Amphoteric Behavior of Bovine Plasma Albumin and Its Detergent Complexes

Sulfhydryl-catalyzed isomerization of bovine mercaptalbumin

“…The solid line is a theoretical curve explained in the text. albumin, and Vijai and Foster (1967) and Decker and Foster (1967) used this approach on bovine plasma albumin.…”

“…A mobility vs. pH curve for A was constructed by drawing a smooth curve from the data of Vijai and Foster below pH 4 through the data of Nikkei at pH 4.8-5.3 and merging into the data of Decker at pH 7. To convert p to eip/kT, the factor of 7.29 X 103 V sec cm-2 used by Decker and Foster (1967) and Vijai and Foster (1967) was utilized for both N and A. Foster and Clark (1962) and Vijai and Foster (1967) made use of the method of Scatchard (Scatchard et al, 1957) to calculate pA)nt and n from a single plot of the data.…”

“…In the case of bovine plasma albumin, h ~hcoon below the isoionic pH; however, Vijai and Foster (1967) used infrared difference spectra to measure directly the number of protonated carboxylates at isoionic pH as 2.5. Decker and Foster (1967) concluded that there is 0.8 unprotonated histidine at isoionic pH. These same values of 2.5 protonated carboxyls and 0.8 unprotonated histidines were used to convert h to hcooh for native bovine mercaptalbumin.…”

“…The histidyl titration behavior of bovine mercaptalbumin is also somewhat complex. Decker and Foster (1967) suggested two classes of histidines, 10 with a normal pKint of 6.5 and 7 with a value of 7.5. Harmsen et al (1971) concluded the isoionic protein to have 8 histidines with a pKint of 6.9, 10 more histidines becoming unmasked and available for titration in the neutral transition.…”

Identification of competitive inhibitors for bovine serum albumin from dynamic combinatorial libraries containing a bienzyme system

“…For drug-protein interactions, 23 negative entropy is frequently taken as evidence for hydrogen bond and van der Waals' force, but it has been pointed out that hydrophobic interactions account for most of the binding energy when large organic anions such as fatty acids bind to BSA. 24,25 Studies with palmitate and stearate analogs containing spin-labeled nitroxyl groups also indicated that the binding energy was primarily derived from hydrophobic interactions extending along the entire length of the fatty acid chain. 26…”