Amperometric PDMS/glass capillary electrophoresis-based biosensor microchip for catechol and dopamine detection

Schöning, Michael J.; Jacobs, Michael; Muck, Alexander; Knobbe, D.-T.; Wang, J.; Chatrathi, Madhu Prakash; Spillmann, S.

doi:10.1016/j.snb.2004.11.032

Cited by 120 publications

(49 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Patterning of micron-sized gold dots on a glass surface It has been shown previously that vapor microbubbles can be generated via laser-induced localized heating of metal surfaces immersed in water (Yang, 2007) or nanoparticle dispersed tissue mimicking materials (Farny et al, 2005). To produce tandem bubbles with precision in nucleation sites and desirable spatial distribution, a glass surface was treated by coating it with an array of paired gold dots having a separation distance of 40 lm using metal lift-off technique (Schoning et al, 2005). Briefly, as shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

Dynamics of tandem bubble interaction in a microfluidic channel

Yuan

Sankin

Zhong

2011

The Journal of the Acoustical Society of America

View full text Add to dashboard Cite

The dynamics of tandem bubble interaction in a microfluidic channel (800 Â 21 lm, W Â H) have been investigated using high-speed photography, with resultant fluid motion characterized by particle imaging velocimetry. A single or tandem bubble is produced reliably via laser absorption by micron-sized gold dots (6 lm in diameter with 40 lm in separation distance) coated on a glass surface of the microfluidic channel. Using two pulsed Nd:YAG lasers at k ¼ 1064 nm and $10 lJ/ pulse, the dynamics of tandem bubble interaction (individual maximum bubble diameter of 50 lm with a corresponding collapse time of 5.7 ls) are examined at different phase delays. In close proximity (i.e., interbubble distance ¼ 40 lm or c ¼ 0.8), the tandem bubbles interact strongly with each other, leading to asymmetric deformation of the bubble walls and jet formation, as well as the production of two pairs of vortices in the surrounding fluid rotating in opposite directions. The direction and speed of the jet (up to 95 m/s), as well as the orientation and strength of the vortices can be varied by adjusting the phase delay.

show abstract

Section: Methodsmentioning

confidence: 99%

Dynamics of tandem bubble interaction in a microfluidic channel

Yuan

Sankin

Zhong

2011

The Journal of the Acoustical Society of America

View full text Add to dashboard Cite

show abstract

“…To produce tandem bubbles with precision in nucleation sites and desirable spatial distribution, a glass surface was treated by coating it with an array of paired gold dots having a separation distance of 40 µm using metal lift-off technique [116,117]. Briefly, as shown in Fig.…”

Section: Patterning Of Micron-sized Gold Dots In Microfluidic Channelsmentioning

confidence: 99%

Probing the bioeffects of cavitation at the single-cell level

Yuan

Sankin

Yang

et al. 2013

The Journal of the Acoustical Society of America

View full text Add to dashboard Cite

The primary goal of this dissertation research is to develop an experimental system and associated techniques that can be used to investigate the bioeffects produced by cavitation bubbles at the single cell level. Such information has been lacking due to the randomness and complexity in cavitation inception and subsequent bubble-bubble interaction generated by an acoustic field typically used in therapeutic ultrasound applications.Connection between cavitation activities and bioeffects produced in cells nearby presents another challenge that has not been resolved satisfactorily. In this work, we developed a laser-based system for generating tandem bubbles with a maximum diameter about 50 µm (i.e., on the scale of a single cell) in a microfluidic channel of 25 µm in height and 800 µm in width. We further developed techniques for micropatterning of individual gold dots (15 nm thick and 6 µm in diameter) used for bubble generation, which are precisely aligned at various stand-off distances (SD) from individual islands (32 x32 µm 2 ) coated with fibronectin used for cell adhesion. The dynamics of tandem bubble interaction with resultant jet formation, microstreaming and vortex flow in the microfluidic channel were captured by high-speed imaging and particle image velocimetry (PIV). The deformation of the target cell was recorded by high-speed imaging as well (using a second camera) immediately after the tandem bubble interaction and assessment of membrane strain was aided with 2 µm sized polystyrene beads attached to the cell membrane.

show abstract

“…For example, the biosensors based on the field effect transistor (FET) made by MEMS immobilize anti-PSA on the carbon nanotubes (CNTs) (Kojima et al, 2005), liquid-chromatography-based biochip detects peptide mixture (Xie et al, 2005), and the biochip combines PCR-based DNA amplification and electrochemical detection (Lee et al, 2003) have been reported. Other few examples include antibody-based chips for determining protein isoform (Loonberg & Carlsson, 2006), liquid-chromatography-based chips for detecting peptide mixture (Xie et al, 2005), and electrophoresis-based chips for sensing catechol and dopamine (Schoning et al, 2005). Moreover, there are many choices for the materials of the microchannel, such as poly(dimethylsiloxane) (PDMS) (McDonald et al, 2000), poly(methyl-methacrylate) (PMMA) (Ford et al, 1998) and polycarbonate (PC) (Liu et al, 2001) … etc.…”

Section: Reviews and Motivationsmentioning

confidence: 99%