An Acidic Polysaccharide Isolated from the Midrib of Leaves ofNicotiana tabacum

Determination of uronic acids in tobacco by decarboxylation

“…The value for the pectin content of bright lamina estimated by using this uronic acid decarboxylation procedure varied by <9.3% from comparable values obtained by an involved gravimetric procedure and a pectinase-carbazole method. Uronic acid constituents have been determined in suspension-cultured tobacco cells and their extracellular polysaccharides (Kato and Noguchi, 1976; Yamaoka and Sato, 1977; Mori and Kato, 1981; Akiyama and Kato, 1982) and in the laminas and stems (or midribs) of fresh green and flue-cured bright tobaccos (Eda et al, 1977(Eda et al, ,1982Eda and Kato, 1980; Bourne et al, 1967; Bokelman et al, 1983).…”