An Acid Phosphatase Assay for Quantifying the Growth of Adherent and Nonadherent Cells

Yang, Te-Tuan; Sinai, Parisa; Kain, Steven R.

doi:10.1006/abio.1996.0383

Cited by 145 publications

(122 citation statements)

References 3 publications

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“…Acid phosphate assay was performed 48 hours after the treatment to quantify cell population size as previously described. 12 Moreover, we also quantified cell growth and cell viability by simple cell count using Trypan Blue at 24, 48 and 72 hours after silencing, confirming the acid phosphate assay result.…”

Section: Methodssupporting

confidence: 75%

Identification of thioredoxin reductase 1-regulated genes using small interference RNA and cDNA microarray

Gorreta

Runfola²,

VanMeter³

et al. 2005

Cancer Biology & Therapy

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Thioredoxin reductase 1 (TrxR1) is a cytosolic enzyme that plays a central role in controlling cellular redox homeostasis. TrxR1 can transduce regulatory redox signals through NADPH-dependent reduction of thioredoxin (Trx), which is able to reduce a broad spectrum of target enzymes and regulate the activity of several transcription factors (e.g., p53 and NF-κB). The TrxR1/Trx system is involved in every step of cancer biology, ranging from transformation and progression to invasion, metastasis and resistance to therapy. TrxR1 was also recently identified as one key enzyme involved in cell death induced by interferon-β (IFN-β)/all-trans retinoic acid (ATRA) anti-cancer treatment.Our study employed small interference RNA (siRNA) and microarray techniques to investigate the effect of TrxR1 silencing on gene expression in HepG2 cells. We also investigated TrxR1-mediated cell response to IFN-β/ATRA treatment.We identified TrxR1-dependent genes with functions related to several cellular processes such as apoptosis (SOX4), ubiquitination (Ubiquitin D, F-box protein 25), organization of cytoskeletal/extracellular matrix (Keratin 19, Fibronectin 1) and transport (Cystine/ Glutamate transporter).We also investigated the effect of TrxR1 siRNA on the protein profile using surface enhanced laser desorption ionization time-of-flight (SELDI-TOF) technology. Profiles confirmed significant involvement of TrxR1 in cell response to IFN-β/ATRA.

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Section: Methodssupporting

confidence: 75%

Identification of thioredoxin reductase 1-regulated genes using small interference RNA and cDNA microarray

Gorreta

Runfola²,

VanMeter³

et al. 2005

Cancer Biology & Therapy

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“…Briefly, cells were plated in standard RPMI medium in 96-well plates the day after medium was replaced with media at the indicated pH. Starting from time 0 the plates were collected every 24 h and the acid phosphatase assay was run as previously described (42).…”

Section: Methodsmentioning

confidence: 99%

Autophagy Is a Protective Mechanism for Human Melanoma Cells under Acidic Stress

Marino

Pellegrini

Lernia

et al. 2012

Journal of Biological Chemistry

161

127

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Background: Human melanoma cells are able to sustain low pH conditions characterizing many solid tumors. Results: Acidic stress in melanoma cells induces reduced nutrients uptake, inhibition of mammalian target of rapamycin, and activation of autophagy. Conclusion: Melanoma cells activate autophagy as a protective and adaptive response to acidic stress. Significance: Adaptation to acidosis via autophagy confirms the feasibility of anticancer therapy targeting autophagy.

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“…Acid phosphatase cytotoxicity assays (Yang et al 1996) were used to determine cytotoxicity as per method used by (Stordal et al 2013). Cells were allowed to attach overnight and then received 5-day exposures to drugs.…”

Section: Cytotoxicity Assaysmentioning

confidence: 99%

Carboplatin and taxol resistance develops more rapidly in functional BRCA1 compared to dysfunctional BRCA1 ovarian cancer cells

Busschots

O’Toole

O’Leary

et al. 2015

Experimental Cell Research

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An Acid Phosphatase Assay for Quantifying the Growth of Adherent and Nonadherent Cells

Cited by 145 publications

References 3 publications

Identification of thioredoxin reductase 1-regulated genes using small interference RNA and cDNA microarray

Identification of thioredoxin reductase 1-regulated genes using small interference RNA and cDNA microarray

Autophagy Is a Protective Mechanism for Human Melanoma Cells under Acidic Stress

Carboplatin and taxol resistance develops more rapidly in functional BRCA1 compared to dysfunctional BRCA1 ovarian cancer cells

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