Amylase inhibitors of pigeonpea (Cajanus cajan) seeds

Giri, Ashok P.; Kachole, Manvendra S.

doi:10.1016/s0031-9422(97)00570-0

Cited by 103 publications

(54 citation statements)

References 27 publications

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“…Insects can digest PIs by altering their genetic makeup by the process of adaptation. They can secrete the proteases that can digest the PI molecules (Michaud 1997;Girard et al 1998;Giri and Kachole 1998;). This situation demands high tolerant PI or the effective approach to compensate the mechanism of acquired resistance .…”

Section: Discussionmentioning

confidence: 99%

Isolation and in vitro identification of proteinase inhibitors from soybean seeds inhibiting helicoverpa gut proteases

Ghodke

Chavan

Sonawane

et al. 2013

Journal of Plant Interactions

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Section: Discussionmentioning

confidence: 99%

Isolation and in vitro identification of proteinase inhibitors from soybean seeds inhibiting helicoverpa gut proteases

Ghodke

Chavan

Sonawane

et al. 2013

Journal of Plant Interactions

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“…Low inhibition potential of HaGPs explained the susceptibility of pigeonpea to H. armigera larvae. Pigeonpea PIs and amylase inhibitors accumulate during seed development, and host defense is inadequate during the early stages of grain development [24,25]. Insects also inactivate host plant defenses by expressing inhibitor resistant or inhibitor degrading proteinases [12,[26][27][28][29][30].…”

Section: Resultsmentioning

confidence: 99%

Protease Inhibitors in Wild Relatives of Pigeonpea against the Cotton Bollworm/Legume Pod Borer, <i>Helicoverpa armigera</i>

Parde¹,

Sharma²,

Kachole³

2012

AJPS

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Cotton bollworm/legume pod borer, Helicoverpa armigera is one of the most damaging pests worldwide. Because of the difficulties associated with chemical control of this pest, emphasis has been placed on developing transgenic plants with resistance to H. armigera. Since toxin genes from the bacterium, Bacillus thuringiensis (Bt) have been deployed on a large scale, there is need to scout for alternate genes which could be deployed alone or in combination with the Bt genes for pest management. Therefore, we evaluated the wild relatives of pigeonpea, which have shown high levels of resistance to this pest, for the protease inhibitors (PIs) under in vivo and in vitro inhibitions. Accessions belonging to Cajanus albicans, C. cajanifolius, C. sericeus, Flemingia bracteata, and Rhynchosia bracteata showed complete inhibition of H. armigera gut proteinases (HaGPs). Some of the C. scarabaeoides accessions (ICPW 116, 152, 278 and 280) exhibited partial inhibition at low concentrations of the PIs. All accessions of wild relatives of pigeonpea showed high to moderate level of inhibition at pH 7.8. Cultivated pigeonpea, ICPL 87 exhibited monomorphism in terms of trypsin inhibitor (TI) and chymotrypsin inhibitor (CTI) isoforms, contrary to the diverse inhibitory profiles of wild pigeonpeas. Cajanus albicans, C. platycarpus, C. scarabaeoides, and R. bracteata showed more number of TI and CTI bands than the cultivated pigeonpea. Protease inhibitor isoforms of wild relatives of pigeonpea showed significant variation in number, band pattern, and protein specificities towards trypsin, chymotrypsin, and H. armigera gut proteinases (HaGPs) as compared to the cultivated pigeonpea. The PIs from the wild relatives of pigeonpea showed considerable potential against the HaGPs, and could be considered as potential candidates for use in genetic transformation of crops for pest management, including H. armigera.

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“…The antinutrient activity of protease inhibitors is associated with growth inhibition and pancreatic hypertrophy. Potential beneficial effects of protease inhibitors remain unclear, although lower incidences of pancreatic cancer have been observed in populations where the intake of soybean and its products is high [33]. While protease inhibitors have been linked with pancreatic cancer in animal studies, they may also act as anticarcinogenic agents.…”

Section: Protease Inhibitorsmentioning

confidence: 99%

Antinutritional Factors in Plant Foods: Potential Health Benefits and Adverse Effects

Gemede¹,

Ratta²

2014

IJNFS

288

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Anti-nutritional factors are compounds which reduce the nutrient utilization and/or food intake of plants or plant products used as human foods and they play a vital role in determining the use of plants for humans. This paper is aimed to review the updated scientific information regarding the potential health benefits and adverse effects associated with major antinutritional factors found in plant foods. Antinutrients in plant foods are responsible for deleterious effects related to the absorption of nutrients and micronutrients. However, some antinutrients may exert beneficial health effects at low concentrations. For example, phytic acid, lectins, tannins, saponins, amylase inhibitors and protease inhibitors have been shown to reduce the availability of nutrients and cause growth inhibition. However, when used at low levels, phytate, lectins, tannins, amylase inhibitors and saponins have also been shown to reduce the blood glucose and insulin responses to starchy foods and/or the plasma cholesterol and triglycerides. In addition, phytates, tannins, saponins, protease inhibitors, goetrogens and oxalates have been related to reduce cancer risks. This implies that anti-nutrients might not always harmful even though lack of nutritive value. Despite of this, the balance between beneficial and hazardous effects of plant bioactives and anti-nutrients rely on their concentration, chemical structure, time of exposure and interaction with other dietary components. Due to this, they can be considered as anti-nutritional factors with negative effects or non-nutritive compounds with positive effects on health.

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Amylase inhibitors of pigeonpea (Cajanus cajan) seeds

Cited by 103 publications

References 27 publications

Isolation and in vitro identification of proteinase inhibitors from soybean seeds inhibiting helicoverpa gut proteases

Isolation and in vitro identification of proteinase inhibitors from soybean seeds inhibiting helicoverpa gut proteases

Protease Inhibitors in Wild Relatives of Pigeonpea against the Cotton Bollworm/Legume Pod Borer, <i>Helicoverpa armigera</i>

Antinutritional Factors in Plant Foods: Potential Health Benefits and Adverse Effects

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Amylase inhibitors of pigeonpea (Cajanus cajan) seeds

Cited by 103 publications

References 27 publications

Isolation and in vitro identification of proteinase inhibitors from soybean seeds inhibiting helicoverpa gut proteases

Isolation and in vitro identification of proteinase inhibitors from soybean seeds inhibiting helicoverpa gut proteases

Protease Inhibitors in Wild Relatives of Pigeonpea against the Cotton Bollworm/Legume Pod Borer, &lt;i&gt;Helicoverpa armigera&lt;/i&gt;

Antinutritional Factors in Plant Foods: Potential Health Benefits and Adverse Effects

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Protease Inhibitors in Wild Relatives of Pigeonpea against the Cotton Bollworm/Legume Pod Borer, <i>Helicoverpa armigera</i>