Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles

Abstract

GOD was immobilized onto polypyrrole (PPy) or poly(o-anisidine) (POA) coated Pt electrode to construct glucose sensitive biosensor. Because polymer film properties and enzyme activity affect the current response, PPy and POA synthesis conditions and also enzyme immobilization parameters were optimized in detail. The optimal monomer concentrations were determined as 25 and 50 mM for PPy and POA, respectively, whereas scan rate was 50 mV/s for both polymer films. In case of immobilization procedure, the optimal Chitosan (Chi), glucose oxidase (GOD) and glutaraldehyde (GAL) concentrations were determined as 0.5%, 2 mg/ml and 0.05% for PPy and 0.5%, 4 mg/ml and 0.075% for POA, respectively. Zinc oxide nanoparticles (ZnONP) were co-immobilized with GOD enzyme and it was revealed that ZnONP modification enhanced the efficiencies of both electrodes in terms of current responses and stabilities. Nyquist diagrams showed that enzyme electrodes were sensitive to glucose molecule and ZnONP modification improved the sensor efficiency.

Response surface methodology use in construction of polianiline‐coated carbon paste electrode–based biosensor: Modification and characterization

“…[13][14][15][16] Glucose oxidase (GOD), in addition to being used in the first biosensor design, 17 also shows the feature of being the most used enzyme in glucose biosensor construction. 4,[18][19][20][21][22] PANI has received a lot of attention in biosensor applications due to its flexibility in changing its chemical structure, its compatibility with biological molecules, and its ability to efficiently transfer electrical charges produced as a result of biochemical reactions to electronic circuits. 23 Among the conductive polymers, the biosensor applications of PANI have an important place.…”

Optimization of Graphite-Mineral Oil Ratio With Response Surface Methodology in Glucose Oxidase Based Carbon Paste Electrode Design

“…Therefore, enzyme immobilization is an important step in biosensor design. The first and most widely used enzyme used in biosensor design is glucose oxidase (Luque et al, 2005;Li & Lin, 2007;Comba et al, 2010;Ozyilmaz et al, 2011;Ozyilmaz et al, 2018;Ozyilmaz et al, 2019).…”

Chitosan-silica Sulfate Nano Hybrid: An Efficient Biopolymer Based-heterogeneous Nano Catalyst for Solvent-free Synthesis of 3,4-Dihydropyrimidine-2(1H)-one/thiones

“…34 Accordingly, chitosan has found considerable research interest in numerous fields of science and industry. [35][36][37][38] Recently, we have reported the synthesis, characterization, and application of chitosan-silica sulfate nanohybrid (CSSNH) as a new, green, highly proficient, and cheap heterogeneous nano-catalyst (Figure 2). The high efficacy and potency of CSSNH was confirmed by its successful applications in many organic transformations.…”