Amylolytic activity of IgG and sIgA immunoglobulins from human milk

Savel'ev, Andrew N.; Kanyshkova, Tatyana; Kulminskaya, Anna A.; Buneva, Valentina N.; Eneyskaya, Elena V.; Филатов, М. В.; Nevinsky, Georgy A.; Neustroev, Kirill N.

doi:10.1016/s0009-8981(01)00691-x

Cited by 50 publications

(85 citation statements)

References 39 publications

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“…These Abzs show specific activities significantly higher than Abs found in the sera of AI patients. This is in agreement with the results of [33] that the specific activities of milk amylolytic Abzs were 5-50 times higher than those of Abzs in AI patients.…”

Section: Introductionsupporting

confidence: 93%

“…Chromatography of IgGs under "acid shock" conditions was carried out using FPLC gel-filtration on Superdex 200 HR 10/30 (Pharmacia) using 0.1 M glycine-HCl, pH 2.6, as described previously [26][27][28][29][30][31][32][33]. Fractions collected after the gel-filtration were dialyzed against 20 mM Tris-HCl, pH 8.0, and used in activity assays as described below.…”

Section: Antibody Purificationmentioning

confidence: 99%

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Hydrolysis of myelin basic protein by polyclonal catalytic IgGs from the sera of patients with multiple sclerosis

Polosukhina¹,

Kanyshkova²,

Доронин³

et al. 2004

J Cellular Molecular Medi

117

406

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Catalytically active artificial and natural antibodies (Abs) or abzymes (Abzs) have been studied intensively (see reviews [1][2][3][4][5][6][7]). The first example of a natural Abz was an IgG found in bronchial asthma patients which hydrolyzes intestinal vasoactive peptide (VIP) [8], the second was an IgG with DNase activity in SLE [9], and the third was an IgG with RNase activity in SLE [10].Catalytic IgGs and/or IgMs hydrolyzing RNA and DNA [9][10][11][12][13][14][15][16][17], polysaccharides [18][19] or peptides and proteins [20][21][22][23][24][25] AbstractVarious catalytic antibodies or abzymes have been detected recently in the sera of patients with several autoimmune pathologies, where their presence is most probably associated with autoimmunization. Recently we have shown that DNase, RNase, and polysaccharide-hydrolyzing activities are associated with IgGs from the sera of patients with multiple sclerosis (MS). Here we present evidence demonstrating that highly purified MS IgGs (but not Igs from the sera of healthy individuals) catalyze specifically hydrolysis of human myelin basic protein (hMBP). In contrast to many known proteases, IgGs do not hydrolyze many other different proteins. Specific inhibitors of acidic and thiol proteases have no remarkable effect on proteolytic activity of IgGs. However, specific inhibitor of serine (PMSF, AEBSF, and benzamidin) and metal-dependent (EDTA) proteases significantly inhibit activity of proteolytic abzymes. Interestingly, the ratio of serine-like and metal-dependent activities of MS IgGs varied very much from patient to patient. The findings speak in favor of the generation by the immune systems of individual MS patients of a variety of polyclonal anti-MBP IgGs with different catalytic properties.

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Section: Introductionsupporting

confidence: 93%

Section: Antibody Purificationmentioning

confidence: 99%

Hydrolysis of myelin basic protein by polyclonal catalytic IgGs from the sera of patients with multiple sclerosis

Polosukhina¹,

Kanyshkova²,

Доронин³

et al. 2004

J Cellular Molecular Medi

117

406

View full text Add to dashboard Cite

show abstract

“…7). It is possible that the anti-O6ad Abs may exert bactericidal effects through degradative hydrolysis of LPS, because IgG, IgM, and/or secretory IgA from the milk of healthy women and from the sera of cancer and autoimmune patients as well as pregnant women have been shown to have polysaccharide-hydrolyzing activities (11,(60)(61)(62) or lipase activities. Future work is needed to address whether the anti-O6ad Abs kill target bacteria by enzymatic LPS catalysis.…”

Section: Discussionmentioning

confidence: 99%

Antibody-Dependent Cell-Mediated Cytotoxicity- and Complement-Dependent Cytotoxicity-Independent Bactericidal Activity of an IgG against Pseudomonas aeruginosa O6ad

Xie

McLean

Hall

2010

The Journal of Immunology

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“…Natural Abzs also occur in the milk of healthy human mothers and include sIgA and/or IgG possessing DNase and RNase [25][26][27], amylolitic [28] or ATPase activities [29], and protein kinase [30] and lipid kinase activities [31] which are the first examples of natural Abzs with a synthetic activity. Human IgGs from different AD patients in cleavage of supercoiled (sc) and nicked (n) pBR322 DNA: lanes 1-10 correspond to Abs from the sera of 10 different patients; lane C 1 -DNA incubated alone; lanes C 2 and C 3 -DNA incubated with Ab from the sera of two healthy donors.…”

Section: Natural Catalytic Antibodiesmentioning

confidence: 99%

Catalytic antibodies in healthy humans and patients with autoimmune and viral diseases

Nevinsky

Buneva

2003

J Cellular Molecular Medi

126

404

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Antibodies have been first characterized as proteins produced by the immune system solely for binding other molecules, called antigens, with the goal of eliciting immune response. In this classical conception, antibodies act similarly to enzymes in specific binding to different molecules but cannot catalyze their chemical conversion. However, in 1986 the first monoclonal catalytic antibodies against a chemically stable analog of the transition state of a reaction were obtained and termed abzymes (Abzs). At present, artificial monoclonal Abzs catalyzing more than 100 distinct chemical reactions have been obtained. The discovery of IgG specifically hydrolyzing intestinal vasoactive peptide in the blood serum of asthma patients stimulated studies of natural Abzs. Numerous Abzs discovered afterwards in sera of patients with various autoimmune diseases, viral disorders, or in the milk of healthy mothers, are capable of hydrolyzing proteins, DNA, RNA, polysaccharides, or nucleotides, as well as to phosphorylate proteins and lipids. The phenomenon of catalysis by auto-Abzs is more and more in research focus. In this review we summarize new data on Abzs applications in basic science, medicine and biotechnology.

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Amylolytic activity of IgG and sIgA immunoglobulins from human milk

Cited by 50 publications

References 39 publications

Hydrolysis of myelin basic protein by polyclonal catalytic IgGs from the sera of patients with multiple sclerosis

Hydrolysis of myelin basic protein by polyclonal catalytic IgGs from the sera of patients with multiple sclerosis

Antibody-Dependent Cell-Mediated Cytotoxicity- and Complement-Dependent Cytotoxicity-Independent Bactericidal Activity of an IgG against Pseudomonas aeruginosa O6ad

Catalytic antibodies in healthy humans and patients with autoimmune and viral diseases

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