AMS function during late pollen meiosis and subsequent pollen wall formation

Ferjentsikova, Ivana; Wilson, Zoe A.

doi:10.1016/j.ydbio.2011.05.257

Cited by 1 publication

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“…Either abnormal development or advanced/delayed PCD of a tapetum leads to impaired pollen wall development and reduced male fertility [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 ]. A genetic transcriptional pathway has been demonstrated to function in regulating the development and PCD of a tapetum, from the upstream to the downstream, including the following transcription factor (TF) genes: dysfunctional tapetum 1 ( DYT1 ), bHLH010/bHLH089/bHLH091 , defective in tapetal development and function 1 ( TDF1 )/ MYB35 , and aborted microspores ( AMS ), MYB103 [ 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ]. Although all of the above TF genes were confirmed to be required for tapetum development, whether they function in pollen wall formation and the possible regulatory pathways remains not very clear.…”

Section: Introductionmentioning

confidence: 99%

bHLH010/089 Transcription Factors Control Pollen Wall Development via Specific Transcriptional and Metabolic Networks in Arabidopsis thaliana

Lai

Wang

Peng

et al. 2022

IJMS

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The pollen wall is a specialized extracellular cell wall that protects male gametophytes from various environmental stresses and facilitates pollination. Here, we reported that bHLH010 and bHLH089 together are required for the development of the pollen wall by regulating their specific downstream transcriptional and metabolic networks. Both the exine and intine structures of bhlh010 bhlh089 pollen grains were severely defective. Further untargeted metabolomic and transcriptomic analyses revealed that the accumulation of pollen wall morphogenesis-related metabolites, including polysaccharides, glyceryl derivatives, and flavonols, were significantly changed, and the expression of such metabolic enzyme-encoding genes and transporter-encoding genes related to pollen wall morphogenesis was downregulated in bhlh010 bhlh089 mutants. Among these downstream target genes, CSLB03 is a novel target with no biological function being reported yet. We found that bHLH010 interacted with the two E-box sequences at the promoter of CSLB03 and directly activated the expression of CSLB03. The cslb03 mutant alleles showed bhlh010 bhlh089–like pollen developmental defects, with most of the pollen grains exhibiting defective pollen wall structures.

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