Amperometric Enzyme-Free Glucose Sensor Based on Electrodeposition of Au Particles on Polyaniline Film Modified Pt Electrode

Abstract

An amperometric non-enzymatic glucose sensor of high sensitivity was developed by modification of Pt electrode with electrodeposition of Au particles on polyaniline film. The Au particles were deposited under optimum growth conditions for maximum dispersion on polymer film to achieve highly sensitive glucose sensor. The characterization of the electrode and the analytical parameters were evaluated using chronoamperometry and cyclic voltammetry techniques. The sensor showed wide linear range from 0.01-8 mM along with swift response time of less than 5 s. Moreover, a high sensitivity of 113.6 µA mM-1 cm-2 is obtained along with low detection limits of 0.5 µM. The sensor also showed high stability, reproducibility, and repeatability. Furthermore, the sensor has shown high selectivity towards glucose exclusively in the presence of interferents such as sucrose, uric acid, and ascorbic acid. The practical applicability of the proposed sensor was confirmed from successful analysis of glucose in different juice samples and these results were comparable to the commercial glucose meter. Thus, our sensor stands promising non-enzymatic sensor for routine analysis of glucose.

Non-enzymatic screen-printed sensor based on PtNPs@polyazure A for the real-time tracking of the H2O2 secreted from living plant cells

“…A clear oxidation peak appeared at 0.58V and 0.56V for the aSPCE and PAA(DS)/aSPCE, respectively, in the presence of H2O2, which shifted to a lower anodic potential (0.3V) when PtNPs were electrodeposited on the conjugated polymer. Peak intensity sharply increased on Pt@PAA(DS)/aSPCE, which indicated that the synergistic effect of the conjugated polymer and metal nanoparticles improved electrocatalytic activity [25,37,38]. These results demonstrated that Pt@PAA(DS)/aSPCEs possess outstanding electrocatalytic activity toward H2O2 oxidation and high sensitivity, which suggests that they can be used for the real-time monitoring of oxidative stress in living cell suspensions.…”

Determination of Vitamin C at Modified Screen Printed Electrode: Application for Sensing of Vitamin C in Real Samples

“…Electrochemical methods, especially voltammetry, have attracted researchers interest in recent years as it offers a more feasible, cost-effective, and selective determination of the environmental, clinical, and biotechnical targets [1][2][3][4][5][6].…”

Amperometric Biosensors for Glucose and Lactate with Applications in Food Analysis: A Brief Review

“…In food quality control, the development of reliable, sensitive and selective methods for fast, precise sensing and quantification of food ingredients and supplements, [1][2][3][4] toxicants, [5][6][7][8][9] antibiotics, and allergens, 10 is an issue of constantly increasing importance. [11][12][13][14][15][16] Recently, as a new direction of the analytical technology is distinguished the development of electrochemical enzymatic biosensorscomplex systems that include an immobilised enzyme (bioelement) and a physical transducer of the signal which may be potentiometric or amperometric, as well as a device for signal reading and processing.…”