An able-cryoprotectant and a moderate denaturant: distinctive character of ethylene glycol on protein stability

Naidu, K Tejaswi; Prabhu, N. Prakash

doi:10.1080/07391102.2020.1819422

Journal of Biomolecular Structure and Dynamics

2020

DOI: 10.1080/07391102.2020.1819422

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An able-cryoprotectant and a moderate denaturant: distinctive character of ethylene glycol on protein stability

K Tejaswi Naidu

N. Prakash Prabhu

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Cited by 2 publications

References 91 publications

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An experimental and computational view of the photoionization of diol–water clusters

Wannenmacher,

Lu,

Amarasinghe

et al. 2024

The Journal of Chemical Physics

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In the interstellar medium, diols and other prebiotic molecules adsorb onto icy mantles surrounding dust grains. Water in the ice may affect the reactivity and photoionization of these diols. Ethylene glycol (EG), 1,2-propylene glycol, and 1,3-propylene glycol clusters with water clusters were used as a proxy to study these interactions. The diol–water clusters were generated in a continuous supersonic molecular beam, photoionized by synchrotron-based vacuum ultraviolet light from the Advanced Light Source, and subsequently detected by reflectron time-of-flight mass spectrometry. The appearance energies for the detected clusters were determined from the mass spectra, collected at increasing photon energy. Clusters of both diol fragments and unfragmented diols with water were detected. The lowest energy geometry optimized conformers for the observed EG–water clusters and EG fragment–water clusters have been visualized using density functional theory (DFT), providing insight into hydrogen bonding networks and how these affect fragmentation and appearance energy. As the number of water molecules clustered around EG fragments (m/z 31 and 32) increased, the appearance energy for the cluster decreased, indicating a stabilization by water. This trend was supported by DFT calculations. Fragment clusters from 1,2-propylene glycol exhibited a similar trend, but with a smaller energy decrease, and no trend was observed from 1,3-propylene glycol. We discuss and suggest that the reactivity and photoionization of diols in the presence of water depend on the size of the diol, the location of the hydroxyl group, and the number of waters clustered around the diol.

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An experimental and computational view of the photoionization of diol–water clusters

Wannenmacher,

Lu,

Amarasinghe

et al. 2024

The Journal of Chemical Physics

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Polyols, increasing global stability of cytochrome c, destabilize the thermal unfolding intermediate

Naidu

Prabhu

2021

Journal of Biomolecular Structure and Dynamics

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An able-cryoprotectant and a moderate denaturant: distinctive character of ethylene glycol on protein stability

Cited by 2 publications

References 91 publications

An experimental and computational view of the photoionization of diol–water clusters

An experimental and computational view of the photoionization of diol–water clusters

Polyols, increasing global stability of cytochrome c, destabilize the thermal unfolding intermediate

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