Amplification Techniques for Optical Detection

Griffin, Guy D.; Williams, M. W.; Stratis‐Cullum, Dimitra N.; Vo‐Dinh, Tuan

doi:10.1201/9780203008997.ch55

Cited by 3 publications

(2 citation statements)

References 296 publications

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“…Many applications rely on biological recognition as a means of achieving specificity for a particular target species (1)(2)(3)(4)(5). Although antibodies are most commonly employed, aptamers are another class of affinity probe and they possess a number of advantages over traditional bioreceptors (4,(6)(7)(8)(9).…”

Section: Inroductionmentioning

confidence: 99%

Affinity Probe Capillary Electrophoresis Evaluation of Aptamer Binding to Campylobacter jejuni Bacteria

Stratis‐Cullum¹,

McMasters²,

Pellegrino³

2009

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Section: Inroductionmentioning

confidence: 99%

Affinity Probe Capillary Electrophoresis Evaluation of Aptamer Binding to Campylobacter jejuni Bacteria

Stratis‐Cullum¹,

McMasters²,

Pellegrino³

2009

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“…Since the mid-1990s, bioanalytical systems have greatly enhanced our level of understanding toward the development of detection and monitoring devices for various biological sensitive elements [1]. Higher percentages of research documents have been published on detection instruments; however, close-to-ideal sensing systems emerged only with the development of biosenors [1][2][3][4], which use a highly specific to the target analyte biorecognition element attached to the transducer for providing sensitive read-out signal corresponding to the concentration of the analyte. It is possible to categorize biosensors in different ways based on the physicochemical transducers, which include electrochemical [5], optical [6], piezoelectric [7], microgravimetric [8], magnetic [9], mass sensitive [10,11] and thermal [12], or according to the biorecognition elements based catalytic or affinity mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Novel trends in affinity biosensors: current challenges and perspectives

Arugula¹,

Simonian²

2014

Meas. Sci. Technol.

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Molecular biorecognition processes facilitate physical and biochemical interactions between molecules in all crucial metabolic pathways. Perhaps the target analyte and the biorecognition element interactions have the most impactful use in biosensing applications. Traditional analytical sensing systems offer excellent biorecognition elements with the ability to detect and determine the presence of analytes. High affinity antibodies and DNA play an important role in the development of affinity biosensors based on electrochemical, optical and mass sensitive approaches. Advancements in this area routinely employ labels, label free, nanoparticles, multifunctional matrices, carbon nanotubes and other methods to meet the requirements of its own application. However, despite increasing affinity ceilings for conventional biosensors, the field draws back in meeting specifically important demands, such as long-term stability, ultrasensitivity, rapid detection, extreme selectivity, strong biological base, calibration, in vivo measurements, regeneration, satisfactory performance and ease of production. Nevertheless, recent efforts through this line have produced novel high-tech nanosensing systems such as ‘aptamers’ and ‘phages’ which exhibit high-throughput sensing. Aptamers and phages are powerful tools that excel over antibodies in sensibility, stability, multi-detection, in vivo measurements and regeneration. Phages are superior in stability, screening for affinity-based target molecules ranging from small to proteins and even cells, and easy production. In this review, we focus mainly on recent developments in affinity-based biosensors such as immunosensors, DNA sensors, emphasizing aptasensors and phage-based biosensors basing on novel electrochemical, optical and mass sensitive detection techniques. We also address enzyme inhibition-based biosensors and the current problems associated with the above sensors and their future perspectives.

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Intensified biochip system using chemiluminescence for the detection of Bacillus globigii spores

et al. 2008

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This paper reports the first intensified biochip system for chemiluminescence detection and the feasibility of using this system for the analysis of biological warfare agents is demonstrated. An enzyme-linked immunosorbent assay targeting Bacillus globigii spores, a surrogate species for Bacillus anthracis, using a chemiluminescent alkaline phosphatase substrate is combined with a compact intensified biochip detection system. The enzymatic amplification was found to be an attractive method for detection of low spore concentrations when combined with the intensified biochip device. This system was capable of detecting approximately 1×105 Bacillus globigii spores. Moreover, the chemiluminescence method, combined with the selfcontained biochip design, allows for a simple, compact system that does not require laser excitation and is readily adaptable to field use.

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Amplification Techniques for Optical Detection

Cited by 3 publications

References 296 publications

Affinity Probe Capillary Electrophoresis Evaluation of Aptamer Binding to Campylobacter jejuni Bacteria

Affinity Probe Capillary Electrophoresis Evaluation of Aptamer Binding to Campylobacter jejuni Bacteria

Novel trends in affinity biosensors: current challenges and perspectives

Intensified biochip system using chemiluminescence for the detection of Bacillus globigii spores

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