Amphotericin B releasing nanoparticle topical treatment of Candida spp. in the setting of a burn wound

Sanchez, David A.; Schairer, David; Tuckman-Vernon, Chaim; Chouake, Jason; Kutner, Allison; Makdisi, Joy; Friedman, Joel M.; Nosanchuk, Joshua D.; Friedman, Adam

doi:10.1016/j.nano.2013.06.002

Cited by 79 publications

(46 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One can anticipate that NO, a gas to which microbes develop minimal resistance even following repeated exposures (60), may contribute to the efficacy of drugs to which microorganisms within biofilms have resistance. Additionally, this nanotechnology is a flexible platform to encapsulate antimicrobial drugs for local delivery into infected catheters in order to prevent biofilm formation or eradicate mature biofilms (61). Together, these findings underscore the clear translational potential for the utilization of NO-np in the prevention and treatment of biofilms infecting medical prosthetic devices.…”

Section: Discussionmentioning

confidence: 85%

Sustained Nitric Oxide-Releasing Nanoparticles Interfere with Methicillin-Resistant Staphylococcus aureus Adhesion and Biofilm Formation in a Rat Central Venous Catheter Model

Mihu

Cabral

Pattabhi

et al. 2017

Antimicrob Agents Chemother

Self Cite

View full text Add to dashboard Cite

Staphylococcus aureus is frequently isolated in the setting of infections of indwelling medical devices, which are mediated by the microbe's ability to form biofilms on a variety of surfaces. Biofilm-embedded bacteria are more resistant to antimicrobial agents than their planktonic counterparts and often cause chronic infections and sepsis, particularly in patients with prolonged hospitalizations. In this study, we demonstrate that sustained nitric oxide-releasing nanoparticles (NO-np) interfere with S. aureus adhesion and prevent biofilm formation on a rat central venous catheter (CVC) model of infection. Confocal and scanning electron microscopy showed that NO-np-treated staphylococcal biofilms displayed considerably reduced thicknesses and bacterial numbers compared to those of control biofilms in vitro and in vivo, respectively. Although both phenotypes, planktonic and biofilmassociated staphylococci, of multiple clinical strains were susceptible to NO-np, bacteria within biofilms were more resistant to killing than their planktonic counterparts. Furthermore, chitosan, a biopolymer found in the exoskeleton of crustaceans and structurally integrated into the nanoparticles, seems to add considerable antimicrobial activity to the technology. Our findings suggest promising development and translational potential of NO-np for use as a prophylactic or therapeutic against bacterial biofilms on CVCs and other medical devices.

show abstract

Section: Discussionmentioning

confidence: 85%

Sustained Nitric Oxide-Releasing Nanoparticles Interfere with Methicillin-Resistant Staphylococcus aureus Adhesion and Biofilm Formation in a Rat Central Venous Catheter Model

Mihu

Cabral

Pattabhi

et al. 2017

Antimicrob Agents Chemother

Self Cite

View full text Add to dashboard Cite

show abstract

“…We further evaluated the effects of FL2 NPsi on wound healing in a murine thermal burn wound model (Macherla et al 2012; Sanchez et al 2014). In contrast to excision wounds, full thickness thermal burn wounds are more traumatic secondary to heat-induced tissue coagulation, and healing is slower due to the edema, extensive necrosis, and hypoxia (Benson et al 2006).…”

Section: Resultsmentioning

confidence: 99%

Fidgetin-Like 2: A Microtubule-Based Regulator of Wound Healing

Charafeddine

Makdisi

Schairer

et al. 2015

Journal of Investigative Dermatology

Self Cite

View full text Add to dashboard Cite

Wound healing is a complex process driven largely by the migration of a variety of distinct cell types from the wound margin into the wound zone. In this study, we identify the previously uncharacterized microtubule-severing enzyme, Fidgetin-like 2 (FL2), as a fundamental regulator of cell migration that can be targeted in vivo using nanoparticle-encapsulated siRNA to promote wound closure and regeneration. In vitro, depletion of FL2 from mammalian tissue culture cells results in a more than two-fold increase in the rate of cell movement, due in part to a significant increase in directional motility. Immunofluorescence analyses indicate that FL2 normally localizes to the cell edge, importantly to the leading edge of polarized cells, where it regulates the organization and dynamics of the microtubule cytoskeleton. To clinically translate these findings, we utilized a nanoparticle-based siRNA delivery platform to locally deplete FL2 in both murine full-thickness excisional and burn wounds. Topical application of FL2 siRNA nanoparticles to either wound type results in a significant enhancement in the rate and quality of wound closure both clinically and histologically relative to controls. Taken together, these results identify FL2 as a promising therapeutic target to promote the regeneration and repair of cutaneous wounds.

show abstract

“…18 Sol-gel technology is also cost effective, biocompatible, and nontoxic. 17 Our platform has been employed to encapsulate agents ranging from nitric oxide for infection and wound healing 15,19 to amphotericin B for fungal burn wound infections 20 to sildenafil for erectile dysfunction. 21 The versatility of our nanoformulation allows for loading of different active ingredients, with therapeutic efficacy when applied topically, intradermally, and intravenously.…”

Section: Introductionmentioning

confidence: 99%

Curcumin-encapsulated nanoparticles as innovative antimicrobial and wound healing agent

Krausz

Adler

Cabral

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

Self Cite

420

273

View full text Add to dashboard Cite

Burn wounds are often complicated by bacterial infection, contributing to morbidity and mortality. Agents commonly used to treat burn wound infection are limited by toxicity, incomplete microbial coverage, inadequate penetration, and rising resistance. Curcumin is a naturally derived substance with innate antimicrobial and wound healing properties. Acting by multiple mechanisms, curcumin is less likely than current antibiotics to select for resistant bacteria. Curcumin's poor aqueous solubility and rapid degradation profile hinder usage; nanoparticle encapsulation overcomes this pitfall and enables extended topical delivery of curcumin. In this study, we synthesized and characterized curcumin nanoparticles (curc-np), which inhibited in vitro growth of methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa in dose-dependent fashion, and inhibited MRSA growth and enhanced wound healing in an in vivo murine wound model. Curc-np may represent a novel topical antimicrobial and wound healing adjuvant for infected burn wounds and other cutaneous injuries.

show abstract

Amphotericin B releasing nanoparticle topical treatment of Candida spp. in the setting of a burn wound

Cited by 79 publications

References 29 publications

Sustained Nitric Oxide-Releasing Nanoparticles Interfere with Methicillin-Resistant Staphylococcus aureus Adhesion and Biofilm Formation in a Rat Central Venous Catheter Model

Sustained Nitric Oxide-Releasing Nanoparticles Interfere with Methicillin-Resistant Staphylococcus aureus Adhesion and Biofilm Formation in a Rat Central Venous Catheter Model

Fidgetin-Like 2: A Microtubule-Based Regulator of Wound Healing

Curcumin-encapsulated nanoparticles as innovative antimicrobial and wound healing agent

Contact Info

Product

Resources

About