An Acinetobacter baumannii, Zinc-Regulated Peptidase Maintains Cell Wall Integrity during Immune-Mediated Nutrient Sequestration

Lonergan, Zachery R.; Nairn, Brittany L.; Wang, Jiefei; Hsu, Yen-Pang; Hesse, Laura; Beavers, William N.; Chazin, Walter J.; Trinidad, Jonathan C.; VanNieuwenhze, Michael S.; Giedroc, David P.; Skaar, Eric P.

doi:10.1016/j.celrep.2019.01.089

Cited by 66 publications

(71 citation statements)

References 69 publications

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“…Although the underlying molecular mechanisms remain unclear, media osmolarity affects the growth of cells harboring conditional mutants of division genes, dictates essentiality of FtsEX for division, and modulates cell size in E. coli [44,73,74]. Extracellular metal availability also alters the activity of several nonessential cell wall enzymes [75–77]. Further exploration promises to illuminate additional environmental determinants modulating the activity of not only enzymes involved in bacterial cell wall synthesis, but also other extracytoplasmic processes.…”

Section: Discussionmentioning

confidence: 99%

Environmental pH impacts division assembly and cell size inEscherichia coli

Mueller

Westfall

Levin

2019

Preprint

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Cell size is a complex trait, derived from both genetic and environmental factors. Environmental determinants of bacterial cell size identified to date primarily target assembly of cytosolic components of the cell division machinery. Whether certain environmental cues also impact cell size through changes in the assembly or activity of extracytoplasmic division proteins remains an open question. Here, we identify extracellular pH as a modulator of cell division and a key determinant of cell size across evolutionarily distant bacterial species. In the Gram-negative model organism Escherichia coli, our data indicate environmental pH impacts the length at which cells divide by altering the ability of the terminal cell division protein FtsN to localize to the cytokinetic machinery and activate division. Acidic environments lead to enrichment of FtsN at the septum and activation of division at a reduced cell length, while alkaline pH inhibits FtsN localization and suppress division activation. Altogether, our work reveals a previously unappreciated role for pH in bacterial cell size control.

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Section: Discussionmentioning

confidence: 99%

Environmental pH impacts division assembly and cell size inEscherichia coli

Mueller

Westfall

Levin

2019

Preprint

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“…Although the underlying molecular mechanisms remain unclear, media osmolarity affects the growth of cells harboring conditional mutants of division genes, dictates essentiality of FtsEX for division, and modulates cell size in E. coli [47,87,88]. Extracellular metal availability also alters the activity of several nonessential cell wall enzymes [89][90][91]. Improved understanding of how extracellular processes cope with dynamic environments promises to shed light on how single celled organisms survive-and thrive-across a wide range of ecological niches.…”

Section: The Physical and Chemical Environment As A Mediator Of Extramentioning

confidence: 99%

pH-dependent activation of cytokinesis modulates Escherichia coli cell size

2020

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Cell size is a complex trait, derived from both genetic and environmental factors. Environmental determinants of bacterial cell size identified to date primarily target assembly of cytosolic components of the cell division machinery. Whether certain environmental cues also impact cell size through changes in the assembly or activity of extracytoplasmic division proteins remains an open question. Here, we identify extracellular pH as a modulator of cell division and a significant determinant of cell size across evolutionarily distant bacterial species. In the Gram-negative model organism Escherichia coli, our data indicate environmental pH impacts the length at which cells divide by altering the ability of the terminal cell division protein FtsN to localize to the cytokinetic ring where it activates division. Acidic environments lead to enrichment of FtsN at the septum and activation of division at a reduced cell length. Alkaline pH inhibits FtsN localization and suppresses division activation. Altogether, our work reveals a previously unappreciated role for pH in bacterial cell size control.

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“…Therefore, the ability of these bacteria to compete for metals, both in relation to their host and other microbes, could be general mechanisms that influence host colonization. For instance, iron and zinc homeostasis are essential for colonization of mammalian host tissues by the pathogen Acinetobacter baumannii (Lonergan et al, 2019;Hood et al, 2012). Similarly, root-associated Acinetobacter sp.…”

Section: Discussionmentioning

confidence: 99%

Iron Supplementation Eliminates Antagonistic Interactions Between Root Associated Bacteria

Eng

Herbert

Martinez

et al. 2020

Preprint

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The rhizosphere microbiome (rhizobiome) plays a critical role in plant health and development. However the processes by which the constituent microbes interact to form and maintain a community are not well understood. To investigate these molecular processes, we examined pairwise interactions between 11 different microbial isolates under selected nutrient-rich and nutrient-limited conditions. We observed that when grown with media supplemented with 56 mM glucose, 2 microbial isolates were able to inhibit the growth of 6 out of 11 other microbes tested. The interaction between microbes persisted even after the antagonistic microbe was removed, upon exposure to spent media. To probe the genetic basis for these antagonistic interactions, we used a barcoded transposon library in a proxy bacterium, Pseudomonas putida, to identify genes which showed enhanced sensitivity to the antagonistic factor(s) secreted by Acinetobacter sp. 02. Iron metabolism-related gene clusters in P. putida were implicated by this systems-level analysis. The supplementation of iron prevented the antagonistic interaction in the original microbial pair supporting the hypothesis that iron limitation drives antagonistic microbial interactions between rhizobionts. We conclude that rhizobiome community composition is influenced by competition for limiting nutrients with implications for growth and development of the plant.

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An Acinetobacter baumannii, Zinc-Regulated Peptidase Maintains Cell Wall Integrity during Immune-Mediated Nutrient Sequestration

Abstract: Highlights d During zinc starvation, A. baumannii expresses a putative peptidase named ZrlA d ZrlA is critical for bacterial cell envelope integrity and overcoming Zn limitation d Inactivation of zrlA increases antibiotic efficacy in vitro and during infection

Cited by 66 publications

References 69 publications

Environmental pH impacts division assembly and cell size inEscherichia coli

Environmental pH impacts division assembly and cell size inEscherichia coli

pH-dependent activation of cytokinesis modulates Escherichia coli cell size

Iron Supplementation Eliminates Antagonistic Interactions Between Root Associated Bacteria

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