An acellular aortic matrix of buffalo origin crosslinked with 1‐ethyl‐3‐3‐dimethylaminopropylcarbodiimide hydrochloride for the repair of inguinal hernia in horses

Abstract

An acellular aortic matrix (AAM) crosslinked with 1-ethyl-3-3-dimethylaminopropylcarbodiimide hydrochloride (EDC) was evaluated for the repair of inguinal hernias in 5 horses. The aorta from buffalo was acellularised using 1% sodium dodecyl sulfate (SDS) and 0.25% trypsin. The AAM was crosslinked with 1% EDC. Under anaesthesia, inguinal hernias were repaired with EDC-crosslinked AAM graft using an inlay graft technique. Blood samples collected on Days 0, 15 and 30 post implantation, were used for SDS polyacrylamide gel electrophoresis (PAGE) analysis to assess the animals' serum protein concentration, and gelatin zymography for the identification of matrix metalloproteinases. All animals that underwent hernia repair demonstrated successful healing without clinical signs of wound dehiscence, infection or recurrence during a 6-month follow-up period. SDS-PAGE analysis of serum protein concentrations revealed that, this was increased at Day 15 and had decreased again at Day 30. Gelatin zymography of serum of implanted horses expressed a band of 92 kDa, corresponding to MMP-9 activity. The relative amount of the 92 kDa band was higher at Day 15 as compared to Days 0 and 30. It may be concluded that EDC-crosslinked AAM of buffalo origin can be used safely in horses for the repair of inguinal hernia with adequate strength and minimal foreign body reaction.

Modified acellular porcine corneal matrix in deep lamellar transplantation of rabbit cornea

“…36,37 Crosslinking technology has been reported to preserve microstructure. 19 EDC play an important role in these processes. In this study, we optimized previous preparation protocols for APCM and prepared MAPCM with higher transparency, stability and biocompatibility.…”

“…16,17 Decellularized extracellular matrix are complex meshwork of proteins and glycosaminoglycans (GAGs) contained abundant cross-linkable groups, 18 which crosslinked with EDC was proved to improve its biological properties and post-implantation with minimal foreign body reaction, as EDC crosslinking can resist destruction by cell-removing detergents. 19,20 However, thus far, no research has been reported on the effect of EDC on acellular corneal substitutes.…”

Swim bladder-derived biomaterials: structures, compositions, properties, modifications, and biomedical applications

“…Hence, the direct use of decellularized tissue in xenotransplantation may cause cross-species responses to structural proteins [ 140 ]. Crosslinking can further reduce this response [ 141 , 142 ]. Kumar et al used BDDGE to crosslink the decellularized swim bladder matrix to explore the effect of crosslinking treatment on wound healing [ 115 ].…”