Amperometric biosensor for microRNA based on the use of tetrahedral DNA nanostructure probes and guanine nanowire amplification

Huang, Yan; Mo, Shi; Gao, Zhong Feng; Chen, Jing Rong; Jing, Lei; Luo, Hong Qun; Li, Nian Bing

doi:10.1007/s00604-017-2246-8

Cited by 47 publications

(18 citation statements)

References 42 publications

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“…In an electrochemical biosensor, the transducer translates biologic events, such as an immune reaction or a nucleic acid hybridization, into an electrical signal. There are representative analytical methods for the detection of biologic events, which are amperometry, potentiometry, voltammetry and impedance spectroscopy [ 73 , 74 ]. Among them, amperometry measures the change of current with constant potential, whereas potentiometric analysis converts biologic events to the equilibrium potential difference.…”

Section: Electrochemical-based Analysis Methods For Ev Detectionmentioning

confidence: 99%

Applications of Bionano Sensor for Extracellular Vesicles Analysis

2020

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Recently, extracellular vesicles (EVs) and their contents have been revealed to play crucial roles in the intrinsic intercellular communications and have received extensive attention as next-generation biomarkers for diagnosis of diseases such as cancers. However, due to the structural nature of the EVs, the precise isolation and characterization are extremely challenging. To this end, tremendous efforts have been made to develop bionano sensors for the precise and sensitive characterization of EVs from a complex biologic fluid. In this review, we will provide a detailed discussion of recently developed bionano sensors in which EVs analysis applications were achieved, typically in optical and electrochemical methods. We believe that the topics discussed in this review will be useful to provide a concise guideline in the development of bionano sensors for EVs monitoring in the future. The development of a novel strategy to monitor various bio/chemical materials from EVs will provide promising information to understand cellular activities in a more precise manner and accelerates research on both cancer and cell-based therapy.

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Section: Electrochemical-based Analysis Methods For Ev Detectionmentioning

confidence: 99%

Applications of Bionano Sensor for Extracellular Vesicles Analysis

2020

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“…The principle of an electrochemical biosensor based on hemin-G-quadruplex as a DNAzyme is presented in Figure 6 C. In this context, hemin-G-quadruplex was employed to catalyze H 2 O 2 reduction, with coupling with HCR to fabricate long hemin-G-quadruplex DNAzyme nanowires (see Figure 11 ) [ 129 ]. In other work, hemin-G-quadruplex was also used for miRNA analysis by catalyzing the oxidation of TMB in the presence of H 2 O 2 [ 130 ].…”

Section: Electrochemical Biosensor Based On Catalystsmentioning

confidence: 99%

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

et al. 2020

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Cancer is the second most fatal disease in the world and an early diagnosis is important for a successful treatment. Thus, it is necessary to develop fast, sensitive, simple, and inexpensive analytical tools for cancer biomarker detection. MicroRNA (miRNA) is an RNA cancer biomarker where the expression level in body fluid is strongly correlated to cancer. Various biosensors involving the detection of miRNA for cancer diagnosis were developed. The present review offers a comprehensive overview of the recent developments in electrochemical biosensor for miRNA cancer marker detection from 2015 to 2020. The review focuses on the approaches to direct miRNA detection based on the electrochemical signal. It includes a RedOx-labeled probe with different designs, RedOx DNA-intercalating agents, various kinds of RedOx catalysts used to produce a signal response, and finally a free RedOx indicator. Furthermore, the advantages and drawbacks of these approaches are highlighted.

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“…The resulting nucleic acid biosensing scaffolds were able to tailor sensitivity according with the required application by the precise control of the spacing between the immobilized probes [82], exhibited higher stability (the tetrahedron moved slower than monothiolated probes [77]), a 5000-fold greater affinity, and were less-prone to unspecific adsorptions than the biosensors constructed with single point-tethered oligonucleotides [83]. The tetrahedral bioscaffolds were used to immobilize DNA probes [77,79,82,83,84,85], antibodies [80,86,87], and aptamers [77,78] as well as in connection with different amplification strategies including hybridization chain reaction (HCR [79]), rolling circle amplification (RCA [88]), analyte-triggered nanoparticle localization-HCR dual amplification [89], DNA tetrahedral nanostructures as reporter probes [85], and guanine nanowire amplification [90]. These low fouling DNA nanostructured bioplatforms were applied to the determination of nucleic acids (DNAs [77,82,83,84] and miRNAs [79,88,89,90,91,92,93]) proteins (TNF-α [86], thrombin [77], and prostate specific antigen (PSA) [80]) and peptides (pneumococcal surface protein A (PspA) peptide [87]) as well as of small molecules (cocaine [78]) in particularly fouling samples such as serum [77,78,80,82,90], PCR products from clinical samples [83], cell lysates [87], and total RNA extracted from cells [88,89], serum [88], and tumor tissues [91,92].…”

Section: Antibiofouling Thiolated Monolayersmentioning

confidence: 99%

Antifouling (Bio)materials for Electrochemical (Bio)sensing

Campuzano

Pedrero

Yáñez‐Sedeño

et al. 2019

IJMS

107

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(Bio)fouling processes arising from nonspecific adsorption of biological materials (mainly proteins but also cells and oligonucleotides), reaction products of neurotransmitters oxidation, and precipitation/polymerization of phenolic compounds, have detrimental effects on reliable electrochemical (bio)sensing of relevant analytes and markers either directly or after prolonged incubation in rich-proteins samples or at extreme pH values. Therefore, the design of antifouling (bio)sensing interfaces capable to minimize these undesired processes is a substantial outstanding challenge in electrochemical biosensing. For this purpose, efficient antifouling strategies involving the use of carbon materials, metallic nanoparticles, catalytic redox couples, nanoporous electrodes, electrochemical activation, and (bio)materials have been proposed so far. In this article, biomaterial-based strategies involving polymers, hydrogels, peptides, and thiolated self-assembled monolayers are reviewed and critically discussed. The reported strategies have been shown to be successful to overcome (bio)fouling in a diverse range of relevant practical applications. We highlight recent examples for the reliable sensing of particularly fouling analytes and direct/continuous operation in complex biofluids or harsh environments. Opportunities, unmet challenges, and future prospects in this field are also pointed out.

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Amperometric biosensor for microRNA based on the use of tetrahedral DNA nanostructure probes and guanine nanowire amplification

Cited by 47 publications

References 42 publications

Applications of Bionano Sensor for Extracellular Vesicles Analysis

Applications of Bionano Sensor for Extracellular Vesicles Analysis

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

Antifouling (Bio)materials for Electrochemical (Bio)sensing

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