Amphiregulin is much more abundantly expressed than transforming growth factor-alpha and epidermal growth factor in human follicular fluid obtained from patients undergoing in vitro fertilization–embryo transfer

Inoue, Yuichi; Miyamoto, Shingo; Fukami, T.; Shirota, Kyoko; Yotsumoto, Fusanori; Kawarabayashi, Tatsuhiko

doi:10.1016/j.fertnstert.2008.01.014

Cited by 44 publications

(38 citation statements)

References 35 publications

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“…At the same time, 14% more MII oocytes and 11% more transferable embryos were seen in the GnRH-a group. These findings confirm a recent trial by Inoue et al (8), measuring three different EGF receptor ligands: EGF, transforming growth factor a, and AR, in FF obtained from patients undergoing IVF treatment. The authors found that AR was the most abundant EGF receptor ligand expressed in FF and that the concentration of AR in FF was 3,000 times as high as that in serum.…”

Section: Discussionsupporting

confidence: 91%

“…Moreover, a trend for an inverse correlation was found for AR and embryo quality. Finally, a significant inverse correlation between AR and FF exogenous hCG was found in the group of patients who conceived (8), which could indicate a down-regulation of AR synthesis because of the prolonged action of hCG.…”

Section: Discussionmentioning

confidence: 86%

“…Recently, isolated murine cumulus enclosed oocytes have been shown dose-dependently to respond to AR by resuming meiosis (7). In humans, AR becomes expressed abundantly in preovulatory follicles on exposure to the midcycle surge of gonadotrophins or hCG, and the concentration of AR has been shown to correlate inversely with the fertilization rate (8).…”

mentioning

confidence: 99%

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Levels of the epidermal growth factor-like peptide amphiregulin in follicular fluid reflect the mode of triggering ovulation: a comparison between gonadotrophin-releasing hormone agonist and urinary human chorionic gonadotrophin

Humaıdan

Westergaard

Mikkelsen

et al. 2011

Fertility and Sterility

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 86%

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Levels of the epidermal growth factor-like peptide amphiregulin in follicular fluid reflect the mode of triggering ovulation: a comparison between gonadotrophin-releasing hormone agonist and urinary human chorionic gonadotrophin

Humaıdan

Westergaard

Mikkelsen

et al. 2011

Fertility and Sterility

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“…In addition, our previous study also demonstrated that AREG level in follicular fluid was positively correlated with the number of available embryos (Liu et al, 2012). Some contrary reports, however, have shown that the level of AREG in follicular fluid was inversely correlated with the fertilization rate (Inoue et al, 2009), and AREG mRNA expression was lower in cumulus granulosa cells enclosing oocytes achieving blastocyst development at days 5 or 6 than those who failed to achieve blastocyst development (Feuerstein et al, 2007). In Feuerstein's research (2007), however, AREG expression in cumulus granulosa cells was significantly higher after nuclear maturation, whereas no The data were shown as fold-change compared with the control (0 h) (mean ± SD); (B) comparison of fold-changes of AREG and EREG expression at 4 h after the HCG stimulation between the pregnant and non-pregnant groups (mean ± SD) (*P < 0.05 versus the non-pregnant group); (C) the increase of AREG expression at 4 h after stimulation was positively correlated with the number of good quality embryos (P < 0.05).…”

Section: Discussionmentioning

confidence: 60%

Altered amphiregulin expression induced by diverse luteinizing hormone receptor reactivity in granulosa cells affects IVF outcomes

Huang

Zhao

et al. 2015

Reproductive BioMedicine Online

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The expression of specific genes (LHR, AREG, EREG, EGFR, NPPC and NPR2) involved in peri-ovulatory signalling pathways induced by LH surge in granulosa cells was investigated, and their relationships with IVF outcomes analysed. mRNA levels of the genes of 147 infertile women undergoing IVF and intracytoplasmic sperm injection (ICSI) with embryo transfer were evaluated. Compared with non-pregnant women, amphiregulin (AREG) mRNA levels in mural and cumulus graunulosa cells were significantly higher (P < 0.05) in pregnant women, and were positively correlated with number of oocytes retrieved and good-quality embryos. No significant differences were found between the two groups in the remaining detected genes. To investigate the reason for the differences in AREG expression, mural granulosa cells were cultured and stimulated with human chorionic gonadotrophin (HCG) for 2-24 h. At 4 h after HCG stimulation, AREG and epiregulin mRNA expression peaked, with much greater increases in the pregnant group. The fold-change of AREG expression was positively correlated with number of good-quality embryos. No obvious correlation, however, was found between NPPC/Npr2 expression levels in granulosa cells and IVF outcomes. Altered AREG expression induced by diverse luteinizing hormone receptor reactivity in granulosa cells may provide a useful marker for oocyte developmental competency.

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“…Although Egfr Ϫ/Ϫ mice are embryonic lethal, mice with a hypomorphic Egfr allele (Egfr wa2 ) are viable, and Areg Ϫ/Ϫ Egfr wa2/wa2 double mutant females showed more profound defects in induction of Ptgs2, Has2, and Tnfaip6, cumulus expansion, and ovulation after gonadotropin administration (345). In support of an evolutionarily conserved role for EGF-like growth factors in cumulus expansion, amphiregulin is abundant in human follicular fluid obtained from patients undergoing in vitro fertilization (IVF) (346,347).…”

Section: F the Preovulatory Follicle Cumulus Expansion Ovulation mentioning

confidence: 99%

The Mammalian Ovary from Genesis to Revelation

2009

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Two major functions of the mammalian ovary are the production of germ cells (oocytes), which allow continuation of the species, and the generation of bioactive molecules, primarily steroids (mainly estrogens and progestins) and peptide growth factors, which are critical for ovarian function, regulation of the hypothalamic-pituitary-ovarian axis, and development of secondary sex characteristics. The female germline is created during embryogenesis when the precursors of primordial germ cells differentiate from somatic lineages of the embryo and take a unique route to reach the urogenital ridge. This undifferentiated gonad will differentiate along a female pathway, and the newly formed oocytes will proliferate and subsequently enter meiosis. At this point, the oocyte has two alternative fates: die, a common destiny of millions of oocytes, or be fertilized, a fate of at most approximately 100 oocytes, depending on the species. At every step from germline development and ovary formation to oogenesis and ovarian development and differentiation, there are coordinated interactions of hundreds of proteins and small RNAs. These studies have helped reproductive biologists to understand not only the normal functioning of the ovary but also the pathophysiology and genetics of diseases such as infertility and ovarian cancer. Over the last two decades, parallel progress has been made in the assisted reproductive technology clinic including better hormonal preparations, prenatal genetic testing, and optimal oocyte and embryo analysis and cryopreservation. Clearly, we have learned much about the mammalian ovary and manipulating its most important cargo, the oocyte, since the birth of Louise Brown over 30 yr ago.

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Amphiregulin is much more abundantly expressed than transforming growth factor-alpha and epidermal growth factor in human follicular fluid obtained from patients undergoing in vitro fertilization–embryo transfer

Cited by 44 publications

References 35 publications

Levels of the epidermal growth factor-like peptide amphiregulin in follicular fluid reflect the mode of triggering ovulation: a comparison between gonadotrophin-releasing hormone agonist and urinary human chorionic gonadotrophin

Levels of the epidermal growth factor-like peptide amphiregulin in follicular fluid reflect the mode of triggering ovulation: a comparison between gonadotrophin-releasing hormone agonist and urinary human chorionic gonadotrophin

Altered amphiregulin expression induced by diverse luteinizing hormone receptor reactivity in granulosa cells affects IVF outcomes

The Mammalian Ovary from Genesis to Revelation

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