Amplified fluorescence detection of DNA based on catalyzed dynamic assembly and host–guest interaction between β-cyclodextrin polymer and pyrene

Recent Advances in Nucleic Acid Targeting Probes and Supramolecular Constructs Based on Pyrene-Modified Oligonucleotides

“…Due to the remarkable property of pyrene fluorophores to form excimers and exciplexes with pronounced changes in output fluorescence signals, pyrene-modified MBs have been well developed. To obtain MBs with improved emission characteristics and specificity, several interesting design decisions have been proposed (see, for instance, [ 29 , 30 , 66 , 85 , 92 , 94 , 95 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 ]; some of them are illustrated in Figure 9 ), among them are excimer-forming MBs containing a quencher (for instance, types MB1a [ 85 , 100 , 105 ], MB1b [ 105 ], MB1c and MB1d [ 100 ], and MB2 [ 30 ]) and numerous quencher-free MBs (for instance, types MB3 [ 66 ], MB4 [ 94 ], MB5 [ 29 , 101 ], MB6 [ 109 ], MB7 [ 106 , 108 ], and MB8 [ 107 ]), including systems that utilize γ-cyclodextrin or hybridization chain reaction-mediated signal amplification (types MB9 [ 95 , 111 ] and MB10 [ 104 ]).…”

“…That is why it can solve the issue of complex probe design and also provide further sensitivity improvements. These systems were used for DNA [ 111 , 146 , 150 ] and miRNA [ 149 , 151 ] detection. Multi-component systems for the detection of adenosine on the basis of aptamers and β-CDP were reported [ 144 , 146 ] ( Figure 21 ).…”

Highly selective detection of deoxyribonucleic acid in living cells using RecA‐green fluorescent protein‐single‐stranded deoxyribonucleic acid filament fluorescence resonance energy transfer probe

“…Therefore, the identification of specific gene mutation may help in predicting the severity of jaundice [2]. There are several methods for the detection of DNA, including spectrophotometry [3,4], surface-enhanced Raman scattering [5,6], electrochemical methods [7,8], fluorescence methods [9,10], and chemiluminescence [11]. Of these research methods, fluorescence methods have been widely recognized for their high sensitivity, easy readout, and simple operation [12].…”

A gold nanoparticle based colorimetric and fluorescent dual-channel probe for acetylcholinesterase detection and inhibitor screening

“…Supramolecular host-guest interaction has played an important role in the chemical sensing research, including the sensor chip for the metal ion detection 24,25 and the uorescent DNA detection. 26,27 The use of hostguest interactions is an attractive alternative to electrostatic interaction since the recognition motifs are specic and bioorthogonal without an additional catalyst, which is suitable for biological application.…”