An Accurate Aeration Method for the Determination of Alcohol in Fermentation Mixtures.

Catalytic Vapor-Phase Hydration of Ethylene

“…greatly enhances its activity. (4) Phosphoric acid is an active catalyst but is either volatile or forms volatile compounds at this temperature.…”

“…The problem was to devise some suitable means of purification so that the final liquid to be ana-lyzed was merely a solution of ethanol in water. The final procedure consisted of the following steps: (1) Total liquid product was determined by weighing; (2) acetic acid content was determined by titration of a portion with 0.1 N sodium hydroxide; (3) ethyl acetate was determined by saponification of a portion; (4) the remainder of the product was then distilled several times with ammoniacal silver nitrate containing sodium hydroxide to remove aldehydes and acetic acid and to saponify ethyl acetate; (5) the final distillate was then acidified with sulfuric acid and distilled again; (6) sodium chloride was added to reduce the solubility of ethyl ether, and air was bubbled through the solution held at 31°C. for 30 minutes to volatilize the ether; (7) the solution was distilled to recover ethanol, and the ethanol content of the distillate was determined both by specific gravity and the chromate oxidation method of Dox and Lamb (4), correcting the result for ethanol• resulting from the saponification of ethyl acetate.…”