Amperometric detection of Enterobacteriaceae in river water by measuring β-galactosidase activity at interdigitated microelectrode arrays

Laczka, Olivier; García-Aljaro, Cristina; Campo, F. Javier del; Pascual, Francesc Xavier Muñoz; Mas, Jordi; Baldrich, Eva

doi:10.1016/j.aca.2010.08.001

Cited by 47 publications

(38 citation statements)

References 44 publications

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“…Specifically designed IMEs have been used for detection of single cells (1-10 μm) (Huang and Rubinsky 2001) or a population of cells (10-500 μm) (Yang et al 2004). Besides their use in impedance measurements, the IME electrodes have also been extensively used for generator-collector electrochemistry in the detection of chemicals and biomolecules (Iwasaki and Morita 1995;Senior et al 2001;Tomcik et al 2006;Laczka et al 2010). The co-planar IMEs offer ease of fabrication, ready integration in electronic devices, the potential for multi-analyte sensing through the fabrication of arrays, and higher sensitivities than conventional electrodes.…”

Section: Introductionmentioning

confidence: 98%

Interdigitated microelectrode-based microchip for electrical impedance spectroscopic study of oral cancer cells

Mamouni

Yang

2011

Biomed Microdevices

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In this study, electric/electrochemical impedance spectroscopy and cyclic voltammetry were used to study the cellular activities of oral cancer cell line CAL 27, including the kinetics of cell adhesion, spreading, and cell proliferation on interdigitated microelectrodes (IMEs). Impedance spectra of CAL 27 cells on IMEs electrodes were obtained in cell growth medium and in 0.1 M PBS with 50 mM [Fe(CN)₆]³⁻/⁴⁻ as redox probe. Equivalent circuits were used to model both cases. In cell growth medium, impedance spectra allowed us to analyze the changes in capacitance and resistance due to cell attachment on the IMEs over the entire experiment period. It was found that cell spreading caused the most significant decrease in capacitance component and slight increase in resistance component. Impedance change at given frequencies (between 10 kHz to 100 kHz) was found to be linearly increased with increasing cell number of CAL 27 on the IMEs. In comparison with non-cancer oral epithelial cells (Het-1A), at equal cell number, cancer cells always generated impedance several folds higher than that of non-cancer cells. In the presence of [Fe(CN)₆]³⁻/⁴⁻, impedance spectra allowed us to analyze the change in electron transfer resistance of IMEs due to cell attachment, in which an increase trend was observed at 24 h with increasing cell number from 2500 cells to 10,000 cells on IMEs. Double layer capacitance was also affected by cell attachment, and a decrease in double layer capacitance was observed with increasing cell number on the electrodes. Cyclic voltammetric measurements correlated well with the impedance results. The results of this study demonstrated the use of electrochemical approaches to obtain and understand cellular behaviors/activities of oral cancer cells, potentially providing useful tools for cancer cell research.

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Section: Introductionmentioning

confidence: 98%

Interdigitated microelectrode-based microchip for electrical impedance spectroscopic study of oral cancer cells

Mamouni

Yang

2011

Biomed Microdevices

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“…Thus, these changes can be measured and 28 related to bacterial concentration for determination of microbial 29 growth [3]. Since bacterial metabolism may significantly alter 30 electrical conductivity of the growth media, impedance technique 31 can be successfully applied for estimation of microbial biomass [4], 32 detection of microbial metabolism, as well as for determination of 33 the physiological state of bacteria growth [5][6][7]. Advantages of this 34 approach are high sensitivity, relative simplicity and comparative- 35 ly low cost of the required experimental equipment [8].…”

Section: Q2mentioning

confidence: 99%

Fast determination of viable bacterial cells in milk samples using impedimetric sensor and a novel calibration method

Uría

Moral‐Vico

Abramova

et al. 2016

Electrochimica Acta

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A B S T R A C TAs a first stage toward the development of an impedimetric biosensor to quantify viable bacterial cells, this work defines the relationships between microbial growth and impedance changes associated with acidification of the culture medium. Escherichia coli bacterium was used as a model of a common pathogenic bacterium found in a foodstuff. Impedance was measured at a fix frequency of 10 kHz using interdigitated microelectrodes. Impedance changes were found to depend on the number of microorganisms in a sample. However, as the kinetics greatly depends on the bacterial concentration, it was not possible to obtain a single calibration curve in a wide concentration range at a fixed incubation time. To resolve this, a novel calibration method was proposed by measuring the sensor response at 270 and 390 min of incubation and taking a mean value. Obtained calibration plots were used to determine E. coli in a spiked milk samples. Performed experiments demonstrated the capacity of the method to detect E.coli concentrations in a range between 10 2 and 10 6 cfu mL À1 in milk in only 6 hours with 5-12% error margin.

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“…In addition, another GFP was linked to the phage's outer capsid protein to yield stronger fluorescent emissions. These phages were able to infect approximately 50% of E. coli sewage isolates within a timeframe of 6 h. Moving away from reporter gene constructs, Laczka et al [80] used phage PhiX174 in a phage-mediated cell lysis protocol, assaying for β-galactosidase amperometrically on gold microelectrodes, to detect E. coli in river water at 10 5 CFU mL -1 in 2 h. Using an electrochemical impedance spectroscopy biosensor format, Mejri et al [81] attached T4 phages onto gold microelectrodes using physisorption and demonstrated detection of E. coli at 10 4 CFU mL -1 in 1 h in store-purchased, artificially contaminated mineral water. Using phage PP01 with specificity towards E. coli O157: H7, Ripp et al [63] applied their previously described luxI/ luxR bioluminescent phage reporter system to detect down to 1 CFU E. coli O157:H7 mL -1 in tap water in 12.5 h.…”

Section: Detection Of Pathogens In Processed Waters and Wastewatersmentioning

confidence: 97%

Pathogen detection using engineered bacteriophages

Smartt

Jegier

et al. 2011

Anal Bioanal Chem

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Bacteriophages, or phages, are bacterial viruses that can infect a broad or narrow range of host organisms. Knowing the host range of a phage allows it to be exploited in targeting various pathogens. Applying phages for the identification of microorganisms related to food and waterborne pathogens and pathogens of clinical significance to humans and animals has a long history, and there has to some extent been a recent revival in these applications as phages have become more extensively integrated into novel detection, identification, and monitoring technologies. Biotechnological and genetic engineering strategies applied to phages are responsible for some of these new methods, but even natural unmodified phages are widely applicable when paired with appropriate innovative detector platforms. This review highlights the use of phages as pathogen detector interfaces to provide the reader with an up-to-date inventory of phage-based biodetection strategies.

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Amperometric detection of Enterobacteriaceae in river water by measuring β-galactosidase activity at interdigitated microelectrode arrays

Cited by 47 publications

References 44 publications

Interdigitated microelectrode-based microchip for electrical impedance spectroscopic study of oral cancer cells

Interdigitated microelectrode-based microchip for electrical impedance spectroscopic study of oral cancer cells

Fast determination of viable bacterial cells in milk samples using impedimetric sensor and a novel calibration method

Pathogen detection using engineered bacteriophages

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