Amplification of Immunostaining Intensity by the Peroxidase-Antiperoxidase, Avidin-Biotin-Peroxidase Complex or Catalyzed Signal Amplification Method Gives Erroneous Antigen Content in Sections.

Watanabe, Jun; Mondo, Hiroko; Takeda, Kazuo; Takamori, Yasuharu; Kanamura, Shinsuke

doi:10.1267/ahc.32.407

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…Serial sections were incubated with monoclonal antibodies to CEA (Zymed Labs Inc., CA, USA; dilution × 500), CA19‐9 (Zymed Labs Inc.; dilution × 300), DU‐PAN‐2 (Kyowa Medecs, Tokyo, Japan; dilution × 500), and PCNA (Chemicon Int. Inc., CA, USA; dilution × 500) at 4°C for 24 h and they were treated at 4°C for 1 h with biotinylated anti‐mouse IgG (Vector Labs, Burlingame, CA, USA; dilution × 50) followed by avidin–biotin–peroxidase complex (Vectastain ABC kit; Vector Labs; dilution × 50) at 4°C for 30 min 24 , 25 . They were stained with 0.001% diaminobenzidine solution containing hydrogen peroxide at 20°C for 15 min and counterstained with haematoxylin.…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemical localization of CEA, CA19‐9 and DU‐PAN‐2 in hepatitis C virus‐infected liver tissues

et al. 2002

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CEA in chronic hepatitis and liver cirrhosis is expressed not only in bile ductules but also on membrane facing bile canaliculi, and both CA19-9 and DU-PAN-2 were seen in different levels of biliary ducts. These molecules were expressed in bile ductules in surrounding non-neoplastic areas of hepatocellular carcinoma, and their expression was not associated with regeneration of biliary ducts. CEA expression was present in the trabecular type of hepatocellular carcinoma, and CA19-9 and DU-PAN-2 were observed in cholangiolar areas in mixed type of hepatocellular carcinoma.

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Section: Methodsmentioning

confidence: 99%

Immunohistochemical localization of CEA, CA19‐9 and DU‐PAN‐2 in hepatitis C virus‐infected liver tissues

et al. 2002

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Method for synthesizing ferrite nanoparticles ∼30 nm in diameter on neutral pH condition for biomedical applications

Tada

Hatanaka

Sanbonsugi

et al. 2003

Journal of Applied Physics

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Nanosized ferrite spherical particles, ∼30 nm in diameter as revealed by scanning electron microscopy, were synthesized from an aqueous Fe(OH)2 suspension (pH=7.6–8.0) at 25 °C by oxidizing it with H2O2. The nanoparticles were of a spinel structure of an intermediate between Fe3O4 and γ-Fe2O3, as revealed by x-ray diffraction. Compared to the nanoparticles synthesized by our previous method in which an aqueous solution of Fe2++Fe3+ was oxidized by air (oxygen), the nanoparticles increased in size, from ∼10 nm (previous method) to 30 nm. Also saturation magnetization increased, though slightly, from 76 emu/g (previous method) to 80 emu/g (present method). Therefore, the ferrite nanoparticles synthesized by this method will improve the efficiency of magnetic separation. Because synthesis is performed at room temperature and neutral conditions (pH=7.1–7.8), which are compatible with most bioactive molecules (e.g., antibodies and proteins), these molecules will be immobilized onto the surface of the nanoparticles during their syntheses.

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Amplification of Immunostaining Intensity by the Peroxidase-Antiperoxidase, Avidin-Biotin-Peroxidase Complex or Catalyzed Signal Amplification Method Gives Erroneous Antigen Content in Sections.

Cited by 2 publications

References 18 publications

Immunohistochemical localization of CEA, CA19‐9 and DU‐PAN‐2 in hepatitis C virus‐infected liver tissues

Immunohistochemical localization of CEA, CA19‐9 and DU‐PAN‐2 in hepatitis C virus‐infected liver tissues

Method for synthesizing ferrite nanoparticles ∼30 nm in diameter on neutral pH condition for biomedical applications

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