Amperometric Detection of Peroxides Using Peroxidase and Porphyrin Biomimetic Modified Electrodes

Peroxidase Mimetic Activity at Tailored Nanocarbon Electrodes

“…(Though it may seem as though the most sensitive response should occur at the apex of the ORR peak, previous H 2 O 2 -based RDE amperometry studies using enzyme-modified electrodes have reported optimal H 2 O 2 detection at potentials near the onset of the enzyme's redox peak, as well (12,30).) As evident in Figure 1b As mentioned in the Introduction, several research groups have reported "peroxidase mimetic" activity of several Fe-based species (18)(19)(20)31 , thus regenerating the native enzyme (H 2 O is formed from the ferryl oxygen atom). Though the above reactions have been written in the most simplified manner possible, it should be noted that Reactions 5 and 6 also involve proton transfer from AH 2 to E1 and E2 (32)(33)(34).…”

“…Additionally, small redox molecules such as ferrocenes have been used to mediate glucose and glutamate detection (4,17). Alternatively, the "peroxidase mimetic" activity of microperoxidases and Fe protoporphyrin IX in electrode assemblies has been reported (18,19). These "miniature versions" of HRP, which are capable of reducing H 2 O 2 to H 2 O through a 2-electron, Fe-mediated process, may exchange electrons more efficiently than HRP in electrochemical biosensing schemes due to the lack of protein residues surrounding their active sites.…”

Metalloporphyrin electropolymerization: electrochemical quartz crystal microgravimetric studies

“…Model systems that mimic enzymes [25] require more than one component with different functions, like enzymes and redox proteins with one prosthetic group acting as a catalyst and another one as the electron transfer unit [26]. We now describe a two-step electrochemical polymerization of FePP and CuPP in either FePP/CuPP or CuPP/FePP sequence and investigate the behavior of the resulting films at electrodes in terms of QCM microgravimetric studies.…”

“…These EP rate studies with EQCM have determined the optimal conditions for the growth of duplex electroactive M 1 PP/M 2 PP films, which have interesting analytical applications [25]. …”

Smart organometallic polymer platforms for redox sensing and as metal nanopatricle foundry

“…Much effort has been made to enhance the conductivity and performance of osmium-polymer-hydrogel-based biosensors. 115,[127][128][129][130][131] For example, new linkers were introduced between the osmium complex and the polymer backbone, 132 co-electrodeposition techniques were employed to form crosslinked thin films from enzymes and polymers, 133 and carbon nanotubes or graphene were integrated with the polymers. 134 Zafar et al assembled FAD-dependent, glucose dehydrogenase (GcGDH) based hydrogel thin films with different Os polymers on graphite electrodes for glucose sensing.…”