Amperometric Immunosensor for Chagas’ Disease Using Gold CD‐R Transducer

Abstract

Chagas disease has been spread mainly through blood transfusion or blood components, therefore the development of a device for neglected diseases with high specificity and sensitivity is important to public health. This work describes the development of an amperometric immunosensor for determination of Chagas disease through a goldbased electrode obtained from a recordable compact disc modified with 4-(methylmercapto)benzaldehyde for the immobilization of Tc85 protein of the Trypanosoma cruzi. The immunoassays were carried out using positive and negative sera from Chagasdisease patients and immunoglobulin conjugated with peroxidase enzyme. The immunosensor presented À0.949 mA as cut-off value and was applied in sera samples.

Biosensors to Diagnose Chagas Disease: A Brief Review

“…Biosensors that have been investigated for the diagnosis of CD can be classified into electrochemical and optical. Among electrochemical sensors, amperometric [ 33 , 34 , 35 , 36 ] and impedimetric [ 37 ] sensors can be found, whereas in optical sensors only surface plasmon resonance (SPR) transducers [ 6 ] are reported. Biosensors could provide the benefits presented in Table 1 compared to other currently employed techniques for the diagnosis of CD.…”

The role of nanogold in human tropical diseases: research, detection and therapy

“…Foguel et al [29] used the same principle for the construction of an amperometric imunosensor. The novelty here was the gold based electrode being obtained from a recordable compact disc (CD-R), then modified with 4-(methylmercapto)benzaldehyde for the immobilization of Tc85 protein of the T. cruzi.…”

“…As Foguel et al [29] suggested for T. Cruzi detection, Cavalcanti et al [82] also used a gold film electrode obtained from a recordable compact disk, but for the detection of non-structural protein 1 (NS1) of the dengue virus. This protein is abundantly present in blood during the acute phase of the dengue infection, in a correlation with viremia levels and, consequently, can be used to early diagnostic of the dengue hemorrhagic fever.…”

Novel Electrochemical Biosensing for Detection of Neglected Tropical Parasites of Animal Origin: Recent Advances

“…The biosensor were shown a good reproducibility and efficiency, but it shown a cross reactivity with Leishmania [29]. An immunosensors was constructed for sero diagnosis chagas disease by Vinicius Foguel et al [30]. The gold electrode was fabricated by acid etched recordable compact disc (CDÀ R) and moderated with monolayer of 4-(meth-ylmecapto) benzaldehyde (SBZA) to immobilize the T. cruzi antigen (Tc85).…”