Amperometric Detection and Quantification of Nitrate Ions Using a Highly Sensitive Nanostructured Membrane Electrocodeposited Biosensor Array

Gokhale, Ankush A.; Lu, Jue; Weerasiri, R. R.; Yu, Jing; Lee, Ilsoon

doi:10.1002/elan.201400547

Cited by 35 publications

(12 citation statements)

References 62 publications

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“…The operational stability and reproducibility of the GCE/ RGO/PPy/NR biosensor were tested by the repeated experiments under similar conditions. The lowest concentration of the nitrate ions, which can be detected under the laboratory conditions, i.e., the limit of detection (LOD), has been evaluated by using the following equation (Gokhale et al 2015): where, SD and m represent the standard deviation and slope of the calibration curve. The value so obtained for the present biosensor is compared with that reported for the different nitrate biosensors by other investigators in Table 1.…”

Section: Working Stability and Reproducibility Of The Biosensormentioning

confidence: 99%

Reduced graphene oxide/polypyrrole/nitrate reductase deposited glassy carbon electrode (GCE/RGO/PPy/NR): biosensor for the detection of nitrate in wastewater

Umar

Nasar

2018

Appl Water Sci

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In the present work, a novel biosensor (GCE/RGO/PPy/NR) based on the nanocomposite of reduced graphene oxide (RGO), polypyrrole (PPy) immobilized by nitrate reductase (NR) was developed on a glassy carbon electrode (GCE). The conductive nanocomposite (RGO/PPy) was synthesized by in situ oxidative polymerization of pyrrole in the presence of RGO in acidic medium. A facile and green path was employed to synthesize RGO from graphene oxide (GO). This was performed by a novel route using Abelmoschus esculentus vegetable extract as a stabilizing and reducing agent for GO. The composite of reduced graphene oxide and polypyrrole (RGO/PPy) was deposited onto GCE with subsequent deposition of NR enzyme on the GCE/ RGO/PPy to develop GCE/RGO/PPy/NR biosensor. The surface morphology and structural features of the composites were studied by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The electrochemical behavior and electrocatalytic activity of the biosensor were examined by cyclic voltammetry at different scan rates (20-100 mV s −1) in the synthetic nitrate solution. The developed bio-anode achieved a maximum current density of 4.24 mA cm −2 at a scan rate of 100 mV s −1 for 10 mM sodium nitrate solution.

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Section: Working Stability and Reproducibility Of The Biosensormentioning

confidence: 99%

Reduced graphene oxide/polypyrrole/nitrate reductase deposited glassy carbon electrode (GCE/RGO/PPy/NR): biosensor for the detection of nitrate in wastewater

Umar

Nasar

2018

Appl Water Sci

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“…Water pollution represents a risk to the human and environment. According to the United States Environmental Protection Agency, the standard limit for nitrate concentration in drinking water is about 44 mg NO 3 − L −1 (Gokhale, Lu, Weerasiri, Yu, & Lee, ; Kaviani et al, ).…”

Section: Introductionmentioning

confidence: 99%

Nitrate removal from aqueous solutions by adsorption onto hydrogel‐rice husk biochar composite

Afjeh

Marandi

Zohuriaan‐Mehr

2020

Water Environment Research

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In the present study, we investigated the performance of hydrogel-rice husk biochar composites, as low-cost, alternative, and biocompatible adsorbents for separating nitrate ions from aqueous solutions. Hydrogel-biochar composite was synthesized at dosages of 2.5%, 3.6%, 4.8%, and 9.6% weight ratios of biochar. The composite was characterized by several common methods including FTIR, SEM, TEM, TGA, and DSC. In addition, the effect of contact time, initial concentration of nitrate ions, and solution pH were considered. The maximum removal of nitrate was about 34.3% at acidic pH (pH = 3) using 0.02 g of adsorbent in 25 ml of nitrate solution with the initial concentration (20 mg/L) and temperature of 25°C for 60 min. Based on the findings, 5% biochar in the composite was the optimal dosage. Adsorption kinetic study revealed that this process followed the first-order kinetic model. The experimental equilibrium adsorption data were tested by the Temkin isotherm model with R 2 > 0.97. Based on the thermodynamic studies, the adsorption process was endothermic and spontaneous. Overall, the results suggested that the obtained composite can be specifically employed for removal of contaminations from aqueous solutions. © 2020 Water Environment Federation • Practitioner points• Hydrogel-biochar composite provides a biocompatible and cost-effective adsorbent. • Hydrogel-biochar composite was applied to eliminate nitrate from aqueous solutions. • Nitrate removal increased in the synthesized composite upon elevation of the weight ratio of biochar to 0.2 g.

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“…Therefore, there is an urgent need to develop sensitive, miniature, and low cost nitrate sensors. [3][4][5][6][7][8] Electrochemical sensors often use biochemical receptors to recognize specific target molecules and produce electrical signals by catalytic reaction. 9 With high sensitivity, sign-to-noise ratio, and selectivity, they have played an important role for real-time measurements of biochemical species in many applications.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic impedimetric sensor for soil nitrate detection using graphene oxide and conductive nanofibers enabled sensing interface

Ali

Jiang

Mahal

et al. 2017

Sensors and Actuators B: Chemical

127

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This paper reports on a microfluidic Impedimetric nitrate sensor using graphene oxide (GO) nanosheets enabled poly(3,4-ethylenedioxythiophene) nanofibers (PEDOT-NFs) as an electrochemical working electrode. The sensor has demonstrated its capability to detect nitrate ions in real samples extracted from soil. The PEDOT NFs-GO composite serves as an effective matrix for immobilization of nitrate reductase enzyme molecules. The oxygenated functional groups available at GO allows increasing the charge transfer resistance of the PEDOT NFs-GO based electrode. Systematic microscopic, spectroscopic, and electrochemical studies were conducted to illustrate synergic interactions between the GO and PEDOT NFs. The sensor provides a sensitivity of 61.15 Ω/(mg/L)/cm 2 within a concentration range of 0.44 − 442 mg/L for nitrate ions. The detection limit of the sensor is 0.135 mg/L with good specificity, reliability, and reproducibility.

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Amperometric Detection and Quantification of Nitrate Ions Using a Highly Sensitive Nanostructured Membrane Electrocodeposited Biosensor Array

Cited by 35 publications

References 62 publications

Reduced graphene oxide/polypyrrole/nitrate reductase deposited glassy carbon electrode (GCE/RGO/PPy/NR): biosensor for the detection of nitrate in wastewater

Reduced graphene oxide/polypyrrole/nitrate reductase deposited glassy carbon electrode (GCE/RGO/PPy/NR): biosensor for the detection of nitrate in wastewater

Nitrate removal from aqueous solutions by adsorption onto hydrogel‐rice husk biochar composite

Microfluidic impedimetric sensor for soil nitrate detection using graphene oxide and conductive nanofibers enabled sensing interface

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