Amperometric biosensors for detection of L‐ and D‐amino acids based on coimmobilized peroxidase and L‐ and D‐amino acid oxidases in carbon paste electrodes

Kacaniklic, V.; Johansson, K.; Markó-Varga, György; Gorton, Lo; Jönsson–Pettersson, G.; Csöregi, Elisabeth

doi:10.1002/elan.1140060505

Cited by 98 publications

(39 citation statements)

References 38 publications

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“…Since the oxidase step involves biological recognition and not electrical transduction, it was considered plausible that G2 would contribute with beneficial activating effects on P2O, but not to the background current. The graphite powder (from Fluka) used in most of the previous publications concerning carbon paste from our laboratory [12,[15][16][17]19], including this work, was shown to give rise to medium sensitivity and background current [41], and was thus used as a comparison to the graphite powders mentioned above. The responses to glucose and hydrogen peroxide as well as the observed background currents are listed in Table 2.…”

Section: Graphitesmentioning

confidence: 99%

Pyranose Oxidase Modified Carbon Paste Electrodes for Monosaccharide Determination

et al. 1998

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An amperometric biosensor for the detection of some monosaccharides was developed with co-immobilized pyranose oxidase (purified from the basidiomycete fungus Phanerochaete chrysosporium) and horseradish peroxidase in carbon paste. Due to rather low selectivity of pyranose oxidase, the biosensor could detect both predominating anomers of D-glucose as well as the substrates D-xylose, D-galactose, and d-gluconolactone. The best sensor performance was obtained from a carbon paste with covalently immobilized enzymes together with the additives lactitol and polyethylenimine, resulting in a sensitivity for glucose of 30.2 mA cm ¹2 mM ¹1 . The enzyme modified electrode was investigated in an automated flow injection system at the operating potential of ¹50 mV (vs. Ag/AgCl) with an injection frequency of 40 h ¹1 . The biosensor was also integrated as the detection unit in a liquid chromatographic system for the detection of monosaccharides to show its potential use in real applications.

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Section: Graphitesmentioning

confidence: 99%

Pyranose Oxidase Modified Carbon Paste Electrodes for Monosaccharide Determination

et al. 1998

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“…Gorton et al [18,19] proposed immobilization of an enzyme in carbon paste electrodes for enantioselective analysis of L-and D-amino acids. This construction is more reliable than those in which plastic membranes (PVC matrix) are used for enzyme immobilization, due to the reproducible repartition of the enzyme at the sensor surface.…”

Section: The Design Of Amperometric Biosensorsmentioning

confidence: 99%

Analysis of Chiral Drugs with Enantioselective Biosensors. An Overview

Stefan¹,

Staden

Aboul‐Enein

1999

Electroanalysis

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The opportunity to use amperometric biosensors based on L-aminoacid oxidase in enantioselective analysis of chiral drugs is discussed. The designed biosensors combine the selectivity of the enzyme with the sensitivity of the amperometric device. To obtain a reliable amperometric biosensor, graphite paste is used as support for the enzyme. The parameters obtained for amperometric biosensors are compared with those obtained for the potentiometric enantioselective membrane electrodes based on b-cyclodextrin derivates impregnated in graphite paste for several angiotensin converting enzyme (ACE) inhibitors ± S-captopril, S-enalapril, S-ramipril, S-cilazapril, S-trandolapril, S-pentopril, and S-perindopril ± in terms of sensitivity, selectivity, limit of detection, working concentration range, lifetime, response time, accuracy and precision.

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“…D-AAOx or LAAOx were involved to the construction of biosensor sensitive to D or L amino acids. Value of the signal obtained for over 20 common L-amino acids and six important D-amino acids were presented in (Sarkar et al 1999) and compared with the results obtained in (Kacaniklic et al 1994). In the first case an enzyme was immobilized on the screen-printed electrode with the addition of polyethylamine (PEI) to the working electrode paste.…”

Section: Chiral Catalysismentioning

confidence: 99%