Amyloid A in Serum and Ascitic Fluid as a Novel Diagnostic Marker of Spontaneous Bacterial Peritonitis

Badawi, Rehab; Asghar, Muhammad Nadeem; Elshweikh, Samah A; Haydara, Tamer; Alnabawy, Sherein M.; Elkadeem, Mahmoud; Elkhalawany, Walaa; Soliman, Samah; Elkhouly, Reham Abdelkader; Soliman, Shimaa E.; Watany, Mona Mohamed; Khalif, Mai; Elfert, Asem

doi:10.2174/1871523018666190401154447

Cited by 14 publications

(10 citation statements)

References 31 publications

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“…When the p value is closer to zero, the enrichment is more remarkable. SAA could be considered a potential marker of Takayasu arteritis, 28 spontaneous bacterial peritonitis, 29 hand, foot, and mouth disease, 30 and early infectious complications after laparoscopic surgery for colorectal cancer. 31 SAA has been reported as a known biomarker of AS for several years, 32,33 but we found that the area under the ROC curve of SAA was 0.604 for active vs stable AS, 0.707 for stable AS vs normal humans, and 0.718 for active AS vs healthy humans (Figure S4A).…”

Section: Discussionmentioning

confidence: 99%

Discovery of Potential Serum Protein Biomarkers in Ankylosing Spondylitis Using Tandem Mass Tag-Based Quantitative Proteomics

Liu

et al. 2020

J. Proteome Res.

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Ankylosing spondylitis (AS) is a systemic, chronic, and inflammatory rheumatic disease that affects 0.2% of the population. Current diagnostic criteria for disease activity rely on subjective Bath Ankylosing Spondylitis Disease Activity Index scores. Here, we aimed to discover a panel of serum protein biomarkers. First, tandem mass tag (TMT)-based quantitative proteomics was applied to identify differential proteins between 15 pooled active AS and 60 pooled healthy subjects. Second, cohort 1 of 328 humans, including 138 active AS and 190 healthy subjects from two independent centers, was used for biomarker discovery and validation. Finally, biomarker panels were applied to differentiate among active AS, stable AS, and healthy subjects from cohort 2, which enrolled 28 patients with stable AS, 26 with active AS, and 28 healthy subjects. From the proteomics study, a total of 762 proteins were identified and 46 proteins were up-regulated and 59 proteins were down-regulated in active AS patients compared to those in healthy persons. Among them, C-reactive protein (CRP), complement factor H-related protein 3 (CFHR3), α-1-acid glycoprotein 2 (ORM2), serum amyloid A1 (SAA1), fibrinogen γ (FG-γ), and fibrinogen β (FG-β) were the most significantly up-regulated inflammation-related proteins and S100A8, fatty acidbinding protein 5 (FABP5), and thrombospondin 1 (THBS1) were the most significantly down-regulated inflammation-related proteins. From the cohort 1 study, the best panel for the diagnosis of active AS vs healthy subjects is the combination of CRP and SAA1. The area under the receiver operating characteristic (ROC) curve was nearly 0.900, the sensitivity was 0.970%, and the specificity was 0.805% at a 95% confidence interval from 0.811 to 0.977. Using 0.387 as the cutoff value, the predictive values reached 92.00% in the internal validation set (62 with active AS vs 114 healthy subjects) and 97.50% in the external validation phase (40 with active AS vs 40 healthy subjects). From the cohort 2 study, a panel of CRP and SAA1 can differentiate well among active AS, stable AS, and healthy subjects. For active AS vs stable AS, the area under the ROC curve was 0.951, the sensitivity was 96.43%, the specificity was 88.46% at a 95% confidence interval from 0.891 to 1, and the coincidence rate was 92.30%. For stable AS vs healthy humans, the area under the ROC curve was 0.908, the sensitivity was 89.29%, the specificity was 78.57% at a 95% confidence interval from 0.836 to 0.980, and the coincidence rate was 83.93%. For active AS vs healthy subjects, the predictive value was 94.44%. The results indicated that the CRP and SAA1 combination can potentially diagnose disease status, especially for active or stable AS, which will be conducive to treatment recommendation for patients with AS.

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Section: Discussionmentioning

confidence: 99%

Discovery of Potential Serum Protein Biomarkers in Ankylosing Spondylitis Using Tandem Mass Tag-Based Quantitative Proteomics

Liu

et al. 2020

J. Proteome Res.

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“…Serum Amyloid-A level has high sensitivity and specificity than ascitic fluid level. 15 A study conducted on patients having culture negative neutrocytic ascites mentioned that it is a variant of SBP and having mortality rate same as SBP. The term culture negative neutrocytic ascites was used in 1984 by Runyon and it is described as increased neutrophil count in ascitic fluid greater than 250 cells/mm 3 , negative ascitic fluid culture and absence of antibiotic therapy in last one month, no intra abdominal source of infection which can be treated surgically or pancreatitis.…”

Section: Resultsmentioning

confidence: 99%

Prevalence of culture negative ascitic fluid infection among patients with chronic liver disease.

Ayub

Hussain

Ahmed

et al. 2020

TPMJ

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Objectives: To determine prevalence of patients with chronic liver disease having culture negative ascitic fluid infection. Study Design: Cross sectional study. Setting: Gastroenterology and medicine ward of DHQ Teaching Hospital Gujranwala. Period: January 2018 August 2018 having total duration of 8 months. Material & Methods: All patients irrespective of age and gender, having ascites due to chronic liver disease and showing signs and symptoms of ascitic fluid infection like fever, abdominal pain and tenderness, admitted in the medical unit of study hospital underwent ascitic tap and fluid was sent for culture examination to the hospital laboratory. Patients with chronic liver disease having no bacterial growth found on culture of ascitic fluid were placed in one group and who had culture positive ascites were placed in separate group. Patients who have taken any antibiotic in last one month or having any intra abdominal source of infection were excluded from the study. All cases in study group were already diagnosed with CLD. Results: There were 160 cases included in this study having chronic liver disease and ascitic fluid infection with 62.5% male and 37.5% female cases. Out of 160 cases, 22.5% were having culture positive ascites while 77.5% cases were having culture negative ascites among them 61% were male and 39% were female in positive group and 62.9% were male and 37.1% were female cases in negative group. Age range of patients was 25-75 years with mean age of 50±25 years. Mean duration of chronic liver disease was 9.5±2.4 months with minimum duration of 6 months and maximum duration of 15 months. There were 72.6% cases with culture negative ascites and 69.4% with positive culture were having age above 45 years. There was majority of male patients (62.9%) having culture negative ascites due to CLD. Conclusion: Ascitic fluid infection among patients with chronic liver disease is usually culture negative. In our study prevalence of culture negative ascitic flud infection was 77.5%, more common among male patients having age above 45 years.

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“…The area under the curve was 0.706 (95% confidence interval: 0.576-0.798). The procalcitonin level was significantly correlated with the (ascitic fluid) white blood cell count (rs=0.404, P<0.01) [20,37]. Cekin et al, reported higher procalcitonin serum levels in patients with positive bacterial culture in ascitic fluid compared to patients without culture positivity [21,38].…”

Section: Discussionmentioning

confidence: 99%

The Role of MicroRNAs (miRNA 155, miRNA-146b) and Procalcitonin as Novel Markers for the Diagnosis of Spontaneous Bacterial Peritonitis

El-Hassib¹,

Abo-Elmatty²,

Mesbah³

et al. 2021

TOBIOMJ

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Background: MircoRNAs are endogenous, small non-coding RNA molecules that have been recognized as important modulators of gene expression. MicroRNA is considered one of the potential biomarkers of infection and inflammation. Our study aims to identify the potential role of miRNA-155, miRNA-146b, and Procalcitonin (PCT) in the early detection of spontaneous bacterial peritonitis in cirrhotic liver patients. miRNA-155 and 146b are molecular biomarkers , while procalcitonin is a serum marker in ascites patients complicated with Spontaneous Bacterial Peritonitis (SBP) . Methods: This study was conducted on 199 patients, 101 of them have ascites complicated with spontaneous bacterial peritonitis, and 98 patients without spontaneous bacterial peritonitis (control group). Ascitic fluid samples were collected from patients with SBP undergoing paracentesis at National Hepatology Institute in Egypt. MicroRNAs were determined in the serum using qPCR (quantitative polymerase chain reaction), while procalcitonin has been assessed in serum samples using ELISA (Enzyme-linked immune assay) technique. Results: Serum levels of miRNA-146b & miRNA-155 were significantly higher (p<0.001) in spontaneous bacterial peritonitis patients (79.2% and 97.0% respectively) than ascites patients (17.3% and 7.1%, respectively). Furthermore, the serum level of procalcitonin was significantly higher (p<0.001) in spontaneous bacterial peritonitis patients than that in ascites patients (68.3% and 27.6%, respectively). Conclusion: miRNA-155, miRNA-146b and procalcitonin can be used as early markers for the detection of SBP in hepatic patients with ascites.

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Amyloid A in Serum and Ascitic Fluid as a Novel Diagnostic Marker of Spontaneous Bacterial Peritonitis

Cited by 14 publications

References 31 publications

Discovery of Potential Serum Protein Biomarkers in Ankylosing Spondylitis Using Tandem Mass Tag-Based Quantitative Proteomics

Discovery of Potential Serum Protein Biomarkers in Ankylosing Spondylitis Using Tandem Mass Tag-Based Quantitative Proteomics

Prevalence of culture negative ascitic fluid infection among patients with chronic liver disease.

The Role of MicroRNAs (miRNA 155, miRNA-146b) and Procalcitonin as Novel Markers for the Diagnosis of Spontaneous Bacterial Peritonitis

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