Amphiphilicity in Homopolymer Surfaces Reduces Nonspecific Protein Adsorption

Chen, Yangbin; Thayumanavan, S.

doi:10.1021/la901692a

Cited by 38 publications

(28 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the proteins with plentiful charges from different amino acids could be easily to adhere to the PSANMs surface due to electrostatic interactions . As illustrated in Figure , after the PSANMs were cultured in plasma, their negative charge remarkably decreased.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the proteins with plentiful charges from different amino acids could be easily to adhere to the PSANMs surface due to electrostatic interactions. [41,42] As illustrated in Figure 4, after the PSANMs were cultured in plasma, their negative charge remarkably decreased. All bare PSANMs showed > 40 % decrease in negative charge (Table S4), suggesting that negatively charged PSANMs caused severe non-specific protein adsorption.…”

Section: Anti-biofouling Effect Of Pegylated Psanmsmentioning

confidence: 99%

See 1 more Smart Citation

Hydrophilic Porous Poly(l‐Lactic Acid) Nanomembranes: Self‐Assembly, and Anti‐Biofouling and Anti‐Thrombosis Effects

et al. 2019

View full text Add to dashboard Cite

Hydrophilic PEGylated porous self‐assembled nanomembranes (PSANMs) with average thickness and pore diameter of ca. 10 and 20–24 nm were successfully prepared by an emulsification‐induced programmable self‐assembly strategy. The hydrophilicity, anti‐biofouling, and anti‐thrombosis properties of PEGylated PSANMs were largely improved in comparison with the nonfunctionalized PSANMs, which could transform into hydrophilic (PEGylated PSANMs, minimum water contact angle: 38.8°) from hydrophobic units (PSANMs, maximum water contact angle: 137.5°) with increasing PEG density and length. The total protein adsorption of PEGylated PSANMs was about six times lower than that of the PSANMs, while the thrombosis of the PEGylated PSANMs (maximum R‐time: 5.37 min) was also greatly relieved in comparison with the PSANMs (minimum R‐time: 2.93 min). Such PEG‐modified PSANMs may have applications in drug delivery and tissue and organ repair in vivo.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Anti-biofouling Effect Of Pegylated Psanmsmentioning

confidence: 99%

Hydrophilic Porous Poly(l‐Lactic Acid) Nanomembranes: Self‐Assembly, and Anti‐Biofouling and Anti‐Thrombosis Effects

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Chen et al [29] studied the interactions of amphiphilic polymers films with proteins and found that despite the high affinity of the hydrophilic or hydrophobic functional groups by themselves toward proteins, the films showed great reduced nonspecific protein adsorption. Colak et al [26] designed a series of novel zwitterion-based, amphiphilic coatings.…”

Section: Introductionmentioning

confidence: 99%

Hemocompatibility Evaluation of Polyurethane Film with Surface-Grafted Sugar- Based Amphipathic Compounds

Xin¹,

Du²,

Wang³

et al. 2017

J Anal Bioanal Tech

View full text Add to dashboard Cite

Sugar-based amphipathic compounds (BA-CnAG) were successfully prepared. Polyurethane (PU) was grafted with glycidyl methacrylate (GMA) by the means of UV irradiation, and further modified with the BA-CnAG based on the ring opening of the epoxy groups. The surface graft polymerization was confirmed by ATR-FTIR and XPS. Water contact angle, protein adsorption, and platelet adhesion measurements were used to evaluate the hydrophilicity and hemocompatibility of the films. The results demonstrated that amphiphilic surfactant-containing polymer surfaces presented protein-resistant behavior and anti-platelet adhesion after functionalization with BA-CnAG. Besides, the hemocompatibility of the modified surface deteriorated as the length of hydrophobic chain of BA-CnAG increased. glycidyl methacrylate (GMA) were obtained from Shanghai Aladdin Chemicals (China). Benzophenone (BP) was supplied by Peking Ruichen Chemicals (China). Bovine serum albumin (BSA), sodium dodecyl sulfate (SDS) and phosphate buffered solution (PBS, 0.01 mol/L, pH=7.4) were provided by Dingguo Bio-technology (China). Micro BCATM protein assay reagent kits were purchased from Boster Biological Technology (AR1110, China). The platelet-rich plasma (PRP) was obtained from the fresh rabbit blood by centrifugation at 1000 rpm for 15 min. The other solvents and reagents were AR grade chemicals and used without further purification. Preparation and characterization of sugar-based amphipathic compoundsSynthesis of Boc-Asp: To a solution of Asp (10 mmol) in acetone/ ultrapure water (10:1, 44 mL), was added triethylamine (20 mmol), and the resulting solution was stirred in an ice-water bath. (B OC ) 2 O (11 mmol) was added dropwise, stirred for 3 h. The solution was concentrated under reduced pressure, diluted with H 2 O (20 mL), Scheme 2: Schematics of the PU surface modified with sugar-based amphipathic compounds.

show abstract

“…[35,36] Indeed, polymer surfaces modified by amphiphilic materials with delicate balance in every repeat unite can present magnificent protein adsorption resistance. [37] Therefore, we hypothesized that amphiphilic surfactant-containing polymer surfaces will obtain a low protein adsorption as well as a promising hemocompatibility. To test this hypothesis, two types of sugar-based trisiloxane surfactants were synthesized as potential surface modifiers.…”

Section: Introductionmentioning

confidence: 99%

Surface modification of polyurethane via covalent immobilization of sugar-based trisiloxane surfactants

Xin

Yan

et al. 2015

Designed Monomers and Polymers

View full text Add to dashboard Cite

Sugar-based trisiloxane surfactants (N-siloxanegluconamides and N-siloxanelactobionamides, denoted as Si(3)NGA and Si(3)NLA) with the amphiphilic structures were successfully prepared. Polyurethane (PU) was grafted with 2-carboxyethyl acrylate (CEA) by the means of UV-induced graft polymerization, and further introduction of the Si(3)NGA and Si (3)NLA by conjugating the amino groups and acid groups. The surface graft polymerization was confirmed by attenuated total reflection Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, respectively. Water contact angle, protein adsorption, and platelet adhesion measurements were used to evaluate the hydrophilicity and hemocompatibility of the films. It was found that the Si(3)NGA-modified substrates obviously suppressed protein adsorption and platelet adhesion, while Si(3)NLA-modified substrates exhibited a higher protein adsorption and a coagulating performance of blood.

show abstract

Amphiphilicity in Homopolymer Surfaces Reduces Nonspecific Protein Adsorption

Cited by 38 publications

References 35 publications

Hydrophilic Porous Poly(l‐Lactic Acid) Nanomembranes: Self‐Assembly, and Anti‐Biofouling and Anti‐Thrombosis Effects

Hydrophilic Porous Poly(l‐Lactic Acid) Nanomembranes: Self‐Assembly, and Anti‐Biofouling and Anti‐Thrombosis Effects

Hemocompatibility Evaluation of Polyurethane Film with Surface-Grafted Sugar- Based Amphipathic Compounds

Surface modification of polyurethane via covalent immobilization of sugar-based trisiloxane surfactants

Contact Info

Product

Resources

About