Amplification and characterization of an inverted repeat from the Chlamydomonas reinhardtii mitochondrial genome

Ma, Ding; King, Yueh-Tsu; Kim, Yong; Luckett, William; Boyle, John A.; Chang, Yung-Fu

doi:10.1016/0378-1119(92)90279-x

Cited by 17 publications

(11 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These three sequences may be necessary for the complete replication of the ends via a circular intermediate (Vahrenholz et al 1993). The presence of rare circular molecules in samples prepared for electron microscopy (Ryan et al 1978) and the isolation of a PCR product that may have arisen from the use of a circular mt DNA as a template (Ma et al 1992) provide some support for this hypothesis.…”

Section: Introductionmentioning

confidence: 68%

Structure of the telomeric ends of mt DNA, transcriptional analysis and complex I assembly in the dum24 mitochondrial mutant of Chlamydomonas reinhardtii

et al. 2001

View full text Add to dashboard Cite

The dum24 mutant of Chlamydomonas reinhardtii contains four types of altered mitochondrial linear genomes: two types of deleted monomers and two types of dimers resulting from fusions between some monomers via their deleted ends. All molecules lack at least cob, nd4 and the 3' end of nd5, three adjacent genes located in the left part of the genome. We present evidence showing that in dum24, as in other deletion mutants, the deletions extend to the left telomeric end, and propose that the only replicative forms in the mutants are the dimeric DNA molecules that possess intact telomeric structures at both ends. Two abnormally large transcripts produced from chimeric genes are detected in dum24, which throws some light on the location of potential promoter sequences and processing signals in the mitochondrial genome. Using BN-PAGE analysis and immunological methods to detect complex I, we further show that dum24 mitochondria do not possess the normal multimeric complex I (850 kDa), but produce a smaller, partially assembled, complex (650 kDa), demonstrating a role for ND4 and/or ND5 subunits(s) in complex I assembly.

show abstract

Section: Introductionmentioning

confidence: 68%

Structure of the telomeric ends of mt DNA, transcriptional analysis and complex I assembly in the dum24 mitochondrial mutant of Chlamydomonas reinhardtii

et al. 2001

View full text Add to dashboard Cite

show abstract

“…moewusii. Similarly, no remnants of the inverted repeats characteristic of the linear C. reinhardtii mt genome (Ma et al 1992;Vahrenholz et al 1993) were found in C. elongatum. Hence, the same explanation might apply to them.…”

Section: Discussionmentioning

confidence: 97%

“…In Southern blots from restricted and electrophoretically separated organellar DNA probed with fragments from different sites of the mtDNA, only a single DNA species of about 23 kb could be detected in these digests (data not shown). Furthermore, no regions homologous to the C. reinhardtii inverted repeats (Ma et al 1992;Vahrenholz et al 1993) were found. These results are consistent with a circular conformation, as shown in Fig.…”

Section: Genome Structurementioning

confidence: 98%

“…smithii and C. eugametos/ C. moewusii display quite different structural features. The 15.8-kilo base pair (kb) mitochondrial genome from C. reinhardtii is linear (Ryan et al 1978;Michaelis et al 1990;Harris 1993) and flanked by characteristic inverted repeats with 3Ј single-stranded, noncomplementary extensions of 39 to 41 nucleotides (Ma et al 1992;Vahrenholz et al 1993). Both DNA strands encode genes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Mitochondrial Genome of Chlorogonium elongatum Inferred from the Complete Sequence

Kroymann

Zetsche

1998

J Mol Evol

View full text Add to dashboard Cite

The 22,704-bp circular mitochondrial DNA (mtDNA) of the chlamydomonad alga Chlorogonium elongatum was completely cloned and sequenced. The genome encodes seven proteins of the respiratory electron transport chain, subunit 1 of the cytochrome oxidase complex (cox1), apocytochrome b (cob), five subunits of the NADH dehydrogenase complex (nad1, nad2, nad4, nad5, and nad6), a set of three tRNAs (Q, W, M), and the large (LSU)- and small (SSU)-subunit ribosomal RNAs. Six group-I introns were found, two each in the cox1, cob, and nad5 genes. In each intron an open reading frame (ORF) related to maturases or endonucleases was identified. Both the LSU and the SSU rRNA genes are split into fragments intermingled with each other and with other genes. Although the average A + T content is 62.2%, GC-rich clusters were detected in intergenic regions, in variable domains of the rRNA genes, and in introns and intron-encoded ORFs. A comparison of the genome maps reveals that C. elongatum and Chlamydomonas eugametos mtDNAs are more closely related to one another than either is to Chlamydomonas reinhardtii mtDNA.

show abstract

“…7B and data not shown) that were rapidly lost by culturing the parasite in the absence of drug selection for ϳ10 passages. Chromosomal instability is often associated, among other things, with gene amplification (55,56). These results indicate that specific parts of Leishmania-truncated minichromosomes demonstrate a high mitotic stability that ensures their retention and segregation during cell division suggesting the presence of "centromere-like" sequences within these chromosomes.…”

Section: Mitotic Stability Of Truncated Chromosomes In Leishmaniamentioning

confidence: 92%

A Telomere-mediated Chromosome Fragmentation Approach to Assess Mitotic Stability and Ploidy Alterations of LeishmaniaChromosomes

Tamar

Papadopoulou

2001

Journal of Biological Chemistry

View full text Add to dashboard Cite

We have used a telomere-associated chromosome fragmentation strategy to induce internal chromosome-specific breakage of Leishmania chromosomes. The integration of telomeric repeats from the kinetoplastid Trypanosoma brucei into defined positions of the Leishmania genome by homologous recombination can induce chromosome breakage accompanied by the deletion of the chromosomal part that is distal to the site of the break. The cloned telomeric DNA at the end of the truncated chromosomes is functional and it can seed the formation of new telomeric repeats. We found that genome ploidy is often altered upon telomere-mediated chromosome fragmentation events resulting in large chromosomal deletions. In most cases diploidy is either preserved, or partial trisomic cells are observed, but interestingly we report here the generation of partial haploid mutants in this diploid organism. Partial haploid Leishmania mutants should facilitate studies on the function of chromosome-assigned genes. We also present several lines of evidence for the presence of sequences involved in chromosome mitotic stability and segregation during cell cycle in this parasitic protozoan.

show abstract

Amplification and characterization of an inverted repeat from the Chlamydomonas reinhardtii mitochondrial genome

Cited by 17 publications

References 31 publications

Structure of the telomeric ends of mt DNA, transcriptional analysis and complex I assembly in the dum24 mitochondrial mutant of Chlamydomonas reinhardtii

Structure of the telomeric ends of mt DNA, transcriptional analysis and complex I assembly in the dum24 mitochondrial mutant of Chlamydomonas reinhardtii

The Mitochondrial Genome of Chlorogonium elongatum Inferred from the Complete Sequence

A Telomere-mediated Chromosome Fragmentation Approach to Assess Mitotic Stability and Ploidy Alterations of LeishmaniaChromosomes

Contact Info

Product

Resources

About