An ABA-increased interaction of the PYL6 ABA receptor with MYC2 Transcription Factor: A putative link of ABA and JA signaling

Schroeder, Julian; Yazaki, Junshi; Lee, Melissa; Takahashi, Yohei; Kim, Alice Y.; Li, Zixing; Kinoshita, Toshinori; Ecker, Joseph R.; Schroeder, Julian I.

doi:10.1038/srep28941

Cited by 183 publications

(114 citation statements)

References 85 publications

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“…S5B, and Dataset S7). These results point to a possible connection between ABA and JA signaling pathways via an ABA receptor (45). However, the other pairs tested by Y2H assay, such as TGA1-PYL6 and ABI5-PYL6, showed no interaction (SI Appendix, Fig.…”

Section: Halotag-nappa Reveals a Potential Role Of Tfs In Plant Hormonementioning

confidence: 73%

Mapping transcription factor interactome networks using HaloTag protein arrays

Yazaki

Galli

Kim

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Protein microarrays enable investigation of diverse biochemical properties for thousands of proteins in a single experiment, an unparalleled capacity. Using a high-density system called HaloTag nucleic acid programmable protein array (HaloTag-NAPPA), we created high-density protein arrays comprising 12,000 Arabidopsis ORFs. We used these arrays to query protein-protein interactions for a set of 38 transcription factors and transcriptional regulators (TFs) that function in diverse plant hormone regulatory pathways. The resulting transcription factor interactome network, TF-NAPPA, contains thousands of novel interactions. Validation in a benchmarked in vitro pull-down assay revealed that a random subset of TF-NAPPA validated at the same rate of 64% as a positive reference set of literaturecurated interactions. Moreover, using a bimolecular fluorescence complementation (BiFC) assay, we confirmed in planta several interactions of biological interest and determined the interaction localizations for seven pairs. The application of HaloTag-NAPPA technology to plant hormone signaling pathways allowed the identification of many novel transcription factor-protein interactions and led to the development of a proteome-wide plant hormone TF interactome network.protein arrays | interactome | hormone | systems biology | Arabidopsis thalianaA major objective in the postgenomic era is to assign detailed molecular function(s) to the many protein-coding genes that remain uncharacterized even in model organisms such as the reference plant Arabidopsis thaliana (1). It is estimated that Arabidopsis contains more than 2,000 transcription factors and transcriptional regulators (hereafter "TFs"), most of which are uncharacterized (2). TFs function as key players in plant hormone signal transduction pathways and are responsible for directing the widespread changes in gene expression that are essential for the regulation of growth and development in plants (3, 4). The TFs within these transcriptional regulatory networks do not work independently but rather undergo complex interactions with other proteins (2). Understanding how these TFs interact with other proteins will ultimately lead to a greater comprehension of biological systems.For determination of physical protein-protein interactions (PPIs), protein microarrays (5-10) are complementary to other PPI technologies such as the yeast two-hybrid system (Y2H) (11) and protein complex purification coupled with mass spectrometry (AP-MS) (12, 13). With conventional protein microarrays it is necessary to purify thousands of in vivo expressed proteins and to spot these purified proteins on a solid surface (5-8). In contrast, in situ synthesis protein microarray technologies simplify protein microarray fabrication by circumventing the steps of in vivo protein expression and purification (9,10,14,15). This streamlining facilitates an increase in the number of target genes that can be assayed, allowing for thousands of protein-encoding plasmids to be spotted at lower cost and in less time. Such ...

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Section: Halotag-nappa Reveals a Potential Role Of Tfs In Plant Hormonementioning

confidence: 73%

Mapping transcription factor interactome networks using HaloTag protein arrays

Yazaki

Galli

Kim

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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“…The role of PYL8 in regulating lateral root growth is independent of the core ABA signaling pathway, because PYL8 directly interacts with MYB77, and this ABA-induced interaction enhances MYB77-dependent transcription of auxin-responsive genes (Zhao et al, 2014). Similarly, PYL6 interacts with MYC2, a key transcription factor in jasmonate responses, and thus connects ABA and jasmonate pathways (Aleman et al, 2016). Single-gene mutations in most other PYLs do not result in drastic ABA phenotypes.…”

Section: Aba Signalingmentioning

confidence: 99%

Abiotic Stress Signaling and Responses in Plants

Zhu

2016

Cell

3,987

2,749

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Summary As sessile organisms, plants must cope with abiotic stress such as soil salinity, drought, and extreme temperatures. Core stress signaling pathways involve protein kinases related to the yeast SNF1 and mammalian AMPK, suggesting that stress signaling in plants evolved from energy sensing. Stress signaling regulates proteins critical for ion and water transport and for metabolic and gene-expression reprogramming to bring about ionic and water homeostasis and cellular stability under stress conditions. Understanding stress signaling and responses will increase our ability to improve stress resistance in crops to achieve agricultural sustainability and food security for a growing world population.

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“…It was recently shown that the ABA receptor, PYR6, interacts with MYC2. This suggests a close interaction between the ABA and JA pathways (Aleman et al, 2016). ABI5 also seems to participate in the ABA-JA crosstalk.…”

Section: Abi5 As the Integrator Of Aba And Other Phytohormone Signalimentioning

confidence: 99%

The Role and Regulation of ABI5 (ABA-Insensitive 5) in Plant Development, Abiotic Stress Responses and Phytohormone Crosstalk

2016

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ABA Insensitive 5 (ABI5) is a basic leucine zipper transcription factor that plays a key role in the regulation of seed germination and early seedling growth in the presence of ABA and abiotic stresses. ABI5 functions in the core ABA signaling, which is composed of PYR/PYL/RCAR receptors, PP2C phosphatases and SnRK2 kinases, through the regulation of the expression of genes that contain the ABSCISIC ACID RESPONSE ELEMENT (ABRE) motif within their promoter region. The regulated targets include stress adaptation genes, e.g., LEA proteins. However, the expression and activation of ABI5 is not only dependent on the core ABA signaling. Many transcription factors such as ABI3, ABI4, MYB7 and WRKYs play either a positive or a negative role in the regulation of ABI5 expression. Additionally, the stability and activity of ABI5 are also regulated by other proteins through post-translational modifications such as phosphorylation, ubiquitination, sumoylation and S-nitrosylation. Moreover, ABI5 also acts as an ABA and other phytohormone signaling integrator. Components of auxin, cytokinin, gibberellic acid, jasmonate and brassinosteroid signaling and metabolism pathways were shown to take part in ABI5 regulation and/or to be regulated by ABI5. Monocot orthologs of AtABI5 have been identified. Although their roles in the molecular and physiological adaptations during abiotic stress have been elucidated, knowledge about their detailed action still remains elusive. Here, we describe the recent advances in understanding the action of ABI5 in early developmental processes and the adaptation of plants to unfavorable environmental conditions. We also focus on ABI5 relation to other phytohormones in the abiotic stress response of plants.

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An ABA-increased interaction of the PYL6 ABA receptor with MYC2 Transcription Factor: A putative link of ABA and JA signaling

Cited by 183 publications

References 85 publications

Mapping transcription factor interactome networks using HaloTag protein arrays

Mapping transcription factor interactome networks using HaloTag protein arrays

Abiotic Stress Signaling and Responses in Plants

The Role and Regulation of ABI5 (ABA-Insensitive 5) in Plant Development, Abiotic Stress Responses and Phytohormone Crosstalk

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