Amplified fragment length polymorphism (AFLP) as suitable markers to study <i>orobanche cumana</i> genetic diversity

Gagné, Geneviève; Roeckel‐Drevet, Patricia; Grezes-Besset, Bruno; Shindrova, P.; Ivanov, P.; Grand-Ravel, Catherine; Vear, Félicity; Charmet, Gilles; Nicolas, P.

doi:10.1046/j.1439-0434.2000.00528.x

Cited by 16 publications

(13 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…, 1995) the marker of choice to study genetic diversity in different parasitic plant species like Striga asiatica (Botanga et al. , 2002), S. gesnerioides (Botanga & Timko, 2005) and O. cumana (Gagne et al. , 2000).…”

Section: Introductionmentioning

confidence: 99%

“…A higher capability of generating a large number of reproducible loci has made amplified fragment-length polymorphism (AFLP) (Vos et al, 1995) the marker of choice to study genetic diversity in different parasitic plant species like Striga asiatica (Botanga et al, 2002), S. gesnerioides (Botanga & Timko, 2005) and O. cumana (Gagne et al, 2000). Botanga and Timko (2005) could even identify marker fragments that discriminated between S. gesnerioides strains parasitic on Indigofera hirsuta L. and those parasitic on cowpea.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity of Moroccan populations of Orobanche foetida: evolving from parasitising wild hosts to crop plants

et al. 2008

View full text Add to dashboard Cite

Summary Orobanche foetida is widely distributed in western Mediterranean countries infecting wild legumes. Recently it has been reported in Morocco infecting common vetch, presenting a further constraint for legume production in this area. Comparative studies between Orobanche populations attacking wild species and Orobanche populations growing on crops from the same region may help to clarify whether a host specialisation process occurred. In the present study, the genetic variability of five O. foetida populations, four infecting wild plants and one infecting cultivated vetch in Morocco was characterised using amplified fragment‐length polymorphism (AFLP) markers to elucidate the existing genetic relationship between populations and to suggest a potential origin for the recently detected vetch‐infecting population. Analysis of molecular variance suggested the existence of genotypic differentiation among populations. The most genetic divergent population by cluster analysis was the population collected on Ornithopus sativus. The vetch‐infecting O. foetida population was closer to the three populations infecting Scorpiurus muricatus. The possibility of the presence of a reservoir of diversity is balanced against direct genetic adaptation and completely new introduction. The potential for this Orobanche species to shift host and become a threat to agriculture exists and has to be taken into account for future legume breeding for these areas.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Moroccan populations of Orobanche foetida: evolving from parasitising wild hosts to crop plants

et al. 2008

View full text Add to dashboard Cite

show abstract

“…As AFLP markers are distributed across the whole genome, they have a high multiplex ratio, meaning a large number of different loci that may be simultaneously analyzed per experiment (Pejic et al 1998). In addition, due to other advantages such as high reproducibility (Jones et al 1997), a high degree of resolution for discriminating closely related germplasms (Gagne et al 2000), no sequence information before the generation of AFLP fingerprints, and high transferability across species, the AFLP marker technique is a very effective, fast, and reliable tool to reveal restriction fragment polymorphisms in contrast to other markers . Many studies have applied this technique not only to DNA fingerprinting and genetic diversity studies (Paul et al 1997;Russell et al 1997;Kremer et al 2005), but also to genome mapping (Becker et al 1995), phylogenetic studies (Aggarwal et al 1999;Zhang et al 2001), and parentage analysis (Lima et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity within and among populations of Shorea leprosula Miq. and Shorea parvifolia Dyer (Dipterocarpaceae) in Indonesia detected by AFLPs

Cao¹,

Finkeldey²,

Siregar³

et al. 2006

Tree Genetics & Genomes

View full text Add to dashboard Cite

The genetic diversity within and among populations of Shorea leprosula and Shorea parvifolia from Indonesia was investigated using amplified fragment length polymorphisms (AFLPs). The results indicated that S. leprosula is genetically more variable than S. parvifolia. At the population level, a higher level of genetic diversity was revealed for S. leprosula with a percentage of polymorphic loci (PPL p ) of 53.32% and an expected heterozygosity (H ep ) of 0.16 in comparison with S. parvifolia showing PPL p of 51.79% and H ep of 0.14. At the species level, S. leprosula showed PPL s of 92.86% and H es of 0.21, while S. parvifolia showed PPL s of 85.71% and H es of 0.21. Genetic differentiation (G st ) indicated that 25 and 31% of total genetic diversity in S. leprosula and S. parvifolia, respectively, were attributed to the differences among populations. An analysis of molecular variance (AMOVA) at two hierarchical levels exhibited that most genetic variation resided within populations with proportion of 70.2% for S. leprosula and 66.2% for S. parvifolia. The AMOVA at three hierarchical levels performed for S. leprosula and S. parvifolia together revealed that the genetic difference between the two species was remarkably higher with a proportion of 44.1% than the differences within and among populations (38.1 and 17.8%, respectively). The genetic differentiation between islands was significant for S. leprosula but not for S. parvifolia. The observed genetic diversity agreed with the life history traits of Shorea species. Highly differentiating individual AFLP markers were found for each species, which will serve as diagnostic markers for the identification of wood of different species, from different islands and regions.

show abstract

“…These results are discussed in relation to the applied uses of RAPD markers in the determination of true O. cumana races instead of populations. Gagne et al (2000) showed that AFLP markers have a higher degree of resolution for discriminating closely related germplasms and appear to be a suitable technique for study of the genetic variability in O. cumana. Gagne et al (2000) showed that AFLP markers have a higher degree of resolution for discriminating closely related germplasms and appear to be a suitable technique for study of the genetic variability in O. cumana.…”

Section: Results and Disscusionmentioning

confidence: 99%

Broomrape in Serbia from its occurrence till today

Maširević

Medić-Pap

2009

Helia

View full text Add to dashboard Cite

Epiphytotic occurrence of broomrape was observed in Serbia for the first time in 1951, then again at the beginning of the 1990s which continues up to date. It is hypothesized that the new race was introduced by confectionery sunflower seed. Sunflower is threatened in the north of the region of Bačka. The main foci of hazard are sunflower fields on the route Subotica -Bačka Topola, with a tendency of spreading toward Senta and Čantavir, while the second direction is toward Čonoplja and Aleksa Šantić, and then to the south. Less intensive attack has also been noticed in Banat around Padej, Itebej and Vršac. It is significant to underline that broomrape has appeared in new localities Svetozar Miletić, Mol, Novi Bečej and Zmajevo in which it had not been registered previously. In 2008, infected areas were found in Bor County (two sunflower plots) near the state border with Romania and Bulgaria.The analysis of the parasitic population showed that broomrape in Serbia belongs to race E of the parasite. Continual monitoring of the broomrape population is important due to changes in race composition and evolution of new, more virulent races. The most reliable method for control of the parasite is cultivation of resistant sunflower hybrids including IMI resistant hybrids.

show abstract

Amplified fragment length polymorphism (AFLP) as suitable markers to study orobanche cumana genetic diversity

Cited by 16 publications

References 12 publications

Genetic diversity of Moroccan populations of Orobanche foetida: evolving from parasitising wild hosts to crop plants

Genetic diversity of Moroccan populations of Orobanche foetida: evolving from parasitising wild hosts to crop plants

Genetic diversity within and among populations of Shorea leprosula Miq. and Shorea parvifolia Dyer (Dipterocarpaceae) in Indonesia detected by AFLPs

Broomrape in Serbia from its occurrence till today

Contact Info

Product

Resources

About