Amplification of human papillomavirus DNA sequences by using conserved primers

Grégoire, Lucie; Arella, M; Campione-Piccardo, José; Lancaster, Wayne D.

doi:10.1128/jcm.27.12.2660-2665.1989

Cited by 99 publications

(28 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To efficiently identify HPV 16 variants in low-copy number HPV DNA, nested PCR was performed. The conditions used were as previously described (14,15) .…”

Section: Detecting Hpv 16 Infection By Polymerase Chain Reactionmentioning

confidence: 99%

Polymorphism in theE6gene of human papillomavirus type 16 in the cervical tissues of Korean women

Kang¹,

Jeon²,

Kim³

et al. 2005

Int J Gynecol Cancer

View full text Add to dashboard Cite

The aim of this study was to identify sequence variants in the HPV 16 E6 gene in Korean women and to examine the possible association between these sequence variants and cervical cancer development. We examined the HPV 16 DNA of 215 patients with no cervical disease (NCD) (n = 105) or with cervical neoplasia (n = 110) [cervical intraepithelial neoplasia (CIN), n = 61; invasive cervical carcinoma (ICC), n = 49] using the nested polymerase chain reaction (PCR) and PCR-directed sequencing methods. Fifty-four (NCD, n = 10; CIN, n = 17; ICC, n = 27) of the 215 samples contained HPV 16 E6 DNA, but only two (7.4%) of 27 ICC samples had prototype sequences. The most frequently found variation was D25E (in NCD, n = 8, 80%; in CIN, n = 9, 52.9%; in ICC, n = 23, 85.2%). This is a rare variation in western countries. No significance difference was found between the frequencies of D25E variation in cancerous and non-cancerous lesions. Among the 11 kinds of variants identified, four variants were novel and have been registered with GenBank. This study demonstrates that the D25 variant is the most prevalent E6 genomic variant type in Korean population. However, it was not found to be associated with an increased risk of ICC.

show abstract

“…To efficiently identify HPV 16 variants in low-copy number HPV DNA, nested PCR was performed. The conditions used were as previously described (14,15) .…”

Section: Detecting Hpv 16 Infection By Polymerase Chain Reactionmentioning

confidence: 99%

Polymorphism in theE6gene of human papillomavirus type 16 in the cervical tissues of Korean women

Kang¹,

Jeon²,

Kim³

et al. 2005

Int J Gynecol Cancer

View full text Add to dashboard Cite

show abstract

“…Surprisingly no evidence of HPV DNA was found. 6,7 According to the clinical and histological data, the diagnosis of AN with florid cutaneous and mucosal papillomatosis was made. This type of AN is frequently associated with internal malignancy; therefore, we set up instrumental and serological exams to discover it.…”

Section: Case Reportmentioning

confidence: 99%

Florid cutaneous and mucosal papillomatosis with acanthosis nigricans revealing a primary lung cancer

Bottoni

Dianzani

Pranteda

et al. 2000

Acad Dermatol Venereol

View full text Add to dashboard Cite

This is the report of an 80-year-old patient with diffuse brownish hyperpigmentation and velvety thickening of the skin with onset 1 year before. Warty lesions on his limbs were present as well as papillomatous and verrucous lesions on his lips, mouth and eyelid conjunctivae with hyperkeratosis of the nipples. Biopsies, performed at different sites, showed histological pictures consistent with a diagnosis of acanthosis nigricans (AN) with florid cutaneous and mucosal papillomatosis. This type of AN is frequently associated with internal malignancy. In our patient serum levels of tissue polypeptide antigen, carcinoembryonic antigen, cytokeratin fragment and squamous cell carcinoma antigen were high and chest computed tomography scan indicated a large tumour infiltrating the right lung and extending to the mediastinum. Cytological examination of bronchial drainage revealed the presence of neoplastic cells, non-small cell type carcinoma. The most frequent cancer associated with malignant AN is gastric adenocarcinoma. Lung tumour has rarely been reported with AN. Malignant AN is sometimes associated with other cutaneous and mucosal warty lesions, as in our patient. These various skin and mucosal lesions are the expression of a systemic epithelial disorder and may help clinicians to suspect a malignant form of AN.

show abstract

“…Several polymerase chain reaction (PCR) assays have been developed for sensitive and rapid detection of HPV DNA in cervical scrapes [for review see Walboomers et al, 19941. In the past, consensus or general primer mediated PCRs (GP-PCR) were applied for detection of a broad spectrum of genital HPVs by a single PCR assay [Manos et al, 1989;Gregoire et al, 1989;Snijders et al, 1990;De Roda Husman et al, 19951. Subsequent typing into individual HPVs could then be carried out in several ways.…”

Section: Introductionmentioning

confidence: 99%

A non-radioactive PCR enzyme-immunoassay enables a rapid identification of HPV 16 and 18 in cervical scrapes after GP5+/6+ PCR

et al. 1996

View full text Add to dashboard Cite

In previous studies, general primer mediated PCR (GP5+/6+ PCR) was applied successfully to detect a broad spectrum of human papillomaviruses (HPV) in cervical scrapes. In order to facilitate PCR based HPV detection and typing, a colourimetric microtitre plate based hybridisation assay was developed. The method utilised one biotinylated primer (bio-GP6+) in the GP-PCR. Biotinylated PCR products were captured on streptavidin coated microtitre plates, denaturated and hybridised to digoxigenin (DIG) labelled HPV specific internal oligo probes. The DIG labelled hybrids were detected using an enzyme immunoassay (EIA). Since HPV 16 and 18 are the most common HPV types found in cervical carcinomas, this approach was initiated for these two types. Cross-hybridisation reactions were not detected when the specificity of this PCR-EIA for HPV 16 and 18 was tested on a panel of 20 different HPV genotypes. The sensitivity of the assay was found to be between 10 and 100 HPV 16 and 18 viral genomes in a background of 100 ng cellular DNA. This was similar to the detection limit of Southern blot analysis of PCR products with radioactively labelled oligonucleotides. A group of cytomorphologically normal (n = 89) and abnormal (n = 96) cervical scrapes were composed of HPV 16 and HPV 18 positive and HPV negative scrapes. All HPV 16 and 18 positive smears were detected by PCR-EIA. These results indicate that PCR-EIA has the potential for a rapid and sensitive HPV DNA test for day-to-day routine examination of cervical scrapes.

show abstract

Amplification of human papillomavirus DNA sequences by using conserved primers

Cited by 99 publications

References 36 publications

Polymorphism in theE6gene of human papillomavirus type 16 in the cervical tissues of Korean women

Polymorphism in theE6gene of human papillomavirus type 16 in the cervical tissues of Korean women

Florid cutaneous and mucosal papillomatosis with acanthosis nigricans revealing a primary lung cancer

A non-radioactive PCR enzyme-immunoassay enables a rapid identification of HPV 16 and 18 in cervical scrapes after GP5+/6+ PCR

Contact Info

Product

Resources

About