Amyloid precursor protein interaction network in human testis: sentinel proteins for male reproduction

Yoon, S. Y.; Domingues, Sara C.; Guimarães, S.; Goltsev, A. V.; Silva, Edgar Figueiredo da Cruz e; Mendes, J. F. F.; Silva, Edgar F. da Cruz e; Fardilha, Margarida

doi:10.1186/s12859-014-0432-9

Cited by 37 publications

(22 citation statements)

References 57 publications

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“…Amyloid precursor protein (APP) is known to be ubiquitously expressed in mammalian cells, with a broad tissue distribution and a hallmark of Alzheimer's disease (Silva et al., ). APP protein has been widely studied and reported to be associated with oxidative stress (Muche, Arendt, & Schliebs, ).…”

Section: Discussionmentioning

confidence: 99%

“…APP protein has been widely studied and reported to be associated with oxidative stress (Muche, Arendt, & Schliebs, ). Some studies have shown that amyloid precursor‐like protein 2 (APLP2) is highly expressed in testis and spermatozoa but failed to prove the presence of APP in mammalian spermatozoa (Silva et al., ). APP has been recently demonstrated in rat testis and spermatozoa and localised in the acrosome region and spermatids tail.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic analysis of sperm proteins in infertile men with high levels of reactive oxygen species

et al. 2018

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Oxidative stress is a significant risk factor for male infertility. A pro-oxidant testicular environment may alter the expression profile of functional sperm proteins and result in poor sperm quality. Patients and donors were divided into ROS (-) and ROS (+) groups. Using computational studies, and data mining of available literature on spermatozoa, oxidative stress and proteomics, we identified three core regulatory proteins angiotensin-converting enzyme (ACE), heat-shock protein (Hsp70) family A member 2 (HSPA2) and ribosomal protein subunit 27A (RPS27A) and seven interlink proteins NOS2, SUMO2, UBL4A, FBXO25, MAP3K3, APP and UBC. HSPA2 was validated by Western Blot, while the localisation of ACE, RPS27A, MAP3K3 and APP was identified by immunocytochemistry. The obtained results showed that HSPA2 was 1.2 (ROS+) and 2.1 (ROS-) fold downregulated in spermatozoa from patients with high levels of reactive oxygen species (ROS). ACE and APP were localised in the post-acrosomal region of spermatozoa, whereas RPS27A and MAP3K3 were localised either in the tail or sperm neck area. Our data show that these proteins may play a role in ROS-induced male infertility.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of sperm proteins in infertile men with high levels of reactive oxygen species

et al. 2018

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“…Of the 12 genes that are >1000 fold higher in the male compared to other stages, three are putative metalloendopeptidases. These may be involved in the proteolytic processing of amyloid precursor proteins (APPs), which are integral components of sperm membranes in humans, though their specific roles have not been determined 101 . Also of interest in this small set of highly enriched genes are a few genes commonly, though not exclusively, associated with immunity: a leucine rich immune protein TM, a toll protein, and an apparent homolog of the transcription factor NF-X1 [102][103][104][105] .…”

Section: Belgicamentioning

confidence: 99%

Multi-level analysis of reproduction in the Antarctic midge,Belgica antarctica, identifies female and male accessory gland products that are altered by larval stress and impact progeny viability

Finch

Perrieta

Davis

et al. 2019

Preprint

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Background -The Antarctic midge, Belgica antarctica, is a wingless, non-biting midge endemic to Antarctica. Larval development requires at least two years, but adult life lasts only two weeks. The nonfeeding adults mate in swarms and females die shortly after oviposition. Eggs are suspended in a gel of unknown composition that is expressed from the female accessory gland. This project characterizes molecular mechanisms underlying reproduction in this midge by examining differential gene expression in whole males, females, and larvae, as well as in male and female accessory glands. Functional studies were used to assess the role of the gel encasing the eggs, as well as the impact of stress on reproductive biology.Results -RNA-seq analyses revealed sex-and development-specific gene sets along with those associated with the accessory glands. Proteomic analyses were used to define the composition of the egg-containing gel, which is generated during multiple developmental stages and derived from both the accessory gland and other female organs. Functional studies indicate the gel provides a larval food source and thermal and dehydration buffer, all of which are critical for viability. Larval dehydration stress directly reduces production of storage proteins and key accessory gland components, a feature that impacts adult reproductive success. Modeling reveals that bouts of dehydration may significantly impact population growth. Conclusions -This work lays a foundation for further examination of reproduction in midges andprovides new information related to general reproduction in dipterans. A key aspect is that reproduction and stress dynamics, currently understudied in polar organisms, are likely to prove critical for determining how climate change will alter survivability.

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“…Some proteins in SP have been detected very prone to amyloid formation and amyloid synthesis in the testis and epididymis, and they have been correlated with male infertility [68]. Bovine PDC 109 reported as molecular chaperone, which may stops amyloid formation by binding denatured or unfolded proteins [67,68].…”

Section: Chaperon Like Activitymentioning

confidence: 99%

Gelatin Binding Proteins in Reproductive Physiology

Kumar¹,

Tinson²,

Mulligan³

et al. 2016

Indian J Microbiol

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In order to advance the assisted reproductive technologies used in animals and human beings, it is important to accumulate basic informations about underlying molecular mechanisms that shape the biological processes of reproduction. From within seminal plasma, proteins perform a wide variety of distinct functions that regulate major reproductive events such as fertilization. The ability of such proteins to bind and interact with different antagonistic ions and biomolecules such as polysaccharides, lipids, and other proteins present in the male and female reproductive tract define these capabilities. Over the last two decades, extensive work has been undertaken in an attempt to define the role of seminal plasma proteins, of which, Gelatin binding proteins (GBPs) represent a large family. GBPs comprise of known group of Bovine seminal plasma (BSP) protein family, matrix metallo proteinases (MMP 2 and MMP 9) and fibronectin, which have been widely studied. The presence of a type II repeat is a characteristic feature of GBPs, which is similar in structure to the fibronectin type II domain (fn2), which has ability to bind multiple ligands including gelatin, glycosaminoglycans, choline phospholipids, and lipoproteins. Two fn2 domains are present within the BSP protein family, while, three fn2 domains are found in gelatinases (MMP-2 and MMP9), and ELSPBP1 (Epididymosomes Transfer Epididymal Sperm Binding Protein 1) contains four long fn2 domains. For the most part BSP proteins are exclusively expressed in seminal vesicles although mBSPH1, mBSPH2 and hBSPH1 are all expressed in the epididymis. The expression of gelatinases has been demonstrated in several organs and tissues such as the prostate, testis, epididymis, ovary, human placenta, cervix and endometrial wall. This review intends to bring current updates on the role of GBPs in reproductive physiology to light, which may act as basis for future studies on GBPs.

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Amyloid precursor protein interaction network in human testis: sentinel proteins for male reproduction

Cited by 37 publications

References 57 publications

Proteomic analysis of sperm proteins in infertile men with high levels of reactive oxygen species

Proteomic analysis of sperm proteins in infertile men with high levels of reactive oxygen species

Multi-level analysis of reproduction in the Antarctic midge,Belgica antarctica, identifies female and male accessory gland products that are altered by larval stress and impact progeny viability

Gelatin Binding Proteins in Reproductive Physiology

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