Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles

Chen, Qiang; Tang, Wei; Wang, Dingzhong; Wu, Xing‐De; Li, Na; Liu, Feng

doi:10.1016/j.bios.2010.07.034

Cited by 114 publications

(52 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, Au-nanoparticle labelled aptamers were used in the creation of a highly sensitive quartz crystal microbalance with dissipation monitoring (QCM-D) biosensor for protein detection using a sandwich aptamer/thrombin/Apt-GNPs tertiary configuration, where gold nanoparticles were used for signal amplification. This biosensor resulted improved in sensitivity, selectivity and repeatability [194,195]. Several other studies have also been performed and published on aptamerbased biosensors [196].…”

Section: Aptamers Biosensorsmentioning

confidence: 90%

“…Despite the wide literature on the use of aptamers in biosensors, it seems that most of publications are reviews [197] while the experimental studies are still quite few. These latter studies can be grouped on the basis of aptamers associated to quantum dots (QDs) [142,198], nanoparticles [195,199,200], carbon nanotubes [201], or label-free [202] as compared with antibodiesbased studies [203]. Lee et al (2012) [204] were the only ones, to the best of our knowledge, who described the use of an aptamer-immobilised electrospun polystyrene-poly(styrene-co-maleicanhydride) (PS-PSMA) nanofibre as a new cost-effective aptasensor platform for protein detection (Fig.…”

Section: Aptamers Biosensorsmentioning

confidence: 99%

See 1 more Smart Citation

Electrospinning-Based Nanobiosensors

Cesare

Macagnano

2015

Electrospinning for High Performance Sensors

View full text Add to dashboard Cite

Biological interactions in biosensors occur through different mechanisms, on the basis of the type of biological receptor elements employed therein that confer the biological specificity to the biosensors themselves (biocatalytic, biocomplexing or bioaffinity biosensors). The use of different transduction systems (amperometric, potentiometric, field-effect transistors, piezometric and conductometric) defines the mode of detection. The interest and relevance of nanomaterials in the fabrication of nanobiosensors lie in their extraordinary properties that make them ideally suited and very promising for sensing applications. The resulting nanobiosensors are capable of sensing analytes in traces with fast, precise and accurate biological identification through miniaturised and easy to use systems. These advanced sensors are also characterised by lower detection limits, higher sensitivity values and high stability, and can further offer multi-detection possibilities. These exceptional features make nanobiosensors as the favourite tools in quality control, food safety, and traceability for their capacity of revealing and warning people against the presence of pathogens, toxins and pollutants, as well as bioterrorism agents. Despite the outstanding properties of these nanobiosensors, however, the efficiency of the biorecognition agent and the number of biorecognition sites available for interacting with target analytes can limit the sensitivity of this kind of sensors. The number of available biorecognition sites is directly related to the surface area of the sensor. Electrospinning technology can significantly increase the sensitivity of biosensors by replacing the typical planar interactive surface of conventional biosensors with a mat of electrospun nonwoven nanofibres, thereby taking advantage of the ultrahigh surface area offered by electrospun nanofibres. Moreover, adjusting the electrospinning process to produce

show abstract

Section: Aptamers Biosensorsmentioning

confidence: 90%

Section: Aptamers Biosensorsmentioning

confidence: 99%

Electrospinning-Based Nanobiosensors

Cesare

Macagnano

2015

Electrospinning for High Performance Sensors

View full text Add to dashboard Cite

show abstract

“…In this sense, QCM-D can also contribute to better characterize sensing applications involving DNA which can be understood as yet another type of polymer brush. Layer-by-layer DNA fi lms [11], DNA hybridization [12], Holliday junctions [13], and aptamer-protein interactions [14] have been reported where the dissipation served as a major parameter for this fi lm characterization. Using a small molecule binding aptamer as a model system, Serrano Santos et al not only determined the interaction of adenosine-5´-monophosphate (AMP) with AMP-binding aptamer fi lms using QCM-D, but also succeeded in quantifying in situ and in real-time the structural changes that occur in this particular DNA aptamer when binding to AMP [15].…”

Section: Applications Of Spr and Qcm-dmentioning

confidence: 99%

Sensing Techniques Involving Thin Films for Studying Biomolecular Interactions and Membrane Fouling Phenomena

Diaconu

Schäfer

2014

Smart Membranes and Sensors

View full text Add to dashboard Cite

Interfacial phenomena are ubiquitous in sensing and separation applications involving membranes. In fact, their presence might strongly determine the effi ciency of the overall sensing or separation system, if not even deteriorate its performance with time as is the case in membrane separations (fouling). Characterization of these phenomena at the earliest possible stage is therefore indispensable in order to minimize their detrimental impact. Th is chapter discusses two advanced surface characterization techniques for this purpose, quartz crystal microbalance with dissipation monitoring (QCM-D) and surface plasmon resonance (SPR), and how they can be employed to provide valuable and complementary information on molecular adsorption phenomena occurring at a very early stage on polymer membranes.

show abstract

“…2 Nevertheless, the high nonspecific background encountered in complex media can be efficiently diminished by changing the detection format with double-site interaction, in particular, using sandwich assays. [3][4][5][6][7][8][9][10][11][12][13] Alternatively, the proximity between two aptamers that bind different thrombin epitopes has been exploited to collect a specific signal of the target. [14][15][16][17] Enhanced sensitivity and reduced reaction time have been achieved with bivalent aptamer structures in which distinct aptamers are conjugated with an optimal linker, 18,19 or co-conjugated to the sensor surface with an optimal density.…”

mentioning

confidence: 99%

Thrombin detection in murine plasma using engineered fluorescence resonance energy transfer aptadimers

Trapaidze

Brut

Mazères

et al. 2015

Applied Physics Letters

View full text Add to dashboard Cite

Biodetection strategies, in which two sides of one target protein are targeted simultaneously, have been shown to increase specificity, selectivity, and affinity, and it has been suggested that they constitute excellent candidates for protein sensing in complex media. In this study we propose a method to engineer the sequence of a DNA construct dedicated to reversible thrombin detection. This construct, called Fluorescence Resonance Energy Transfer (FRET) aptadimer, is assembled with two aptamers, which target different epitopes of thrombin, interconnected with a DNA linker that contains a FRET couple and a reversible double helix stem. In the absence of target, the stem is stable maintaining a FRET couple in close proximity, and fluorescence is unquenched upon thrombin addition due to the dehybridization of the stem. We define design rules for the conception of FRET aptadimers, and develop a software to optimize their functionality. One engineered FRET aptadimer sequence is subsequently characterized experimentally by temperature scanning fluorimetry, demonstrating the relevance of our technology for thrombin sensing in bulk and diluted murine plasma.

show abstract

Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles

Cited by 114 publications

References 29 publications

Electrospinning-Based Nanobiosensors

Electrospinning-Based Nanobiosensors

Sensing Techniques Involving Thin Films for Studying Biomolecular Interactions and Membrane Fouling Phenomena

Thrombin detection in murine plasma using engineered fluorescence resonance energy transfer aptadimers

Contact Info

Product

Resources

About