Amperometric glucose biosensor based on layer-by-layer assembly of multilayer films composed of chitosan, gold nanoparticles and glucose oxidase modified Pt electrode

Wu, Billy; Hou, Shifeng; Yin, Feng; Li, Jing; Zhao, Zhongwei; Huang, Jiadong; Chen, Qiang

doi:10.1016/j.bios.2006.03.009

Cited by 184 publications

(46 citation statements)

References 53 publications

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“…20 In addition, Wang, et al reported that the permselective properties of Nafion are not sufficient to fully eliminate anionic interferences, especially at high potential. 8 For these reasons, the interfering effects of 0.1 mM ascorbic acid and 0.2 mM uric acid compared to 4 mM glucose (the average physiological concentration of blood glucose 37 ) were evaluated on the PtϪxGnP glucose biosensor at the potential of ϩ0.7 mV. Addition of 0.1 mM AA or 0.2 mM UA to a 4 mM glucose solution resulted in a 8.0 or 18% increase in the biosensor's output, respectively.…”

Section: Resultsmentioning

confidence: 99%

Nanometal-Decorated Exfoliated Graphite Nanoplatelet Based Glucose Biosensors with High Sensitivity and Fast Response

et al. 2008

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We report the novel fabrication of a highly sensitive, selective, fast responding, and affordable amperometric glucose biosensor using exfoliated graphite nanoplatelets (xGnPs) decorated with Pt and Pd nanoparticles. Nafion was used to solublize metal-decorated graphite nanoplatelets, and a simple cast method with high content organic solvent (85 wt %) was used to prepare the biosensors. The addition of precious metal nanoparticles such as platinum (Pt) and palladium (Pd) to xGnP increased the electroactive area of the electrode and substantially decreased the overpotential in the detection of hydrogen peroxide. The Pt−xGnP glucose biosensor had a sensitivity of 61.5 ± 0.6 μA/(mM·cm2) and gave a linear response up to 20 mM. The response time and detection limit (S/N = 3) were determined to be 2 s and 1 μM, respectively. Therefore, this novel glucose biosensor based on the Pt nanoparticle coated xGnP is among the best reported to date in both sensing performance and production cost. In addition, the effects of metal nanoparticle loading and the particle size on the biosensor performance were systematically investigated.

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Section: Resultsmentioning

confidence: 99%

Nanometal-Decorated Exfoliated Graphite Nanoplatelet Based Glucose Biosensors with High Sensitivity and Fast Response

et al. 2008

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“…However, the direct electrocatalysis of H 2 O 2 requires a high working potential. At such potential, the electrochemically active interfering species are easily oxidized, which leads to interfering current [17]. Prussian blue (PB), as a mediator, is an excellent catalyst for H 2 O 2 reduction at low potentials, which greatly decreases the interference [18].…”

Section: Introductionmentioning

confidence: 99%

Glucose biosensor based on titanium dioxide-multiwall carbon nanotubes-chitosan composite and functionalized gold nanoparticles

Zhang

Yuan

et al. 2011

Bioprocess Biosyst Eng

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In this paper, a new glucose biosensor was prepared. At first, Prussian blue (PB) was electrodeposited on a glassy carbon electrode (GCE) modified by titanium dioxide-multiwall carbon nanotubes-chitosan (TiO(2)-MWNTs-CS) composite, and then gold nanoparticles functionalized by poly(diallyldimethylammonium chloride) (PDDA-Au) were adsorbed on the PB film. Finally, the negatively charged glucose oxidase (GOD) was self-assembled on to the positively charged PDDA-Au. The electrochemical performances of the modified electrodes had been studied by cyclic voltammetry (CV) and amperometric methods, respectively. In addition, the stepwise fabrication process of the as-prepared biosensor was characterized by scanning electron microscopy. PDDA-Au nanoparticles were characterized by ultraviolet-vis absorption spectroscopy and transmission electron microscopy. Under the optimal conditions, the as-prepared biosensor exhibited a good response performance to glucose with a linear range from 6 μM to 1.2 mM with a detection limit of 0.1 μM glucose (S/N = 3). In addition, this work indicated that TiO(2)-MWNTs-CS composite and PDDA-Au nanoparticles held great potential for constructing biosensors.

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“…20,21 Our group has reported extensively on LbL based single and bi-enzyme biosensing systems incorporating multi-walled carbon nanotubes (MWCNTs) immobilized with organophosphorus hydrolase (OPH) and acetylcholinesterase (AChE), for discriminative detection between organophosphorus and non-organophosphorus pesticides. 13,22 In these organized LbL nanostructures, a homogenous and stable CNT-based assembly of multi-enzyme interfaces with desired architecture provides control over the position of the polyelectrolyte and the enzyme molecules compared to random hydrogels.…”

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confidence: 99%

Layer-by-Layer Assembly of Carbon Nanotubes Modified with Invertase/Glucose Dehydrogenase Cascade for Sucrose/O₂Biofuel Cell

Zhang

Arugula

Williams

et al. 2016

J. Electrochem. Soc.

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A layer-by-layer (LbL) assembly technique was employed to modify glassy carbon electrodes (GCEs) and screen printed electrodes (SPEs) utilizing multiwalled carbon nanotubes (MWCNTs)/polyelectrolyte binary composites and an enzyme cascade to facilitate efficient electron transfer in sucrose/O 2 biofuel cell. In this study, MWCNTs immobilized invertase (INV) and glucose dehydrogenase (GDH) were alternatively assembled upon polyethyleneimine (PEI) and DNA nanocomposites to construct a bioanode.[Ni(phendion)(phen)]Cl 2 complex and methylene green (MG) were investigated as redox mediators for electrocatalytic oxidation of NADH at reduced overpotentials. The LbL architecture showed advantages for sequential enzymatic reaction that favored the efficient penetration of substrate and products in a cascade system while MWCNTs facilitated the efficient electron transfer from sucrose oxidation and enhancement of the current density. With a GCE bioanode modified with a MG or Ni complex, the biofuel cell produced a higher power density (μW/cm 2 ) with 145.8% and 130.11% enhancement comparing to a SPE bioanode, respectively. Moreover, the Ni complex modified GCEs and SPEs produced power densities (μW/cm 2 ) 93.7% and 107.0% higher compared to MG modified bioanodes, respectively. The maximum current density of 1400 ± 46 μA/cm 2 was obtained with the Ni complex on GCE at an OCP of 604 ± 17 mV with a maximum power density of 405 ± 6 μW/cm 2 . The LbL assembly showed great feasibility as a simple and efficient way to construct controlled MWCNT-multi-enzyme modified electrodes for biofuel cell applications. Biofuel cells convert the chemical energy of biofuels into electric energy via the enzymatically catalyzed oxidation and reduction reactions. One of the most important factors affecting the performance of fuel cell is the fabrication of bioanode which has great influence in generation of electrical power outputs. Therefore developing effective bioanodes remains critical. Till date, several reports have been published on various approaches to assemble enzymes on the anodes in BFCs utilizing carbon based materials.1-4 Amongst those layerby-layer (LbL) assembly technique via electrostatic interactions of oppositely charged species has emerged as a very attractive way to construct multilayer films of polyelectrolytes, biomolecules, 5 and organic materials 6-8 offering advantages in various fields, such as surface functionalization, 9,10 drug delivery, 11,12 and biosensing. [13][14][15] Its application was considered plausible in the development of amperometric biosensors initiating vast research activities on biosensors comprised of LbL organized multilayers. LbL nanostructures decorated with multi-enzymes were proven to be one of the successful strategies to establish high electrical performance, long-term stability and long lifetime in bioelectronics devices.14,16-18 For example, LbL structures consisting of Au nanoparticles (AuNPs), thiol-functionalized polyaniline and glucose oxidase (GOx) were fabricated for glucose biosensing ...

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Amperometric glucose biosensor based on layer-by-layer assembly of multilayer films composed of chitosan, gold nanoparticles and glucose oxidase modified Pt electrode

Cited by 184 publications

References 53 publications

Nanometal-Decorated Exfoliated Graphite Nanoplatelet Based Glucose Biosensors with High Sensitivity and Fast Response

Nanometal-Decorated Exfoliated Graphite Nanoplatelet Based Glucose Biosensors with High Sensitivity and Fast Response

Glucose biosensor based on titanium dioxide-multiwall carbon nanotubes-chitosan composite and functionalized gold nanoparticles

Layer-by-Layer Assembly of Carbon Nanotubes Modified with Invertase/Glucose Dehydrogenase Cascade for Sucrose/O₂Biofuel Cell

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Amperometric glucose biosensor based on layer-by-layer assembly of multilayer films composed of chitosan, gold nanoparticles and glucose oxidase modified Pt electrode

Cited by 184 publications

References 53 publications

Nanometal-Decorated Exfoliated Graphite Nanoplatelet Based Glucose Biosensors with High Sensitivity and Fast Response

Nanometal-Decorated Exfoliated Graphite Nanoplatelet Based Glucose Biosensors with High Sensitivity and Fast Response

Glucose biosensor based on titanium dioxide-multiwall carbon nanotubes-chitosan composite and functionalized gold nanoparticles

Layer-by-Layer Assembly of Carbon Nanotubes Modified with Invertase/Glucose Dehydrogenase Cascade for Sucrose/O2Biofuel Cell

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Layer-by-Layer Assembly of Carbon Nanotubes Modified with Invertase/Glucose Dehydrogenase Cascade for Sucrose/O₂Biofuel Cell