An acetate-independent pathway for isopropanol production via HMG-CoA in Escherichia coli

Potensi Aegiceras corniculatum (L.) Blanco sebagai inhibitor enzim HMGCoA reductase

“…Struktur yang disajikan di sini menggambarkan hal itu statin menghambat HMGR dengan mengikat aktif situs enzim, sehingga mencegah secara sterik substrat dari pengikatan. Ini sangat : first_page -end_page sesuai dengan studi kinetik yang menunjukkan bahwa statin mengandung secara petitif menghambat HMG-CoA tetapi tidak mempengaruhi Pengikatan NADPH [13]. Perbandingan antara terikat substrat dan struktur HMGR yang terikat inhibitor dengan jelas menggambarkan penataan ulang kantong pengikat substrat untuk mengakomodasi molekul statin.…”

Recent Advances on Key Enzymes of Microbial Origin in the Lycopene Biosynthesis Pathway

“…HMGS catalyzes the condensation reaction between acetyl-CoA and acetoacetyl-CoA to produce 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) . The HMGS gene has been identified and successfully cloned from several microorganisms, such as Phaffia rhodozyma, Streptomyces coelicolor, Myxococcus xanthus, Komagataella phaffii, and Enterococcus faecalis, etc. Yang et al constructed recombinant E.…”

“…The characteristics of some key enzymes of lycopene synthesis, such as K m or k cat values were summarized in Table S1, which also indicated that there may be differences in catalytic activity among enzymes from different sources. Moreover, although there is some experimental validation of the properties and catalytic activity of these key enzymes themselves from microorganisms, ,− few relevant studies have been reported, especially ignoring their characteristics, functions, and regulatory roles in multienzyme cascade reactions of the lycopene synthesis pathway, which to some extent indicates that there are some deficiencies in the studies of these key enzymes in the lycopene synthesis pathway that need more attention.…”