Amphiregulin regulates proliferation and migration of HER2-positive breast cancer cells

Schmucker, Hannah; Blanding, Walker M.; Mook, Julia; Wade, Jessica F; Park, Jang Pyo; Kwist, Kerri W.; Shah, Hiral; Booth, Brian W.

doi:10.1007/s13402-017-0363-3

Cited by 20 publications

(11 citation statements)

References 46 publications

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“…Skin diseases, such as psoriasis, can be triggered by physical injury in susceptible patients (known as the Koebner phenomenon), and it is critical to understand the elements participating in processes occurring in cutaneous wounds (44,45). Additionally, it has been suggested that endogenous AREG modulates tissue repair and regulates the migration of several cell types, such as breast cancer cells (46,47). The current findings confirmed that NMD perturbation in keratinocytes promoted wound re-epithelialization by adjusting endogenous AREG levels.…”

Section: Discussionsupporting

confidence: 77%

Aberrant expression of the UPF1 RNA surveillance gene disturbs keratinocyte homeostasis by stabilizing AREG

Cheng

Shi

et al. 2020

Int J Mol Med

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The up-frameshift suppressor 1 homolog (UPF1) RNA surveillance gene is a core element in the nonsense-mediated RNA decay (NMd) pathway, which impacts a broad spectrum of biological processes in a cell-specific manner. In the present study, the contribution of the NMd pathway to psoriasis lesions and its moderating effects on the biological processes of keratinocytes was reported. Sanger sequencing for skin scales from two patients with psoriasis identified two mRNA mutations (c.2935_2936insA and c.2030-2081del) in the UPF1 gene. The somatic mutants produced truncated UPF1 proteins and perturbed the NMd pathway in cells, leading to the upregulation of NMD substrates. As the most abundant epidermal growth factor receptor ligand in keratinocytes, it was concluded that amphiregulin (AREG) mRNA is a natural NMd substrate, that is dependent on its 3' untranslated region sequence. Perturbed NMD modulated keratinocyte homeostasis in an AREG-dependent but nonidentical manner, which highlighted the unique characteristics of NMD in keratinocytes. By targeting AREG mRNA post-transcriptionally, the UPF1-NMd pathway contributed to an imbalance between proliferation on the one hand, and apoptosis and abnormal differentiation, migration and inflammatory response on the other, in keratinocytes, which indicated a role of the NMd pathway in the full development of keratinocyte-related morbidity and skin diseases.

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Section: Discussionsupporting

confidence: 77%

Aberrant expression of the UPF1 RNA surveillance gene disturbs keratinocyte homeostasis by stabilizing AREG

Cheng

Shi

et al. 2020

Int J Mol Med

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“…Cell invasion and migration are important processes in breast cancer development and metastasis and have become a challenging problem in clinical treatment 40. The Transwell assays suggested that treatment with higher doses of nanocomposites can significantly reduce the number of invaded cells, and the effects showed a dose dependent (Figure 7B).…”

Section: Resultsmentioning

confidence: 99%

<p>Magnetic And pH Dual-Responsive Nanoparticles For Synergistic Drug-Resistant Breast Cancer Chemo/Photodynamic Therapy</p>

Wang¹,

Li²,

Li³

et al. 2019

IJN

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BackgroundDrug resistance is one of the prime reasons of chemotherapy failure in breast cancer and is also an important factor affecting prognosis.PurposeIn this study, we constructed a functional magnetic mesoporous silica-based nanocomposite (MMSN) for breast cancer chemotherapy/photodynamic therapy.MethodsMMSN was characterized by scanning electron microscopy and transmission electron microscopy to observe the morphology. The size distribution and zeta potential of the MSNs were determined using Malvern Particle Size Analyzer. Anti-tumor activity in vitro was investigated by CCK-8 assay, flow cytometry and transwell experiment, and the anti-tumor activity in vivo was probed into by magnetic targeting, toxicity, and antitumor effects in breast cancer-bearing BABL/c nude mice.ResultsThe results showed that the release of doxorubicin in the nanocomposites was pH sensitive, and the cumulative release rate reached 80.53% at 60 h under acidic conditions. The nanocomposites had a high cellular uptake ability in MCF-7/ADR cells, and the IC50 value of the nanocomposites on MCF-7/ADR cells was 4.23 μg/mL, much smaller than that of free DOX (363.2 μg/mL). The nanocomposites could effectively reverse resistance and induce apoptosis of MCF-7/ADR cells. The blood biochemistry parameters and H&E staining results showed no serious adverse effects after treatment with the nanocomposites. Prussian blue staining showed that the nanocomposites were able to target tumor tissues in tumor-bearing mice under a magnetic field. The combined chemical/photodynamic therapy significantly inhibited tumor growth in vivo.ConclusionNanocomposites with magnetic and pH dual-responsive performance has shown a promising platform for enhanced drug-resistant breast cancer treatment.

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“…This data suggests that ILC2s could be activated by IL-33 to secrete IL-5 and IL-13 in the 4T1 model of breast cancer, but further investigation is required to confirm this finding also in patients. Moreover, it has been shown that AREG regulates the proliferation and the migration of different mouse and human estrogen-receptor positive (ER2 + ) breast cancer cell lines (87). However, it is still unknown whether ILC2s and ILC2-derived AREG are involved in this pro-tumoral axis.…”

Section: Ilc2s In Breast Cancermentioning

confidence: 99%

ILC2s: New Actors in Tumor Immunity

et al. 2019

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Innate lymphoid cells (ILCs) represent the most recently identified family of innate lymphocytes that act as first responders, maintaining tissue homeostasis and protecting epithelial barriers. In the last few years, group 2 ILCs (ILC2s) have emerged as key regulators in several immunological processes such as asthma and allergy. Whilst ILC2s are currently being evaluated as novel targets for immunotherapy in these diseases, their involvement in tumor immunity has only recently begun to be deciphered. Here, we provide a comprehensive overview of the pleiotropic roles of ILC2s in different tumor settings. Furthermore, we discuss how different therapeutic approaches targeting ILC2s could improve the efficacy of current tumor immunotherapies.

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Amphiregulin regulates proliferation and migration of HER2-positive breast cancer cells

Abstract: Based on our results we conclude that AREG is involved in regulating the proliferation and migration of erbB2/HER2-positive breast cancer cells.

Cited by 20 publications

References 46 publications

Aberrant expression of the UPF1 RNA surveillance gene disturbs keratinocyte homeostasis by stabilizing AREG

Aberrant expression of the UPF1 RNA surveillance gene disturbs keratinocyte homeostasis by stabilizing AREG

<p>Magnetic And pH Dual-Responsive Nanoparticles For Synergistic Drug-Resistant Breast Cancer Chemo/Photodynamic Therapy</p>

ILC2s: New Actors in Tumor Immunity

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