An abattoir-based study of the prevalence of subclinical Johne's disease in adult cattle in south west England

Abstract

SUMMARYThe prevalance of subclinical Johne's disease was estimated in adult cattle slaughtered at three major abattoirs in south west England. A polymerase chain reaction (PCR) based on IS900 was used to detect Mycobacterium paratuberculosis in intestinal lymph nodes of 1553 cattle. Culture was also carried out on all PCR positive and inconclusive samples. The prevalence of subclinical disease in adult cattle was 3 5 % (95 % confidence intervals (CI) 2 6-47) by PCR and 2 6 % (CI 1-8-3 6) by culture. The proportion of the disease in each month ranged from 1-6 % (CI 02-5 5) in April to 4-6 % (CI 2 8-6 9) in November, but the difference was not significant (P > 005). The proportion of PCR positive lymph nodes in each abattoir ranged from 2-8 % (CI 1 6-4-6) to 4 9 % (CI 2 9-7 6), this difference was not significant either (P > 005). The prevalence in young cattle was 2-0 % (CI 0 6-4A5). The difference between age groups was not statistically significant (P > 005).

Prevalence of Subclinical Paratuberculosis in Dairy Cattle in Uşak Region

“…However, contami-nation of MAP via milk is also very significant and one third of the clinically infected animals are reported to spread MAP via their milks 32 . Relatively lower prevalence of the pTB in milk samples with regard to fecal samples in the present study can be accounted for the fact that the main excretion of MAP is via feces with respect to milk 17 . Since MAP possesses zoonotic potential and also contaminates milk, precise identification of its presence in milk is critical for public health 17 …”

“…These acquired results showed differences according to the prevalence rates obtained using Nested and Outer PCR (Table 1). This result can be explained either with the fact that PCR is more sensitive than culture method 17,28 or with the ability of PCR to be able to replicate residual DNA of dead microorganisms, thereby enabling multiplication of contents of dead microorganisms in our study samples and leading negative growth in cultures 17 . An earlier study indicate that while 50% of PCR positive samples show factor growth in cultures, this percentage decreases to 36% in PCR negative samples 19 .…”

“…Nonetheless, pTB infection generally shows a subclinical course. While the use of complement fixation technique in the determination of the animals showing clinical symptoms is indicated to be more useful 33 , it is reported to be insufficient in the diagnoses of the subclinical pTB cases 17 . Therefore, observation of lower prevalence in other studies with regard to the present study is expected.…”

Seroprevalence of Subclinical Paratuberculosis in Dairy Cattle in Manisa Region of Turkey

“…The presence of paratuberculosis in our country has long been known; yet, the number of scientific research conducted on the prevalence of pTB is quite limited (Fırat 1978, Cetinkaya et al 1999, Civelek et al 2009, Ozturk et al 2010, Yıldırım and Civelek 2013, Makav and Gokce 2013. In the presented study, prevalence of subclinical pTB in Manisa, Turkey where dairy cattle farming is intensive has been investigated serologically using the method ELISA.…”

“…Studies on pTB have been conducted in Turkey and the issue keeps its actuality (Civelek et al 2009, Makav and Gokce 2013, Ozturk et al 2010, Yıldırım and Civelek 2013. Current studies have generally been conducted to demonstrate the regional prevalence of the disease (Cetinkaya et al 1999). However, the nation-wide prevalence of the disease in Turkey could not be fully determined.…”