Amphritea opalescens sp. nov., isolated from sediment adjacent to Fildes Peninsula, Antarctica

Han, Xiaoyan; Zhang, Jing; Zhang, Yanfeng; Liu, Juanjuan; Fang, Wei; Fang, Zemin; Xiao, Yazhong

doi:10.1099/ijsem.0.003359

Cited by 8 publications

(20 citation statements)

References 19 publications

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“…The polar lipids of strains ZJ14W T and RP18W consisted of phosphatidylethanolamine and phosphatidylglycerol, that were similar to the polar lipid profiles of other members within the genus Amphritea (Fig. S5) [6–8].…”

Section: Full-textmentioning

confidence: 67%

“…in 2008 belongs to the family Oceanospirillaceae within the class Gammaproteobacteria and takes Amphritea atlantica M41 T as type species [4]. Since then, five novel species of this genus were reported, including Amphritea japonica JAMM 1866 T , Amphritea balenae JAMM 1525 T , Amphritea ceti RA1 T , Amphritea spongicola MEBiC05461 T and Amphritea opalescens ANRC-JH13 T [5–8]. The species of genera Bowmanella and Amphritea are all Gram-stain-negative, rod-shaped and mainly isolated from marine environments.…”

Section: Full-textmentioning

confidence: 99%

“…The type strain is Y57 T . The OrthoANIu ; 5, Amphritea ceti RA1 T (data from Kim et al [6]); 6, Amphritea balenae JCM 14781 T (data from Kim et al [6]); 7, Amphritea japonica JCM 14782 T (data from Kim et al [6]); 8, Amphritea opalescens ANRC-JH13 T (data from Han et al [8]). +, Positive; −, negative; w, weakly positive; nd, not detected.…”

Section: Amphritea Pacifica Rp18w (Jaffzp000000000)mentioning

confidence: 99%

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Bowmanella yangjiangensis sp. nov. and Amphritea pacifica sp. nov., isolated from mariculture fishponds in China

Cai

Lü

Qian

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Four Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strains (Y26, Y57T, ZJ14WT and RP18W) were isolated from mariculture fishponds in PR China. Comparisons based on 16S rRNA gene sequences showed that strains Y26 and Y57T share 16S rRNA gene sequence similarities in the range of 95.1−98.5 % with species of the genus Bowmanella , and strains ZJ14WT and RP18W share 16S rRNA gene sequence similarities in the range of 96.7 −98.8 % with species of the genus Amphritea , respectively. The genome sizes of strains Y26, Y57T, ZJ14WT and RP18W were about 4.85, 5.40, 4.70 and 4.70 Mbp with 49.5, 51.7, 51.2 and 51.3 mol% G+C content, respectively. The calculated pairwise OrthoANIu values among strains Y26, Y57T and species of the genus Bowmanella were in the range of 72.6−83.1 %, but the value between strains Y26 and Y57T was 96.2 %. The pairwise OrthoANIu values among strains ZJ14WT, RP18W and other species of the genus Amphritea were all less than 93.9 %, but the value between strains ZJ14WT and RP18W was 99.3 %. Q-8 was the major respiratory quinone of strains Y26, Y57T, ZJ14WT and RP18W, and the major fatty acids of these strains were all C16 : 1 ω7c, C16 : 0 and C18 : 1 ω7c. The predominant polar lipids of strains Y26 and Y57T included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol, but strains ZJ14WT and RP18W only contained phosphatidylethanolamine and phosphatidylglycerol. Combining phenotypic, biochemical and genotypic characteristics, strains Y26 and Y57T should belong to the same species and represent a novel member of the genus Bowmanella , and strains ZJ14WT and RP18W should belong to the same species and represent a novel member of the genus Amphritea , for which the names Bowmanella yangjiangensis sp. nov. (type strain Y57T=GDMCC 1.2180T=KCTC 82439T) and Amphritea pacifica sp. nov. (type strain ZJ14WT=GDMCC 1.2203T=KCTC 82438T) are proposed.

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Section: Full-textmentioning

confidence: 67%

Section: Full-textmentioning

confidence: 99%

Section: Amphritea Pacifica Rp18w (Jaffzp000000000)mentioning

confidence: 99%

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Bowmanella yangjiangensis sp. nov. and Amphritea pacifica sp. nov., isolated from mariculture fishponds in China

Cai

Lü

Qian

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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“…The results demonstrate the use of the novel ω-TA and the process development to produce a chiral amine, for which synthetic challenges have remained unsolved thus far. Coscolín et al first reported the wild-type class III (S)-selective TR 8 [10], which was isolated from a marine sample and most likely derived from a bacterium of the Amphritea genus that had not been previously reported to contain transaminases [35][36][37]. The enzyme was shown to be most active from 60 to 65 • C, being thermoactive up to 65 • C, to retain more than 35% activity in the presence of 50% (v/v) methanol, acetonitrile, or DMSO, and to prefer bulky ketones and (S)-amines [10].…”

Section: ]mentioning

confidence: 99%

“…Most commercially useful ketones, including S, exhibit low water solubility, which makes the amine acceptor inaccessible to a biocatalyst. This problem can be circumvented by using an organic solvent (a co-solvent) as a second phase or as reaction medium [17,35]. However, the addition of organic solvents to the reaction media can negatively affect en- No inhibition effects on the enzyme activity were observed at the tested concentrations of 4 -(trifluoromethyl)acetophenone (S), up to a concentration of 200 mM (Figure 1).…”

Section: Bioconversion Conditionsmentioning

confidence: 99%

ω-Transaminase-Mediated Asymmetric Synthesis of (S)-1-(4-Trifluoromethylphenyl)Ethylamine

2021

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The pivotal role played by ω-transaminases (ω-TAs) in the synthesis of chiral amines used as building blocks for drugs and pharmaceuticals is widely recognized. However, chiral bulky amines are challenging to produce. Herein, a ω-TA (TR8) from a marine bacterium was used to synthesize a fluorine chiral amine from a bulky ketone. An analysis of the reaction conditions for process development showed that isopropylamine concentrations above 75 mM had an inhibitory effect on the enzyme. Five different organic solvents were investigated as co-solvents for the ketone (the amine acceptor), among which 25–30% (v/v) dimethyl sulfoxide (DMSO) produced the highest enzyme activity. The reaction reached equilibrium after 18 h at 30% of conversion. An in situ product removal (ISPR) approach using an aqueous organic two-phase system was tested to mitigate product inhibition. However, the enzyme activity initially decreased because the ketone substrate preferentially partitioned into the organic phase, n-hexadecane. Consequently, DMSO was added to the system to increase substrate mass transfer without affecting the ability of the organic phase to prevent inhibition of the enzyme activity by the product. Thus, the enzyme reaction was maintained, and the product amount was increased for a 62 h reaction time. The investigated ω-TA can be used in the bioconversion of bulky ketones to chiral amines for future bioprocess applications.

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Marinomonas flavescens sp. nov., isolated from seawater adjacent to Fildes Peninsula, Antarctica

Zhai

Zhang

et al. 2019

International Journal of Systematic and Evolutionary Microbiology

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Amphritea opalescens sp. nov., isolated from sediment adjacent to Fildes Peninsula, Antarctica

Cited by 8 publications

References 19 publications

Bowmanella yangjiangensis sp. nov. and Amphritea pacifica sp. nov., isolated from mariculture fishponds in China

Bowmanella yangjiangensis sp. nov. and Amphritea pacifica sp. nov., isolated from mariculture fishponds in China

ω-Transaminase-Mediated Asymmetric Synthesis of (S)-1-(4-Trifluoromethylphenyl)Ethylamine

Marinomonas flavescens sp. nov., isolated from seawater adjacent to Fildes Peninsula, Antarctica

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