Amplification of altered self-reactive cytolytic T lymphocyte responses by cloned, allospecific human Th cells.

Friedman, Steven; Green, A; Russo, Carlo; Posnett, David N.; Diffley, D; Crow, Mary K.

doi:10.1172/jci113786

Cited by 2 publications

(4 citation statements)

References 35 publications

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“…It is of interest that one of the T cell clones we used, clone 86, makes IL-4 but not IL-2 (21) . Supernatants from clone:APC cocultures fail to induce growth ofthe IL-2 dependent line CTLL but can enhance CD23 (Foe receptor) expression on B cells (21), a property determined to be dependent upon IL-4 (22). Clone 86 is as efficient as fresh, alloreactive T blasts in inducing IL-1, but it still proliferates in the presence of neutralizing anti-IL-1.…”

Section: Discussionmentioning

confidence: 99%

“…In the mouse, IL-1 can be essential for (19), or might enhance (20), the growth of IL-4-dependent T cell lines. It is of interest that one of the T cell clones we used, clone 86, makes IL-4 but not IL-2 (21) . Supernatants from clone:APC cocultures fail to induce growth ofthe IL-2 dependent line CTLL but can enhance CD23 (Foe receptor) expression on B cells (21), a property determined to be dependent upon IL-4 (22).…”

Section: Discussionmentioning

confidence: 99%

“…T. Yokota) ; rTNF 25 ng/ml (kindly provided by Dr. Helen Vlassara, Rockefeller University, New York), rIL-6, 500 U/ml (DNAX) . To evaluate the capacity of T cells to induce monocyte or dendritic cell IL-1, 5 x 10' enriched dendritic cells or 5 x 10 4 monocytes were mixed with 1 .5 x 106 syngeneic or allogeneic blood T cells for [18][19][20][21][22][23][24] h . Syngeneic cultures were stimulated with Con A (3 ug/ml) or OKT3 (25 ng/ml) .…”

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confidence: 99%

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Interleukin 1 production during accessory cell-dependent mitogenesis of T lymphocytes.

Bhardwaj

Lau

Friedman

et al. 1989

The Journal of Experimental Medicine

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The concept that IL-1 is produced and is an essential cofactor for T cell proliferative responses to antigens has not been extensively explored with fresh populations of mononuclear cells. Recent studies in the mouse, with an IL-lot cDNA probe and rIL-1 have indicated that IL-1 is produced during monocyte but not dendritic cellmediated, T cell proliferation (1). Furthermore, the activating properties of exogenous rIL-1 may be exerted at the level ofthe dendritic cell rather than the T cell (2).We have studied IL-1 production during the human APCT cell interaction. The human system is advantageous since one has access to monocytes and dendritic cells in blood, as well as a useful group ofanti-IL-1 antibodies and antigen-specific clones . We find that IL-1 can be produced, but may not be essential for secondary T cell responses to antigen-bearing monocytes, and that there are MHC-restricted and nonrestricted pathways whereby T cells can induce IL-1 production by monocytes. Volume 169 March 1989 1121-1136 Materials and Methods Culture Medium. Complete medium was RPMI 1640 (Gibco Laboratories, Grand Island, NY) supplemented with 5 x 10 -5 M 2-ME, 20 hg/ml gentamycin (Gibco Laboratories), and 5-10% FCS (HyClone Laboratories, Logan, UT) or human AB`serum. FCS was used for OKT3 (anti-CD3)-induced T cell proliferative responses while AB`serum was used in all other circumstances .Enrichment of Blood Mononuclear Cells, Monocytes, Dendritic Cells, and T Cells . Whole blood or human leukocyte fractions (New York Blood Center, New York, NY) were layered onto Ficoll Hypaque (Histopaque; Sigma Chemical Co., St. Louis, MO) and sedimented at 1,000 g for 20 min at 21°C . The mononuclear cells were washed twice in RPMI and studied immediately, or after 1-2 d in culture. Monocytes were enriched by adherence for 90 min at 37°C to plastic. The adherent cells were 55-85% monocytes by cytology and immunolabeling with the anti-CD14 mAb 3C10 (3). This antibody stains a majority (>95%) of blood monocytes and does not stain T cells, B cells, or dendritic cells (3). When highly enriched dendritic cells were required these were separated by a Percoll sedimentation method described elsewhere (4). The dendritic cells were obtained from the high density fraction by a multistep procedure involving depletion of T cells by sheep erythrocyte (E) rosetting, B cells by a density gradient, and residual monocytes by panning on dishes coated with human gamma globulin .

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Interleukin 1 production during accessory cell-dependent mitogenesis of T lymphocytes.

Bhardwaj

Lau

Friedman

et al. 1989

The Journal of Experimental Medicine

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“…Assay ofSA-delx*ndent Cytdysis. TCL cells were assayed for cytolyric activity in a 4-h SlCr release assay (36), using MHC class II antigen-bearing target ceUs, B cell lymphoblastoid cell line 8866. Briefly, 8866 cells were incubated for 2 h at 37~ with SlCr in the presence of final medium alone, or the indicated SA.…”

Section: Generation Of Tcl Enriched For Specific Tcr V Gene Usagementioning

confidence: 99%

Characterization of human T cells reactive with the Mycoplasma arthritidis-derived superantigen (MAM): generation of a monoclonal antibody against V beta 17, the T cell receptor gene product expressed by a large fraction of MAM-reactive human T cells.

Friedman

Crow

Tumang

et al. 1991

The Journal of Experimental Medicine

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SummaryWhile all known microbial superantigens are mitogenic for human peripheral blood lymphocytes (PBL), the functional response induced by Mycoplasma arthritidis-derived superantigen (MAM) is unique in that/CLAM stimulation of PBL consistently results in T ceLl-dependent B cell activation characterized by polyclonal IgM and IgG production. These immunostimulatory effects of MAM on the humoral arm of the human immune system warranted a more precise characterization of MAM-reactive human T cells. Using an undoned MAM reactive human T cell line as immunogen, we have generated a monoclonal antibody (mAb) (termed C1) specific for the T cell receptor VB gene expressed by the major fraction of MAM-reactive human T cells, VB17. In addition, a VB17-MAM-reactive T cell population exists, assessed by MAM, induced T cell proliferation and cytotoxic T cell activity, mAb C1 will be useful in characterizing the functional properties of V~17 + T cells and their potential role in autoimmune disease.

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Amplification of altered self-reactive cytolytic T lymphocyte responses by cloned, allospecific human Th cells.

Abstract: The effect of a cloned allospecific human Th cell, termed 86

Cited by 2 publications

References 35 publications

Interleukin 1 production during accessory cell-dependent mitogenesis of T lymphocytes.

Interleukin 1 production during accessory cell-dependent mitogenesis of T lymphocytes.

Characterization of human T cells reactive with the Mycoplasma arthritidis-derived superantigen (MAM): generation of a monoclonal antibody against V beta 17, the T cell receptor gene product expressed by a large fraction of MAM-reactive human T cells.

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