexpressing CcHyPRP , but cold tolerance is not altered (Priyanka et al ). On the contrary, GhHyPRP4 and EARLI1 are demonstrated to be related to cold tolerance (Zhang and Schläppi , Huang et al ). Abiotic stress, such as cold and drought, results in production of reactive oxygen species (ROS) and induces oxidative damages of plants, whereas antioxidant system functions to protect plant cell by scavenging the accumulated ROS under stressed conditions (Foyer and Noctor ).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Nitric oxide mediates cold‐ and dehydration‐induced expression of a novel MfHyPRP that confers tolerance to abiotic stress

Tan

Zhuo

Guo

2013

Physiologia Plantarum

View full text Add to dashboard Cite

Hybrid proline-rich proteins (HyPRPs) are cell wall-localized proteins, and are frequently responsive to environmental stresses. The coding sequence of a HyPRP cDNA was isolated from Medicago falcata, a forage crop that shows cold and drought tolerance. The predicted MfHyPRP contains a proline-rich domain at N-terminus after the signal peptide and a conserved eight-cysteine motif at the C-terminus. Higher level of MfHyPRP transcript was observed in leaves than in stems and roots under control conditions, while more MfHyPRP transcript was induced in leaves and stems than in roots after cold treatment. Levels of MfHyPRP transcript and MfHyPRP protein in leaves were induced by cold, dehydration, abscisic acid (ABA), hydrogen peroxide (H2 O2) and nitric oxide (NO), but not responsive to salt stress. The cold- or dehydration-induced expression of MfHyPRP was blocked by scavenger of NO, but not affected by inhibitor of ABA biosynthesis or scavenger of H2 O2. The results indicated that NO, but not ABA and H2 O2, was essential in the cold- and dehydration-induced expression of MfHyPRP. Overexpression of MfHyPRP in tobacco led to increased tolerance to freezing, chilling and osmotic stress as well as methyl viologen-induced oxidative stress. The increased cold and osmotic stress tolerance was proposed to be associated with improved protection against oxidative damages. It is suggested that NO mediates cold- and dehydration-induced expression of MfHyPRP that confers tolerance to abiotic stress.

show abstract

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Nitric oxide mediates cold‐ and dehydration‐induced expression of a novel MfHyPRP that confers tolerance to abiotic stress

Tan

Zhuo

Guo

2013

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…In previous studies, these genes also showed stable expression in several species. For instance, H3 , ACTB and UBQ have been used as internal controls for the analysis of RT-qPCR in cotton (Wang, Wang & Zhang, 2013; Huang et al, 2011). In maize, TUB was one of best reference genes for gene expression (Lin et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Cloning and evaluation of reference genes for quantitative real-time PCR analysis inAmorphophallus

Wang

Niu

Wang

et al. 2017

PeerJ

View full text Add to dashboard Cite

Quantitative real-time reverse transcription PCR (RT-qPCR) has been widely used in the detection and quantification of gene expression levels because of its high accuracy, sensitivity, and reproducibility as well as its large dynamic range. However, the reliability and accuracy of RT-qPCR depends on accurate transcript normalization using stably expressed reference genes. Amorphophallus is a perennial plant with a high content of konjac glucomannan (KGM) in its corm. This crop has been used as a food source and as a traditional medicine for thousands of years. Without adequate knowledge of gene expression profiles, there has been no report of validated reference genes in Amorphophallus. In this study, nine genes that are usually used as reference genes in other crops were selected as candidate reference genes. These putative sequences of these genes Amorphophallus were cloned by the use of degenerate primers. The expression stability of each gene was assessed in different tissues and under two abiotic stresses (heat and waterlogging) in A. albus and A. konjac. Three distinct algorithms were used to evaluate the expression stability of the candidate reference genes. The results demonstrated that EF1-a, EIF4A, H3 and UBQ were the best reference genes under heat stress in Amorphophallus. Furthermore, EF1-a, EIF4A, TUB, and RP were the best reference genes in waterlogged conditions. By comparing different tissues from all samples, we determined that EF1-α, EIF4A, and CYP were stable in these sets. In addition, the suitability of these reference genes was confirmed by validating the expression of a gene encoding the small heat shock protein SHSP, which is related to heat stress in Amorphophallus. In sum, EF1-α and EIF4A were the two best reference genes for normalizing mRNA levels in different tissues and under various stress treatments, and we suggest using one of these genes in combination with 1 or 2 reference genes associated with different biological processes to normalize gene expression. Our results will provide researchers with appropriate reference genes for further gene expression quantification using RT-qPCR in Amorphophallus.

show abstract

“…Although huge progress has been made in understanding the molecular mechanisms underlying HyPRP action in several plants (Deutch and Winicov, 1995; Richards and Gardner, 1995; Goodwin et al , 1996; Josè-Estanyol and Puigdomènech, 1998; Wilkosz and Schläppi, 2000; Bubier and Schläppi, 2004; Zhang and Schläppi, 2007; Priyanka et al , 2010; Dvoráková et al, 2011; Huang et al , 2011; Xu et al , 2011), the roles of the soybean HyPRP gene family still remain largely unknown. The sequencing and assembly of the soybean genome (Schmutz et al , 2010) may provide new approaches for identifying protein-coding loci possibly involved in the ability of soybean to survive stressful conditions.…”

Section: Introductionmentioning

confidence: 99%

Identification of the soybean HyPRP family and specific gene response to Asian soybean rust disease

et al. 2013

View full text Add to dashboard Cite

Soybean [Glycine max (L.) Merril], one of the most important crop species in the world, is very susceptible to abiotic and biotic stress. Soybean plants have developed a variety of molecular mechanisms that help them survive stressful conditions. Hybrid proline-rich proteins (HyPRPs) constitute a family of cell-wall proteins with a variable N-terminal domain and conserved C-terminal domain that is phylogenetically related to non-specific lipid transfer proteins. Members of the HyPRP family are involved in basic cellular processes and their expression and activity are modulated by environmental factors. In this study, microarray analysis and real time RT-qPCR were used to identify putative HyPRP genes in the soybean genome and to assess their expression in different plant tissues. Some of the genes were also analyzed by time-course real time RT-qPCR in response to infection by Phakopsora pachyrhizi, the causal agent of Asian soybean rust disease. Our findings indicate that the time of induction of a defense pathway is crucial in triggering the soybean resistance response to P. pachyrhizi. This is the first study to identify the soybean HyPRP group B family and to analyze disease-responsive GmHyPRP during infection by P. pachyrhizi.

show abstract

<italic>GhHyPRP4</italic>, a cotton gene encoding putative hybrid proline-rich protein, is preferentially expressed in leaves and involved in plant response to cold stress

Cited by 31 publications

References 28 publications

Nitric oxide mediates cold‐ and dehydration‐induced expression of a novel MfHyPRP that confers tolerance to abiotic stress

Nitric oxide mediates cold‐ and dehydration‐induced expression of a novel MfHyPRP that confers tolerance to abiotic stress

Cloning and evaluation of reference genes for quantitative real-time PCR analysis inAmorphophallus

Identification of the soybean HyPRP family and specific gene response to Asian soybean rust disease

Contact Info

Product

Resources

About