Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae)

Oliveira, Márcio Leite de; Duarte, José Maurício Barbanti

doi:10.4238/2013.january.16.8

Cited by 10 publications

(5 citation statements)

References 31 publications

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“…In order to avoid genotyping errors, several replicates should be extracted and compared [17][18][19]. In two studies using field-collected feces from mammals, one-third of the samples were successfully amplified [20,21]. Small mammals' hairs, when freshly collected (i.e., regularly visited hair traps), provided accurate genotyping and were as amplifiable and informative as liver samples [22].…”

Section: Introductionmentioning

confidence: 99%

Shall the Wild Boar Pass? A Genetically Assessed Ecological Corridor in the Geneva Region

et al. 2022

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Landscape fragmentation caused by road infrastructures represents a major threat to the genetic diversity of a region. The resulting genetic isolation between subpopulations may lead to consanguinity, and consequently to population collapse and extinction. However, the construction of wildlife crossings can help maintain connectivity. In the present paper, we evaluated the genetic spatial structuring of populations of wild boars (Sus scrofa) in three areas of the Geneva region connected by an ecological corridor. Those areas are cut off either by a highway that is crossed by a wildlife overpass or by an anthropized sector. Genetic profiling with 9 nuclear microsatellite markers yielded 61 single profiles, which allowed for clustering, parentage, and linkage disequilibrium analyses, uncovering the populations’ genetic structure. We also evaluated whether the genetic structure was affected by the sex of individuals. In our analyses, all individuals clustered into a single genetic group, suggesting that no structure limited significantly the gene flow in the region. However, a recent admixture indicated a potential increase in the gene flow between two of the subpopulations due to the wildlife overpass, while the other part of the ecological corridor was not or was only partially functional. Genetic distances between males were significantly higher than between females, although the role of sex remains unclear as to its influence on population genetics. Finally, in order to avoid a subregion becoming fully isolated, urbanization planning should consider this genetic evaluation and proceed with further monitoring, especially by focusing on species more sensitive to landscape fragmentation.

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Section: Introductionmentioning

confidence: 99%

Shall the Wild Boar Pass? A Genetically Assessed Ecological Corridor in the Geneva Region

et al. 2022

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“…The amplification of at least one microsatellite locus in all the samples and 5-6 loci in ~70% of the samples analyzed indicated a high rate of success for fecal DNA. These results indicate that the use of fresh fecal samples can enhance the success of studies using fecal DNA samples ( Oliveira and Duarte, 2013 ). In contrast, based on samples collected in northern Chile during the summer, Espinosa et al (2015) concluded that fresh feces (based on the presence of mucus or deer observed defecating) and non-fresh feces (no mucus, deer not observed defecating) were equally efficient for successful DNA amplification in ungulate species.…”

Section: Discussionmentioning

confidence: 96%

“…Since some studies have shown that the success in amplification varies according to locus size ( e.g. Oliveira and Duarte, 2013 ), we also investigated the correlation between these variables by calculating Pearson's correlation coefficient.…”

Section: Methodsmentioning

confidence: 99%

Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci

et al. 2017

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The pampas deer (Ozotoceros bezoarticus) is close to being classified as ‘globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability.

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“…For each scat found by the searching team, we transferred a 2-3 cm section to a DNA-free sample container (Sarstedt, Mawson Lakes, AUS) with 30 ml pure ethanol. Where mucus was still existent on the scat surface, it was sampled to increase the probability of successful DNA analyses (Oliveira and Duarte 2013). We stored samples protected from high temperatures and sunlight until further processing.…”

Section: Scat Detection Dogmentioning

confidence: 99%

Eyes, ears, or nose? Comparison of three non-invasive methods to survey wolf recolonisation

Cozzi

Hollerbach

Suter³

et al. 2021

Mamm Biol

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The development and use of cost-effective and appropriate survey methods to assess species distribution and to monitor range expansion and contraction of wild populations is crucial due to the limited financial resources for conservation. Of particular importance, yet little studied, is the ability to collect information before a wild population is well established, i.e. at the early stages of recolonisation. During 2018 and 2019, we used camera traps, audio recorders, and scat detection dogs simultaneously to investigate composition, detection probability, and territorial extent of a pack of wolves in the Swiss Alps. We compared the efficacy of these survey methods by assessing sampling effort, data obtained, and costs. We show that, under the presented setup, camera traps and scat detection dogs substantially outperformed audio recorders in detecting wolves, representing the packs’ territorial extent, and revealing the number of adult wolves. The detection dogs did not detect pups but, unlike the other methods, allowed the identification of single individuals. The use of four camera traps during 13 weeks, a 24-km-long transect walked with the detection dog, or the use of one audio recorder during 148 weeks were necessary to obtain a comparable wolf detection probability. Our results show that no single method was able to return all information that we hoped to collect. Comprehensive and cost-effective information was best obtained by combining data from camera traps and detection dogs. We suggest both methods to be simultaneously used to successfully investigate wolf recolonisation into historical range.

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Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae)

Cited by 10 publications

References 31 publications

Shall the Wild Boar Pass? A Genetically Assessed Ecological Corridor in the Geneva Region

Shall the Wild Boar Pass? A Genetically Assessed Ecological Corridor in the Geneva Region

Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci

Eyes, ears, or nose? Comparison of three non-invasive methods to survey wolf recolonisation

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