Amperometric glucose sensor based on glucose oxidase immobilized on gelatin-multiwalled carbon nanotube modified glassy carbon electrode

Periasamy, Arun Prakash; Chang, Yu‐Jung; Chen, Shen‐Ming

doi:10.1016/j.bioelechem.2010.06.009

Cited by 191 publications

(96 citation statements)

References 35 publications

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“…This work reports a bioelectrode containing glucose oxidase 45 (GOd) and MWCNT that exhibits direct electron transfer (DET) with a considerably enhanced current output and enzyme turnover compared to previously reported GOd-based bioelectrodes that employ nano-gold, nano-platinum or carbon nanotubes [10][11][12] . The methodology presented yields a low cost enzymatic biofuel cell 50 anode configuration that retains high efficiency DET between the enzyme and the electrode.…”

Section: Discussionmentioning

confidence: 99%

An optimised glucose oxidase bioelectrode exhibiting high performance direct electron transfer

Milton

Baur

Varcoe

et al. 2012

Phys. Chem. Chem. Phys.

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A glucose oxidase (GOd) bioelectrode exhibiting high performance, direct electron transfer (DET) has been prepared. Unprecedented redox peak current densities of 1 mA cm -2 were observed alongside a clear electrochemical response to glucose. This system shows potential as a low cost, 10 high performance enzymatic bioelectrode.Glucose oxidase (GOd; EC 1.1.3.4), a flavo-protein (molecular weight of ca. 160 kDa) contains two flavin adenine dinucleotide (FAD) redox centres located approximately 13 Å into the protein 15 structure 1 . Typically, electron transfer from the FAD site to the electrode surface is restricted by the protein structure and electrochemical mediators are often used to transport electrons from the FAD sites to the electrode surface on oxidation of glucose (mediated electron transfer, MET) 2 . Direct electron 20 transfer (DET) from the FAD redox centres of the enzyme to the electrode is facilitated by using advanced materials such as graphene and carbon nanotubes 1,[3][4][5] . DET by enzymes employed in bioelectronic devices has recently gained worldwide attention, as there are many advantages with DET in biofuel cells and 25 biosensors (as opposed to MET), such as the ability to reduce the size, toxicity and loss of potential difference in these devices 6 . Enzyme immobilization is essential for high performance and stability. The use of natural biocompatible polymers, such as cellulose 5 and chitosan 7,8 , for the immobilisation of enzymes 30 onto electrode surfaces facilitates low cost and simple electrode preparation. Previous work has shown the combination of multiwalled carbon nanotubes (MWCNT) and cellulose can provide a platform for DET of GOd 5 , and also provide electronically conductive pathways through the cellulose. 35In this study, 4 different methods of bioelectrode preparation were investigated with the aim of achieving improved DET with GOd and improved electrode temporal stability, using enzyme entrapment in a porous ionic liquid (1-ethyl-3-methylimidazolium acetate, EMIM-acetate) reconstituted cellulose layer that was 40 embedded with partially oxidised MWCNT 5 . MWCNT adsorbed onto the electrode surface increased electronic contact between the electrode and the enzyme whilst improving electrostatic interactions between the enzyme and MWCNT 9 . In method 1, an ionic liquid reconstituted cellulose/MWCNT layer was firstly applied to a glassy carbon (GC) electrode surface and followed by adsorption of GOd. The ionic liquid/cellulose/MWCNT matrix was also mixed with GOd and 60 applied to a GC electrode, giving the method 2 bioelectrode. For method 3, GOd was directly adsorbed onto a GC electrode and covered with an ionic liquid reconstituted cellulose/MWCNT layer, whereas in method 4, a GC electrode was covered with MWCNT prior to GOd adsorption and finally covered with an 65 ionic liquid reconstituted cellulose/MWCNT layer. The partially oxidised MWCNT were used to improve electronic connection between GOd and the GC electrode surface whilst attempting to improve bioelectrode sta...

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Section: Discussionmentioning

confidence: 99%

An optimised glucose oxidase bioelectrode exhibiting high performance direct electron transfer

Milton

Baur

Varcoe

et al. 2012

Phys. Chem. Chem. Phys.

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“…To the best of our efforts, various recently reported GOD glucose biosensors based on oxygen reduction are summarized in Table 1 with respect to the sensing material, sensing technique, the limit of detection (LOD), sensitivity, the linear range, K m,app , and the electron transfer rate constant (k s ). It can be seen that the performance of the developed glucose biosensor is among the best [33,34,36,[58][59][60][61][62][63][64][65].…”

Section: Electrochemical Reduction Of O 2 At the Modified Electrodes mentioning

confidence: 99%

Glucose Biosensor Using Glucose Oxidase and Electrospun Mn₂O₃‐Ag Nanofibers

et al. 2011

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The highly porous Mn 2 O 3 -Ag nanofibers were fabricated by a facile two-step procedure (electrospinning and calcination). The structure and composition of the Mn 2 O 3 -Ag nanofibers were characterized by SEM, TEM, XRD, EDX and SAED. The as-prepared Mn 2 O 3 -Ag nanofibers were then employed as the immobilization matrix for glucose oxidase (GOD) to construct an amperometric glucose biosensor. The biosensor shows fast response to glucose, high sensitivity (40.60 mA mM À1 cm À2 ), low detection limit (1.73 mM at S/N = 3), low K m,app value and excellent selectivity. These results indicate that the novel Mn 2 O 3 -Ag nanfibers-GOD composite has great potential application in oxygen-reduction based glucose biosensing.

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“…Several type of CNT electrodes have been reported, including CNT paste [9], CNT film coated electrode [10], CNT powder microelectrode [11] and CNT paper electrode [12]. The results reported by various researchers suggest that CNT can be used to improve electrons transfer reactions of many desired molecules such as dopamine [13], b-nicotinamide adenine dinucleotide (NADH) [14], epinephrine [15], glucose [16], ascorbic acid [17], and uric acid [18].…”

Section: Introductionmentioning

confidence: 96%

Electrocatalytic determination of sumatriptan on the surface of carbon-paste electrode modified with a composite of cobalt/Schiff-base complex and carbon nanotube

Amiri

Pakdel

Bezaatpour

et al. 2011

Bioelectrochemistry

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Amperometric glucose sensor based on glucose oxidase immobilized on gelatin-multiwalled carbon nanotube modified glassy carbon electrode

Cited by 191 publications

References 35 publications

An optimised glucose oxidase bioelectrode exhibiting high performance direct electron transfer

An optimised glucose oxidase bioelectrode exhibiting high performance direct electron transfer

Glucose Biosensor Using Glucose Oxidase and Electrospun Mn₂O₃‐Ag Nanofibers

Electrocatalytic determination of sumatriptan on the surface of carbon-paste electrode modified with a composite of cobalt/Schiff-base complex and carbon nanotube

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Amperometric glucose sensor based on glucose oxidase immobilized on gelatin-multiwalled carbon nanotube modified glassy carbon electrode

Cited by 191 publications

References 35 publications

An optimised glucose oxidase bioelectrode exhibiting high performance direct electron transfer

An optimised glucose oxidase bioelectrode exhibiting high performance direct electron transfer

Glucose Biosensor Using Glucose Oxidase and Electrospun Mn2O3‐Ag Nanofibers

Electrocatalytic determination of sumatriptan on the surface of carbon-paste electrode modified with a composite of cobalt/Schiff-base complex and carbon nanotube

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Product

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Glucose Biosensor Using Glucose Oxidase and Electrospun Mn₂O₃‐Ag Nanofibers