Amplified rDNA Restriction Analysis (ARDRA) for Identification and Phylogenetic Placement of 16S‐rDNA Clones

Sklarz, Menachem Y.; Angel, Roey; Gillor, Osnat; Soares, Ines M.

doi:10.1002/9781118010518.ch7

Cited by 3 publications

(2 citation statements)

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“…PCR products are digested into specific DNA fragments with tetracutter restriction endonucleases. Restriction fragments are then separated on agarose or polyacrylamide gels (Rastogi & Sani, 2011;Sklarz et al, 2011;Cetecioglu et al, 2012). ARDRA is a useful method for rapid monitoring of microbial communities over time, or comparing biodiversity in response to changing environmental conditions.…”

Section: Amplified Ribosomal Rna Restriction Analysismentioning

confidence: 99%

Application of molecular techniques for the assessment of microorganism diversity on cultural heritage objects.

2014

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As a result of their unpredictable ability to adapt to varying environmental conditions, microorganisms inhabit different types of biological niches on Earth. Owing to the key role of microorganisms in many biogeochemical processes, trends in modern microbiology emphasize the need to know and understand the structure and function of complex microbial communities. This is particularly important if the strategy relates to microbial communities that cause biodeterioration of materials that constitute our cultural heritage. Until recently, the detection and identification of microorganisms inhabiting objects of cultural value was based only on cultivation-dependent methods. In spite of many advantages, these methods provide limited information because they identify only viable organisms capable of growth under standard laboratory conditions. However, in order to carry out proper conservation and renovation, it is necessary to know the complete composition of microbial communities and their activity. This paper presents and characterizes modern techniques such as genetic fingerprinting and clone library construction for the assessment of microbial diversity based on molecular biology. Molecular methods represent a favourable alternative to culturedependent methods and make it possible to assess the biodiversity of microorganisms inhabiting technical materials and cultural heritage objects.

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Section: Amplified Ribosomal Rna Restriction Analysismentioning

confidence: 99%

Application of molecular techniques for the assessment of microorganism diversity on cultural heritage objects.

2014

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“…Os perfis resultantes são, então, utilizados para classificar a comunidade em grupos genotípicos específicos ou para a tipagem de linhagens. 29 RISA (ribossomal intergenic space analysis) é um método de análise da comunidade microbiana, que fornece estimativas de diversidade e composição da comunidade. Foi usado originalmente para a comparação da diversidade em solos e mais recentemente para analisar a diversidade microbiana na rizosfera e em ambientes marinhos.…”

Section: Métodos Independentes De Culturaunclassified

Bacterial Biodegradation of Petroleum and Oil Compounds

Tonini¹,

Rezende²,

Grativol³

2011

Revista Virtual De Química

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Petroleum compounds can be degraded by several bacterial species. Thus, these microorganisms are of great interest to the industry and to the recovery of contaminated environments. Methods to isolate, cultivate and identify bacteria able to degrade these pollutants have been developed in the field of chemistry, biochemistry, microbiology and molecular biology, allowing for a better understanding of bacterial communities ecology and dynamics in the environmental context and in the laboratory. Finally, there is a growing need for multidisciplinary research in order to meet the greatest possible number of species, their metabolic properties and their respective roles in the degradation process of oil compounds and, with this information, develop strategies for decontamination of environments polluted with these substances.

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IDENTIFICATION METHODS | DNA Fingerprinting

Säde

Björkroth

2014

Encyclopedia of Food Microbiology

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Amplified rDNA Restriction Analysis (ARDRA) for Identification and Phylogenetic Placement of 16S‐rDNA Clones

Cited by 3 publications

References 32 publications

Application of molecular techniques for the assessment of microorganism diversity on cultural heritage objects.

Application of molecular techniques for the assessment of microorganism diversity on cultural heritage objects.

Bacterial Biodegradation of Petroleum and Oil Compounds

IDENTIFICATION METHODS | DNA Fingerprinting

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