SummaryTo probe the effects of annealing time on the glass transition temperature (T g ) and digestibility of Pueraria lobata (Willd.) Ohwi starch, the starch crystal structure and moisture distribution through the components of P. lobata (Willd.) Ohwi starch were investigated. Annealing times of 0, 1, 3, 6, 12 and 24 h were employed to determine the effect of starch T g using differential scanning calorimetry (DSC) with the support of 1 H low-field NMR, polarised light microscopy and 13 C CP/MAS NMR. The T g values of the starch increased with longer annealing times. The 1 H low-field NMR results showed that the T 2 relaxation time decreased and starch-water interactions increased as the annealing time increased. Compared with native starch, annealed starch had higher contents of slowly digested starch (SDS) and resistant starch (RS). The starch crystal structure was not destroyed after annealing, but the relative crystallinity percentage increased slightly.