Amperometric sensor for L-ascorbic acid determination based on MnO 2 bulk modified screen printed electrode

Abstract

A simple biosensor constructed by bulk-modification of carbon ink with manganese dioxide as a mediator was investigated for its ability to serve as amperometric detector for L-ascorbic acid in hydrodynamic mode. The sensor could be operated at pH 5.0 (0.05 M phosphate buffer) and exhibited excellent reproducibility and stability. Optimization of measurement parameters such as applied working potential and pH value were studied in detail. The screen printed electrode exhibited a linear amperometric increase with the concentration of L-ascorbic acid from 50 mg L(-1) to 250 mg L(-1) and gave a (LOD = 3sigma) detection limit of 0.2 mg L(-1) (1.172 micromol L(-1)). The manganese dioxide modified screen printed electrode shows long term stability.

Manganese Dioxide Graphite Composite Electrodes: Application to the Electroanalysis of Hydrogen Peroxide, Ascorbic Acid and Nitrite

“…The calibration curve exhibited a linear range up to 2.5 mM with a limit of detection (based on 3σ) for ascorbic acid of 0.4 μM. This detection limit is lower or comparable to the ones obtained with existing methodologies [35][36][37][38] that range from 0.08 to 71 μM as shown in Table 2.…”

Determination of H2O2 by MnO2 modified screen printed carbon electrode during Fenton and visible light-assisted photo-Fenton based removal of acetamiprid from water

“…These electrodes are of low cost, they are environmentally friendly and usually require only small volumes of the investigated samples. SPCE bulk-or surface-modified with MnO 2 can be successfully applied for the determination of H 2 O 2 [26,29], L-ascorbic acid [45], choline [46], glutamate [47], glucose [26][27][28][29]48], as well as biogenic amines [49].…”

A new, improved sensor for ascorbate determination at copper hexacyanoferrate modified carbon film electrodes

“…The response to ascorbate at 0.00 and +0.025 V was poor, but at +0.05 V the response was sufficiently good to determine ascorbate in the micromolar range. At higher potentials the sensitivity increased further, but was never better than other existing sensors [14,[17][18][19][20][21]. Therefore, the operating potential was chosen to be +0.05 V. All three types of CuHCF films (deposited by cycling of the potential, galvanostatically and chemically) were calibrated for the ascorbate anion.…”

“…Other amperometric AA sensors include electrodes chemically modified with 7,7,8,8-tetracyanoquinodimethane [16], osmium 2,2¢-bipyridyl poly-4-vinylpyridine chloride complex [17], lanthanum 2,6-dichlorophenolindophenol [18], electropolymerized N,N-dimethylaniline [19], manganese dioxide [20] and a cobalt (II) phthalocyanine doped with iodine for potentiometric determination [21]; a hanging mercury drop electrode [22] was also used to oxidise AA. The electrocatalytic oxidation of AA on conducting polymer electrodes, in particular on polyaniline, has also been studied [23].…”