Amperometric biosensor based on tyrosinase immobilized on a boron-doped diamond electrode

Zhou, Yan; Tian, Ru Hai; Zhi, Jin

doi:10.1016/j.bios.2006.03.001

Cited by 113 publications

(41 citation statements)

References 36 publications

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“…The electrochemical behavior of DA may be very close to two consecutive singleelectron processes. The steps of the enzymatic reaction were shown as follows [20 [21], when the Tyr was immobilized on the GCE with CHIT, extra electron mediators such as Fe(CN) 6 4− had to be employed.…”

Section: Electrochemical Sensing Characteristics Of Tyr-fe 3 O 4 -Chimentioning

confidence: 99%

“…Electrochemical techniques are an attractive method for the determination of DA because of their high sensitivity as well as their applicability to real-time detection of DA in brain tissues. Tyrosinase (Tyr) based electrochemical biosensor is a promising and effective tool for the determination of DA [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…The key issue in the development of Tyr biosensor is the effective immobilization of Tyr on the electrode surface. Different approaches, such as polymer entrapment [7], electropolymerization [8], sol-gels [9], self-assembled monolayer [10], and covalent linking [6] have been widely used to construct Tyr biosensors. However, some of these methods are relatively complex.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Dopamine Based on Tyrosinase-Fe3O4 Nanoparticles-chitosan Nanocomposite Biosensor

Zhang¹,

Yang²,

Xu³

et al. 2010

Am. J. Biomed. Sci.

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A tyrosinase biosensor based on Fe 3 O 4 -chitosan nanocomposite has been developed for the amperometric detection of dopamine by the biocatalytically liberated dopaquinone at −0.25V vs. saturated calomel electrode. The obtained bio-nanoparticles, which were attached to the surface of a glassy carbon electrode (GCE), showed excellent electrochemical characteristics and at the same time acted as mediator to transfer electrons between the enzyme and the electrode. Under optimal conditions, the biosensor showed broad linear response of 2.0×10 −8 to 7.5×10 −5 mol L −1 , with the low detection limit of 6.0×10 −9 mol L −1 and the high sensitivity of 46 μA/mM for the determination of dopamine in the presence of ascorbic acid. Such tyrosinase biosensor exhibits great promise for rapid, simple and cost-effective analysis of dopamine in the samples. This immobilization approach effectively improved the stability of the electron transfer mediator and is promising for construction of biosensor and bioelectronic devices.

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Section: Electrochemical Sensing Characteristics Of Tyr-fe 3 O 4 -Chimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of Dopamine Based on Tyrosinase-Fe3O4 Nanoparticles-chitosan Nanocomposite Biosensor

Zhang¹,

Yang²,

Xu³

et al. 2010

Am. J. Biomed. Sci.

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“…Various methods have been described for tyrosinase immobilization on a range of suitable substrates using conventional electrode materials as substrates. These include silver [12], glassy carbon [13,14], graphite-epoxy resin [15], gold [16], boron-doped diamond [17], core-shell magnetic nanoparticles supported on a carbon paste electrode [18], ZnO nanoparticles [19] in addition to other materials [20][21][22][23][24]. Some reports also relate to the electrochemical determination of kinetic parameters of mushroom tyrosinase [25].…”

Section: Introductionmentioning

confidence: 99%

Electrodeposition of nickel oxide nanoparticles on glassy carbon surfaces: application to the direct electron transfer of tyrosinase

et al. 2008

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This work describes the performance of a tyrosinase/nickel oxide nanoparticles/glassy carbon (Tyr/NiO NPs/GC) electrode. This electrode was prepared by first applying a NiO NPs electrochemical deposition onto the GC electrode surface and then tyrosinase immobilization was applied to the surface of electrodeposited NiO NPs. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) procedures demonstrated the existence of different NiO NP geometrical structures. These geometrical structures could lead to better immobilization of proteins on their surfaces. The copper containing enzyme tyrosinase successfully achieved electrical contact with the electrode because of the unique structural alignment of tyrosinase enzyme on the nanometer-scale nickel oxide surfaces. This method could be suitable for application to nanofabricated devices facilitating better performance. It was concluded that tyrosinase can be effectively applied to nanometer-scale nickel oxide surfaces.

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“…Copolymerization does not require rigorous experimental conditions, and can be employed for the polymerization of a large variety of monomers leading to the formation of new advantageous materials (Böyükbayram et al, 2006;Kuwahara et al, 2005;Yilmaz et al, 2004;Yilmaz et al, 2005). Nanomaterials have also been used to improve the operational characteristics of biosensors (Yang et al, 2006;Zhou et al, 2007;Rajesh et al, 2005;Shan et al, 2007). This improvement results from both increased surface area and increased catalytic activity.…”

Section: Introductionmentioning

confidence: 99%

Enzyme based phenol biosensors

Ozoner¹,

Erhan²,

Yılmaz³

2011

Environmental Biosensors

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Amperometric biosensor based on tyrosinase immobilized on a boron-doped diamond electrode

Cited by 113 publications

References 36 publications

Detection of Dopamine Based on Tyrosinase-Fe3O4 Nanoparticles-chitosan Nanocomposite Biosensor

Detection of Dopamine Based on Tyrosinase-Fe3O4 Nanoparticles-chitosan Nanocomposite Biosensor

Electrodeposition of nickel oxide nanoparticles on glassy carbon surfaces: application to the direct electron transfer of tyrosinase

Enzyme based phenol biosensors

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