Amyloid-β oligomer detection by ELISA in cerebrospinal fluid and brain tissue

Electrochemical detection of amyloid-β oligomer with the signal amplification of alkaline phosphatase plus electrochemical–chemical–chemical redox cycling

“…The linear regression equation is expressed as Δi pa (μA) = 0.012 + 0.178 [target] (nM) (R 2 = 0.98). The detection limit of the method was estimated to be 3 pM, which is comparable to (or even lower than) that obtained by ELISA (2.2 ng/L) [49], Misfolded Protein Assay (8 ng/L) [10], localized surface plasmon resonance (LSPR) (0.1 pM) [11], SPR (~0.5 μM) [50], square wave voltammetry (48 pM) [20], and electrochemical impedance spectroscopy (100 pM) [22]. The high sensitivity is attributed to the dual amplification of ALP and "outer-sphere to inner-sphere" ECC redox cycling.…”

Prion-Protein-interacting Amyloid-β Oligomers of High Molecular Weight Are Tightly Correlated with Memory Impairment in Multiple Alzheimer Mouse Models

“…The majority of immunoassays currently developed to detect A␤ oligomers rely on pairs of antibodies directed against similar or identical epitopes within the A␤ peptide, thus favoring the detection of multimeric assemblies (15,80,81). Despite low detection limits, these assays cannot effectively distinguish between various forms of A␤o because the selectivity for oligomers is determined simply by number of monomers Ͼ1.…”

A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers

“…6,7 Western blot is a semiquantitative method for special recognition of AβO. 8,9 Examples of quantitative analysis are enzyme-linked immunosorbent assay (ELISA), 10 fluorescent assays, 11,12 surface-enhanced Raman spectroscopy, 13 flow cytometry, 14 surface plasmon resonance nanosensor, 15 and so on. The abovementioned methods usually need expensive monoclonal antibodies, fluorescent dye, or sophisticated instruments.…”