Amplified somatic embryogenesis from male flowers of triploid banana and plantain cultivars (Musa spp.)

Escalant, Jean-Vincent; Teisson, Claude; Côte, François-Xavier

doi:10.1007/bf02823029

Cited by 120 publications

(86 citation statements)

References 9 publications

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“…The choice of auxin is very important in the induction process and can affect the frequency and morphology of somatic embryos (Lazzeri et al 1987, Levi & Sink 1991, Rodriguez & Wetzstein 1994, 1998. Somatic embryogenesis of banana cultivars of different groups has been successfully achieved (Novak et al 1989, Dedh'a et al 1991, Côte et al 1996, Escalant et al 1994, Grapin et al 1996, however, the conversion into plants is frequently low, thus limiting its association with genetic transformation techniques. The characterization of the different stages of the embryogenic process can help to detect possible limiting steps as well as to locate the embryogenic regions in the explant.…”

Section: Introductionmentioning

confidence: 99%

Histological changes in banana explants, cv. Nanicão (Musa spp., Group AAA), submitted to different auxins for induction of somatic embryogenesis

Filippi

Appezzato‐da‐Glória

2001

Rev. bras. Bot.

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-(Histological changes in banana explants, cv. Nanicão (Musa spp. Group AAA), submitted to different auxins for induction of somatic embryogenesis). The present work analyzes the behavior of banana explants, cv. Nanicão (Musa spp. Group AAA) regarding somatic embryogenesis induction treatments with several auxins. Longitudinal segments of shoot meristematic apices of micropropagated banana plantlets cultivated and rooted in vitro were introduced in culture medium containing dicamba, picloram, 2,4-D or NAA in different concentrations. Explant samples were collected at 0, 7 and 10 days and prepared for light microscopy. Histological sections were used for comparison of the histological changes occurring after induction treatment with different auxins. Embryogenic response was observed only in treatments with picloram or dicamba, with distinct embryogenic regions observed at 14 and 21 days in culture, respectively. Histological sections of embryogenic regions of the explant at 26 days in culture revealed the formation of meristematic regions, structures with multiple root meristems, and somatic embryos at early globular stages. Embryo-like structures morphologically similar to Musa balbisiana zygotic embryos were sectioned and showed a lack of apical meristems and absence of procambial differentiation. These results indicate the induction of non-functional somatic embryos and the need for more studies on developmental aspects and maturation treatments for optimization of the process.RESUMO -(Alterações histológicas em explantes de bananeira, cv. Nanicão (Musa spp. Grupo AAA) submetidos a diferentes auxinas para indução de embriogênese somática). O presente trabalho analisa o comportamento de explantes de bananeira 'Nanicão' (Musa spp. Grupo AAA) a tratamentos de indução com diferentes auxinas, visando à obtenção de embriogênese somática. Segmentos longitudinais de ápices meristemáticos caulinares de plântulas de bananeira micropropagadas e enraizadas in vitro foram introduzidos em meio de cultura contendo dicamba, picloram, 2,4-D ou NAA. Amostras dos explantes aos 0, 7 e 10 dias foram coletadas e preparadas para microscopia óptica. Cortes histológicos foram realizados nesses explantes, comparando-se as alterações observadas após os tratamentos com diferentes auxinas. Respostas embriogênicas foram observadas somente nos tratamentos com picloram e dicamba, sendo possível a observação visual de regiões embriogênicas aos 14 e 21 dias de cultivo, respectivamente. Cortes histológicos de regiões embriogênicas dos explantes, aos 26 dias, revelaram a formação de regiões meristemáticas compostas aparentemente por múltiplos meristemas radiculares e embriões nas fases iniciais do estágio globular. Cortes histológicos de estruturas morfologicamente semelhantes a embriões zigóticos de Musa balbisiana revelaram ausência dos meristemas apicais e não diferenciação procambial. Os resultados indicam a indução de embriões somáticos não-funcionais e, consequentemente, a necessidade de maiores estudos visando otimizar o process...

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Section: Introductionmentioning

confidence: 99%

Histological changes in banana explants, cv. Nanicão (Musa spp., Group AAA), submitted to different auxins for induction of somatic embryogenesis

Filippi

Appezzato‐da‐Glória

2001

Rev. bras. Bot.

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“…In Musa, SE has been obtained by different ways. They include cultures of zygotic embryos (Cronauer and Krikorian 1988;Escalant and Teisson 1988), leaf tissues and rhizome (Novak et al 1989a), highly proliferating meristems (Dhed'a et al 1991;Schoofs 1997), male inflorescences (Ma 1991;Escalant et al 1994), and female inflorescences (Grapin et al 1998). The most successful explants were the proliferating meristems and male inflorescences.…”

Section: Somatic Embryogenesis In Musa Sppmentioning

confidence: 99%

“…The immersion frequency was three times daily, during 1 min each according to Escalant et al (1994). For every temporal immersion system, use an inoculant density of 0.5 g fresh weight of somatic embryo (gFW).…”

Section: Germination Of Somatic Embryos In Temporal Immersion Systemmentioning

confidence: 99%

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Mutation Induction Using Gamma Irradiation and Embryogenic Cell Suspensions in Plantain (Musa spp.)

López

Rayas

Santos

et al. 2016

Biotechnologies for Plant Mutation Breeding

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Bananas and plantains (Musa spp.) are among the world's most important crops and are considered as a poor man's crop in tropical and subtropical countries. For this reason, they play a very important role in tropical regions' food security. Several pathogens throughout the tropical areas of the world have become a threat for production, including "black sigatoka" (Mycosphaerella fijiensis Morelet) which is the most damaging disease for Musa plantations in Cuba. It is present in all areas of the country, and it replaced "sigatoka disease" (Mycosphaerella musicola) as the main threat. Chemical control and cultural practices reduce damages, but they are not solving the problem. Genetic variations are the basic tools to develop new cultivars with better traits, such as tolerance against various environmental stresses, resistance against pests and diseases, and improved yield and quality. Tissue culture techniques offer the opportunity for variation induction, handling of large plant populations using established selection methods, and rapidly cloning selected variants. All these strategies can increase the efficiency of mutagenic treatments and subsequent screening of mutant materials. Somatic embryogenesis (SE) is also an excellent system for clonal propagation and mutation induction. The present chapter describes a protocol on how to use embryogenic cell suspensions (ECS) in plantain (Musa spp.) using both in vitro gamma irradiation and plant regeneration in order to achieve genetic improvement. The process involves a series of steps to properly select ECS for irradiation and the posttreatment handling for plant regeneration as well as mutant selection during acclimatization phase and under field conditions.

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“…(Escalant et al, 1994). Sin embargo, la misma contaminación endógena del material no logró controlarse, al mismo tiempo que el proceso de fenolización hizo perder los explantes aun viables.…”

Section: Etapa 1: Establecimiento In Vitrounclassified

Micropropagación de plátano enano (Musa spp) utilizando manos de flores masculinas inmaduras con diferentes concentraciones de desinfectantes y reguladores de crecimiento

Moreno¹

2015

PAyDS

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ResumenEl plátano es un rubro alimenticio de gran importancia económica en el país, considerado como alimento de elevado consumo nacional. Uno de los fines fundamentales del cultivo in vitro -aplicado a la agricultura-es generar aumentos sustanciales en la producción y/o rendimiento de este rubro, a través de nuevos enfoques, permitiendo la expansión de la superficie sembrada. El objetivo principal de la investigación fue establecer un protocolo de micro propagación de plátano enano, usando manos de flores masculinas inmaduras con miras a la reducción de costos de producción in vitro.La investigación inicialmente se llevaría a cabo en dos etapas, pero al final solo se realizó la etapa de establecimiento in vitro (desinfección superficial), debido a que se determinó que de la Musa spp. (Plátano variedad Enano), no es posible obtener plantas a través de inflorescencia masculinas. Esto se debió, en parte, a que los agentes desinfectantes no permitieron obtener explantes libres de contaminantes de patógenos; además de la acción de las polifenoxidasas, las cuales no expresaron la potencialidad de los explantes utilizados para el desarrollo de callos embriogénicos.Palabras Clave: Micropropagación, plátano enano, flores masculinas inmaduras, fenolización, agente desinfectante. AbstractThe plantain is a food industry that has a great economic importance in our country; it is considered as a national high consumed food. One of the fundamental purposes of the in vitro farming applied to agriculture -is to increase the production and / or the performance of this industry through the new approaches, allowing the expansion of the sown field. The main objective of this research was to establish a protocol of micro propagation of the Dwarf Cavendish Banana using handful immature male flowers with the purpose of reducing the in vitro production costs Initially, this research was going to be carried out in two stages, but at the end it was done only the in vitro stage (superficial disinfection) because it was determined from Musa spp (Dwarf Cavendish banana) that it was not possible to get plants through the male inflorescence. This was because, somehow, that the disinfected agents did not allow to get explant free from pollutant pathogens; in addition to the action of the polyphenol oxidases, that did not show the potentiality of the explants used for the development of the embryogenic calli.

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Amplified somatic embryogenesis from male flowers of triploid banana and plantain cultivars (Musa spp.)

Cited by 120 publications

References 9 publications

Histological changes in banana explants, cv. Nanicão (Musa spp., Group AAA), submitted to different auxins for induction of somatic embryogenesis

Histological changes in banana explants, cv. Nanicão (Musa spp., Group AAA), submitted to different auxins for induction of somatic embryogenesis

Mutation Induction Using Gamma Irradiation and Embryogenic Cell Suspensions in Plantain (Musa spp.)

Micropropagación de plátano enano (Musa spp) utilizando manos de flores masculinas inmaduras con diferentes concentraciones de desinfectantes y reguladores de crecimiento

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