Ampelomyces mycoparasites from apple powdery mildew identified as a distinct group based on single-stranded conformation polymorphism analysis of the rDNA ITS region

Szentiványi, Orsolya; Kiss, Levente; Russell, John C.; Kovács, Gábor M.; Varga, Krisztina; Jankovics, Tünde; Lesemann, Silke; Xu, Xiangming; Jeffries, Peter

doi:10.1017/s0953756204001820

Cited by 45 publications

(53 citation statements)

References 22 publications

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“…DNAs of KTP-05 and KMP-01 were extracted from conidia using a Qiagen DNeasy plant mini kit (Qiagen, Hilden, Germany), and ribosomal DNA internal transcribed spacer (ITS) sequences were amplified by polymerase chain reaction (PCR), using the ITS1F (Gardes & Bruns 1993) and ITS4 fungalspecific primers (White et al 1990). PCR was carried out with an iCycler (Bio-Rad, Hercules, USA) according to the protocols of Szentiványi et al (2005). The nucleotide sequences of both isolates were deposited at the DNA Data Bank of Japan (DDBJ) under the accession numbers AB808656 for KTP-05 and AB808657 for KMP-01.…”

Section: Methodsmentioning

confidence: 99%

Natural woody plant, Mallotus japonicus, as an ecological partner to transfer different pathotypic conidia of Oidium neolycopersici to greenhouse tomatoes

Nonomura¹,

Matsuda²,

Yamashita³

et al. 2013

Plant Prot. Sci.

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Nonomura T., Matsuda Y., Yamashita S., Akahoshi H., Takikawa Y., Kakutani K., Toyoda H. (2013): Natural woody plant, Mallotus japonicus, as an ecological partner to transfer different pathotypic conidia of Oidium neolycopersici to greenhouse tomatoes. Plant Protect. Sci., 49 (Special Issue): S33-S40.In our routine surveys for the powdery mildew disease in greenhouse tomatoes, we detected a new pathogen that forms pseudochains consisting of 12 conidia. To identify the original plant that dispersed this pathogen, wild plants infected with powdery mildew were monitored. The pathogen on Japanese mallotus, Mallotus japonicus, produced a similar type of pseudochain, and conidia were infectious to tomatoes. Inversely, the conidia on the tomato leaves infected M. japonicus. Infectivity assays and internal transcribed spacer (ITS)-based phylogenetic analyses indicated that the two pathogens on the tomato and M. japonicus were identical. These results suggest that the conidia on M. japonicus can be transmitted to greenhouse tomatoes. This work documents the ecological transmission of conidia between wild plants and greenhouse tomatoes.

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Section: Methodsmentioning

confidence: 99%

Natural woody plant, Mallotus japonicus, as an ecological partner to transfer different pathotypic conidia of Oidium neolycopersici to greenhouse tomatoes

Nonomura¹,

Matsuda²,

Yamashita³

et al. 2013

Plant Prot. Sci.

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“…The rDNA ITS region was amplified using the ITS1F/ITS4 fungal-specific primer pair as described in Szentiványi et al (60). PCR products were purified using a PCR-M Clean Up System (Viogene) and sequenced using a BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA), according to the instructions of the manufacturer.…”

Section: Methodsmentioning

confidence: 99%

Oidium neolycopersici: Intraspecific Variability Inferred from Amplified Fragment Length Polymorphism Analysis and Relationship with Closely Related Powdery Mildew Fungi Infecting Various Plant Species

Jankovics¹,

Bai²,

Kovács³

et al. 2008

Phytopathology®

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Jankovics, T., Bai, Y., Kovács, G. M., Bardin, M., Nicot, P. C., Toyoda, H., Matsuda, Y., Niks, R. E., and Kiss, L. 2008. Oidium neolycopersici: Intraspecific variability inferred from amplified fragment length polymorphism analysis and relationship with closely related powdery mildew fungi infecting various plant species. Phytopathology 98:529-540.Previous works indicated a considerable variation in the pathogenicity, virulence, and host range of Oidium neolycopersici isolates causing tomato powdery mildew epidemics in many parts of the world. In this study, rDNA internal transcribed spacer (ITS) sequences, and amplified fragment length polymorphism (AFLP) patterns were analyzed in 17 O. neolycopersici samples collected in Europe, North America, and Japan, including those which overcame some of the tomato major resistance genes. The ITS sequences were identical in all 10 samples tested and were also identical to ITS sequences of eight previously studied O. neolycopersici specimens. The AFLP analysis revealed a high genetic diversity in O. neolycopersici and indicated that all 17 samples represented different genotypes. This might suggest the existence of either a yet unrevealed sexual reproduction or other genetic mechanisms that maintain a high genetic variability in O. neolycopersici. No clear correlation was found between the virulence and the AFLP patterns of the O. neolycopersici isolates studied. The relationship between O. neolycopersici and powdery mildew anamorphs infecting Aquilegia vulgaris, Chelidonium majus, Passiflora caerulea, and Sedum alboroseum was also investigated. These anamorphs are morphologically indistinguishable from and phylogenetically closely related to O. neolycopersici. The crossinoculation tests and the analyses of ITS sequences and AFLP patterns jointly indicated that the powdery mildew anamorphs collected from the above mentioned plant species all represent distinct, but closely related species according to the phylogenetic species recognition. All these species were pathogenic only to their original host plant species, except O. neolycopersici which infected S. alboroseum, tobacco, petunia, and Arabidopsis thaliana, in addition to tomato, in cross-inoculation tests. This is the first genome-wide study that investigates the relationships among powdery mildews that are closely related based on ITS sequences and morphology. The results indicate that morphologically indistinguishable powdery mildews that differed in only one to five single nucleotide positions in their ITS region are to be considered as different taxa with distinct host ranges.

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“…Polymerase chain reaction (PCR) was carried out in an iCycler (Bio-Rad, Hercules, CA, USA) using the ITS1F and ITS4 fungal-specific primers designed by Gardes and Bruns (1993) and White et al (1990), respectively. PCR was performed according to the protocols described by Szentiványi et al (2005): 5 min denaturation at 95°C and 35 cycles, consisting of a 30 s denaturation at 95°C, annealing at 52°C, and extension at 72°C, were followed by a final extension cycle of 5 min at 71°C. The nucleotide sequence of the amplified region was determined using the BigDye Terminator Cycle Sequencing Ready Reaction Kit from Applied Biosystems (Tokyo, Japan) on an ABI Prism 3130 Genetic Analyzer (Applied Biosystems) in the Pharmaceutical Research and Technology Institute, Kinki University, Japan.…”

Section: Sequencing Of the Rdna Its Regionmentioning

confidence: 99%

Conidia of Erysiphe trifoliorum attempt penetration twice during a two-step germination process on non-host barley leaves and an artificial hydrophobic surface

et al. 2011

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In the present study, using a high-fidelity digital microscope, we observed the sequence of appressorial development on the germ tubes of a powdery mildew fungus isolated from red clover leaves. Based on its morphological characteristics and rDNA internal transcribed spacer (ITS) sequences, the fungus was identified as Erysiphe trifoliorum, and one of its isolates, designated as KRCP-4N, was used in this work. The conidial germination of isolate KRCP-4N was studied on host (red clover) and non-host (barley) leaves, as well as on an artificial hydrophobic membrane (Parafilm). More than 90% of conidia germinated synchronously and developed dichotomous appressoria (symmetrical double-headed appressoria) on all substrata used. On host leaves, all appressorium-forming conidia developed hyphae (colonyforming hyphae) from conidial bodies without extending germ tubes from the tips of the appressoria. On non-host leaves and on Parafilm-covered glass slides, however, all conidia extended germ tubes from one side of dichotomous appressoria (two-step germination). In addition to the dichotomous appressoria, we detected a few conidia that produced hooked appressoria and extended germ tubes from the tip of the appressorium. Penetration attempts by KRCP-4N conidia on barley leaves were impeded by papillae formed at penetration sites beneath these two types of appressorium. From these results, we conclude that the ''two-step germination'' of E. trifoliorum KRCP-4N conidia is the result of an unsuccessful penetration attempt, causing diversity in appressorial shape.

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Ampelomyces mycoparasites from apple powdery mildew identified as a distinct group based on single-stranded conformation polymorphism analysis of the rDNA ITS region

Cited by 45 publications

References 22 publications

Natural woody plant, Mallotus japonicus, as an ecological partner to transfer different pathotypic conidia of Oidium neolycopersici to greenhouse tomatoes

Natural woody plant, Mallotus japonicus, as an ecological partner to transfer different pathotypic conidia of Oidium neolycopersici to greenhouse tomatoes

Oidium neolycopersici: Intraspecific Variability Inferred from Amplified Fragment Length Polymorphism Analysis and Relationship with Closely Related Powdery Mildew Fungi Infecting Various Plant Species

Conidia of Erysiphe trifoliorum attempt penetration twice during a two-step germination process on non-host barley leaves and an artificial hydrophobic surface

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