2016
DOI: 10.1038/srep30905
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Amperometric inhibitive biosensor based on horseradish peroxidase-nanoporous gold for sulfide determination

Abstract: As a well-known toxic pollutant, sulfide is harmful to human health. In this study, a simple and sensitive amperometric inhibitive biosensor was developed for the determination of sulfide in the environment. By immobilizing nanoporous gold (NPG) on glassy carbon electrode (GCE), and encapsulating horseradish peroxidase (HRP) onto NPG, a HRP/NPG/GCE bioelectrode for sulfide detection was successfully constructed based on the inhibition of sulfide on HRP activity with o-Phenylenediamine (OPD) as a substrate. The… Show more

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Cited by 14 publications
(7 citation statements)
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“…Many of these techniques have intrinsic limitations, such as being time-consuming, requiring pre-treatment of the sample, being labour intensive, and requiring skilled handling. In contrast, electrochemical methods are considered as one of the most promising approaches because of low cost, simplicity, high sensitivity and specificity, which is ideal for point-of care, implantable and portable devices [ 18 , 19 , 20 ]. Amongst neurochemical devices, a range of amperometric biosensors have been developed based on loaded glutamate oxidase enzyme [ 21 , 22 , 23 ].…”
Section: Introductionmentioning
confidence: 99%
“…Many of these techniques have intrinsic limitations, such as being time-consuming, requiring pre-treatment of the sample, being labour intensive, and requiring skilled handling. In contrast, electrochemical methods are considered as one of the most promising approaches because of low cost, simplicity, high sensitivity and specificity, which is ideal for point-of care, implantable and portable devices [ 18 , 19 , 20 ]. Amongst neurochemical devices, a range of amperometric biosensors have been developed based on loaded glutamate oxidase enzyme [ 21 , 22 , 23 ].…”
Section: Introductionmentioning
confidence: 99%
“…In this configuration, the detection limit for glyphosate was 0.16 µg L −1 , much lower than the previous example and the metabolite of glyphosate, aminomethylphosphonic acid was detected at 1 µg L −1 [ 87 ]. Besides glyphosate, peroxidase is also inhibited by heavy metals [ 107 ], sulfides [ 108 ] etc. therefore a more holistic approach looking at all possible inhibitors within a real sample is desirable when developing a novel peroxidase-based biosensor.…”
Section: Other Enzymesmentioning
confidence: 99%
“…The development of sensitive and reliable diagnostic devices for various disease biomarkers is important for the early detection, management, and treatment of diseases [ 147 ]. Many disease biomarkers (such as antigens) are found as proteins and are specifically recognized from the biological fluids by a suitable antibody.…”
Section: Electrochemical Biosensingmentioning
confidence: 99%